FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11088466
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG123488111.136626112214259No Hit
CCCCCCACTGCTAAATTTGACTG5454234.918831874490123No Hit
AACATTCAACGCTGTCGGTGAGT4374813.9453699005795753No Hit
TTCAAGTAATCCAGGATAGGCT3939583.552862947859515No Hit
AACATTCAACGCTGTCGGTGAG3018192.7219184330817265No Hit
TAGCAGCACGTAAATATTGGCG2890292.606573352887586No Hit
AAGCTGCCAGTTGAAGAACTGT2493002.2482821338857875No Hit
CCCCCACTGCTAAATTTGACTG1857341.675019790834909No Hit
TATTGCACTTGTCCCGGCCTGTAA1691531.5254860320625052No Hit
TATTGCACTTGTCCCGGCCTGTAT1466001.3220945079328377No Hit
TAGCTTATCAGACTGATGTTGAC1455191.3123456391533328No Hit
TAGCTTATCAGACTGATGTTGA1332781.2019516495789409No Hit
TAGCTTATCAGACTGATGTTGACT1331331.2006439844790073No Hit
TCGTACGACTCTTAGCGG1189471.0727092458055063No Hit
CATAAAGTAGAAAGCACTACT1094160.9867550660298728No Hit
TATTGCACTTGTCCCGGCCTGT1029020.9280093387128572No Hit
TGAGGTAGTAGGTTGTATAGTT869940.7845449496801451No Hit
AGCAGCATTGTACAGGGCTATGA853600.7698089167608937No Hit
TATTGCACTTGTCCCGGCCTGTA758300.6838637553652598No Hit
CCCATAAAGTAGAAAGCACTAC744240.6711839130859039No Hit
ACTGGACTTGGAGTCAGAAGGC642640.5795571722905585No Hit
CCCATAAAGTAGAAAGCACT627070.5655155546312718No Hit
CGCCTGTCTGAGCGTCGCT626570.5650646356312947No Hit
CAACGGAATCCCAAAAGCAGCTGT616880.5563258254117387No Hit
CATTGCACTTGTCTCGGTCTGA586790.5291895199931171No Hit
TTCAAGTAATTCAGGATAGGTT585660.528170443053169No Hit
TGAGGTAGTAGATTGTATAGTT560410.5053990335543258No Hit
TGTAAACATCCTTGACTGGAAGCT519690.4686761901961912No Hit
CAACACCAGTCGATGGGCTGT519450.46845974907620225No Hit
CAACGGAATCCCAAAAGCAGCT502600.45326377877697416No Hit
CACCCGGCTGTGTGCACATGTGC501200.4520012055770383No Hit
ATCCCGGACGAGCCCCCA492840.44446183989742133No Hit
GCCTGTCTGAGCGTCGCT464860.4192284126587032No Hit
TGTAAACATCCCCGACTGGAAGCT444520.400885027739635No Hit
CTCCCACTGCTTCACTTGACTAG404470.36476641584146985No Hit
CACTAGATTGTGAGCTCCTGGA390740.3523841801020989No Hit
CACTAGATTGTGAGCTCCTGGAA373670.33698980544288093No Hit
AACATTCAACGCTGTCGGTGAGTT354220.319449056343772No Hit
CCCCCACTGCTAAATTTGACTGGCTT337200.30409977358455176No Hit
CAAAGAATTCTCCTTTTGGGCT335350.3024313732846365No Hit
TGTAAACATCCTACACTCTCAGCT320650.28917435468530994No Hit
CTACGCCTGTCTGAGCGTCGCT316520.2854497637454992No Hit
TTCACAGTGGCTAAGTTCCG303030.2732839691261172No Hit
TTCTCACTACTGCACTTGACTAG274140.24722986930744073No Hit
CTTCTCACTACTGCACTTGACTAG258960.23353996846813616No Hit
AACGGAATCCCAAAAGCAGCTG251640.2269385143084715No Hit
TCGTTTCCCGGCCAATGCACCA244650.22063466668879173No Hit
TACGCCTGTCTGAGCGTCGCT225850.20368011228965305No Hit
TTCAAGTAATCCAGGATAGGCTT224810.20274220076970073No Hit
CCCATAAAGTAGAAAGCACTA218550.19709669488998746No Hit
CCCCCCACTGCTAAATTTGACTGGCT215620.19445430955012172No Hit
ACTGGACTTGGAGTCAGAAGGCT209490.18892604261040255No Hit
CAAAGAATTCTCCTTTTGGGCTT206880.18657224543052212No Hit
ACTGGACTTGGAGTCAGAAGGCA205480.18530967223058628No Hit
TGTCAGTTTGTCAAATACCCCAA204020.18399298875065315No Hit
AACCGGGCGGAAACACCA197830.17841061153093674No Hit
TATTGCACTTGTCCCGGCCTGTAG195910.1766790825710247No Hit
TTCACAGTGGCTAAGTTCTG194570.1754706196510861No Hit
ACGCCTGTCTGAGCGTCGCT193660.17464994707112777No Hit
ACCACAGGGTAGAACCACGGAC191620.17281019755122123No Hit
TATTGCACTTGTCCCGGCCTGTT190490.171791120611273No Hit
TCCTGTCTGAGCGTCGCT189130.17056462093133531No Hit
AGCTCGGTCTGAGGCCCCTCAGT188410.1699152975713683No Hit
TAGCAGCACGTAAATATTGGC183740.16570371411158225No Hit
TTCAAGTAATCCAGGATAGGC182400.16449525119164365No Hit
TCGTTTCCCGGGCGGCGCACCA180410.16270059357173483No Hit
AGCAGCATTGTACAGGGCTATGT178200.16070753159183607No Hit
TAGCTTATCAGACTGATGTTGACA173840.1567755179120358No Hit
ACTGGACTTGGAGTCAGAAGG169640.15298779831222822No Hit
TGAGGTAGTAGTTTGTACAGTT164020.1479194687524857No Hit
TTCACAGTGGCTAAGTTCCGC156480.14111961023283112No Hit
CAACGGAATCCCAAAAGCAGCTGA152790.1377918280130002No Hit
TGAGGTAGTAGTTTGTGCTGTT146850.1324349102932723No Hit
AGAATTACTCTGAGACCT146170.13182166045330346No Hit
CAAAGTGCTGTTCGTGCAGGT145300.1310370613933433No Hit
TGTAAACATCCCCGACTGGAAGC143500.12941375299342578No Hit
ACCGGGTGCTGTAGGCTTA142500.12851191499347162No Hit
GGAATACCGGGTGCTGTAGGCTTT139180.12551781283362368No Hit
AACATTCAACGCTGTCGGTGA138660.12504885707364752No Hit
AGCAGCATTGTACAGGGCTATG136210.12283935397375977No Hit
TGTCAGTTTGTCAAATACCCCA133690.12056672221387521No Hit
CAAAGTGCTGTTCGTGCAGGTAG133