FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8913476
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8618249.668775683021977No Hit
AAGCTGCCAGTTGAAGAACTGT3874834.347159290045769No Hit
CCCCCCACTGCTAAATTTGACTG3677264.1255061437311324No Hit
TTCAAGTAATCCAGGATAGGCT3550843.983675953129845No Hit
TAGCAGCACGTAAATATTGGCG3188003.5766069264112No Hit
AACATTCAACGCTGTCGGTGAGT2984753.3485814064008252No Hit
TAGCTTATCAGACTGATGTTGAC2400002.6925522658051695No Hit
TAGCTTATCAGACTGATGTTGACT2195952.4636292283728594No Hit
AACATTCAACGCTGTCGGTGAG2044642.2938750269816173No Hit
TGTAAACATCCTTGACTGGAAGCT1421891.5952138088440468No Hit
TATTGCACTTGTCCCGGCCTGTAA1374451.541991025723298No Hit
TAGCTTATCAGACTGATGTTGA1348531.5129114612526022No Hit
TTAATGCTAATCGTGATAGGGGTT1223751.3729211813662818No Hit
TGAGGTAGTAGGTTGTATAGTT1221751.370677387811444No Hit
CCCCCACTGCTAAATTTGACTG1142911.2822270458797442No Hit
TATTGCACTTGTCCCGGCCTGTAT1112181.247751157909664No Hit
CATAAAGTAGAAAGCACTACT984431.1044288445944097No Hit
AGCAGCATTGTACAGGGCTATGA822860.9231639822668508No Hit
TGTAAACATCCCCGACTGGAAGCT694130.778742210109726No Hit
TGAGGTAGTAGATTGTATAGTT673060.7551038450095114No Hit
TGTAAACATCCTACACTCTCAGCT632690.7098128721051137No Hit
TATTGCACTTGTCCCGGCCTGTA585380.6567359355654292No Hit
CAAAGAATTCTCCTTTTGGGCT585310.6566574027910099No Hit
CCCATAAAGTAGAAAGCACTAC582370.6533590262653985No Hit
TATTGCACTTGTCCCGGCCTGT576480.6467510542464018No Hit
CAACGGAATCCCAAAAGCAGCTGT571370.6410181617137916No Hit
TTCAAGTAATTCAGGATAGGTT457050.512762922119272No Hit
ACTGGACTTGGAGTCAGAAGGC452970.5081855832674032No Hit
TAGCTTATCAGACTGATGTTGACA428850.48112543299606125No Hit
CCCATAAAGTAGAAAGCACT397000.4453930206352718No Hit
TGAGAACTGAATTCCATAGGCTGT364580.4090211271113536No Hit
TGAGGTAGTAGTTTGTACAGTT305200.3424028964682241No Hit
CGCCTGTCTGAGCGTCGCT302100.33892501645822576No Hit
AACCGGGCGGAAACACCA293210.3289513541069724No Hit
AACATTCAACGCTGTCGGTGAGTT291860.32743679345745696No Hit
TTAATGCTAATCGTGATAGGGGTTT274970.30848795688685315No Hit
TGTCAGTTTGTCAAATACCCCAA274200.3076240963682407No Hit
CATTGCACTTGTCTCGGTCTGA271720.30484179236024195No Hit
CCCCCACTGCTAAATTTGACTGGCTT268320.30102734331701797No Hit
CAACGGAATCCCAAAAGCAGCT266230.2986825790522126No Hit
GCCTGTCTGAGCGTCGCT263760.29591149401198813No Hit
TTCACAGTGGCTAAGTTCCG250900.2814839014543821No Hit
CAAAGAATTCTCCTTTTGGGCTT239390.2685708695462915No Hit
TGTCAGTTTGTCAAATACCCCA235390.26408328243661616No Hit
ACTGGACTTGGAGTCAGAAGGCA219270.2459983063846248No Hit
CAACACCAGTCGATGGGCTGT210590.23626024235662946No Hit
CACTAGATTGTGAGCTCCTGGAA209200.2347008058360173No Hit
TCGTACGACTCTTAGCGG206980.23221019499014753No Hit
TAGCAGCACGTAAATATTGGC206290.23143608621372852No Hit
AACGGAATCCCAAAAGCAGCTG203090.2278460165259883No Hit
TGTAAACATCCTACACTCAGCT195450.2192747251465085No Hit
TGAGGTAGTAGTTTGTGCTGTT194890.21864646295115397No Hit
CACTAGATTGTGAGCTCCTGGA173840.19503053578648777No Hit
TTCAAGTAATCCAGGATAGGCTT168540.18908448286616802No Hit
ATCACATTGCCAGGGATTTCCA167310.1877045498299429No Hit
TTCTCACTACTGCACTTGACTAG167080.18744651357113656No Hit
TATTGCACTTGTCCCGGCCTGTAG157090.17623876476472253No Hit
TGTAAACATCCCCGACTGGAAGC156370.175430999084981No Hit
TTCAAGTAATCCAGGATAGGC153590.17231212604375667No Hit
CTTCTCACTACTGCACTTGACTAG153190.17186336733278912No Hit
CCCATAAAGTAGAAAGCACTA152100.1706404998454026No Hit
ATCACATTGCCAGGGATTTCC152040.1705731860387575No Hit
AGCAGCATTGTACAGGGCTATGT148490.16659045247892068No Hit
AGCTCGGTCTGAGGCCCCTCAGT146630.16450372447292166No Hit
CACCCGGCTGTGTGCACATGTGC143810.16133997556060062No Hit
ACTGGACTTGGAGTCAGAAGGCT137790.1545861569605393No Hit
CAGTGCAATAGTATTGTCAAAGC137590.15436177760505554No Hit
TTATAAAGCAATGAGACTGATT137160.15387936199076543No Hit
CCCCCCACTGCTAAATTTGACTGGCT136040.15262283760005638No Hit
TATTGCACTTGTCCCGGCCTGTT133430.14969468701099323No Hit
AGCTACATTGTCTGCTGGGTTT133040.1492571472677999No Hit
TATTGCACTTGTCCCGGCCTGTAAT132620.148785950621284No Hit
TGAGGTAGTAGGTTGTGTGGTT131850.14792209010267152No Hit
TACGCCTGTCTGAGCGTCGCT131520.1475518641661233No Hit
TGTCAGTTTGTCAAATACC130530.14644118635647865No Hit
CTACGCCTGTCTGAGCGTCGCT129810.14563342067673712No Hit
TTAATGCTAATCGTGATAGGGGT128650.1443320204149313No Hit
TATTGCACTTGTCCCGGCCTGTAAA125800.14113461459928764No Hit
ATGCACCTGGGCAAGGATTCTGA122640.13758942078264416No Hit
TAGCAGCACGTAAATATTGGCGT119800.1344032339347747No Hit
ACTGGACTTGGAGTCAGAAGG116100.13025221585832508No Hit
TTCACAGTGGCTAAGTTCTG113720.1275821015280683No Hit
AGCTACATCTGGCTACTGGGTCTC112200.12587681842639167No Hit
TCCTGTCTGAGCGTCGCT111170.12472126474565029No Hit
AGCAGCATTGTACAGGGCTATG110560.12403690771142482No Hit
TCGTTTCCCGGCCAATGCACCA110030.12344230241939284No Hit
TGAGGTAGTAGGTTGTATAGT107150.12021123970042664No Hit
TGTAAACATCCTTGACTGGAAGC105410.11825913930771789No Hit
CAAAGTGCTGTTCGTGCAGGTAG102410.11489344897546142No Hit
ACCACAGGGTAGAACCACGGAC102360.11483735413659049No Hit
AACATTCAACGCTGTCGGTGAGA100260.11248137090401095No Hit
TCAGTGCACTACAGAACTTTGT98730.11076486883456016No Hit
AGAGGTAGTAGGTTGCATAGTT97190.10903714779733518No Hit
AGCTACATCTGGCTACTGGGTCT96610.10838644766643227No Hit
TGTGCAAATCCATGCAAAACTG96140.10785915618104541No Hit
AACATTCAACGCTGTCGGTGA95750.10742161643785209No Hit
TCGTTTCCCGGGCGGCGCACCA95530.10717479914681995No Hit
TAGCAGCACATCATGGTTTACA94320.10581730404614316No Hit
CTCCCACTGCTTCACTTGACTAG93910.10535732636740144No Hit
TGTCAGTTTGTCAAATACCCC93830.10526757462520796No Hit
CCCCCCACTGCTAAATTTGACTGG93040.10438127617104709No Hit
TGAGAACTGAATTCCATGGGTT92700.10399983126672468No Hit
CAAAGAATTCTCCTTTTGGGC91620.10278818274711235No Hit
TGAGGTAGTAGGTTGTATAGTTA89970.10093705306437128No Hit
TATTGCACTTGTCCCGGCCTGTTT89230.10010684944908137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)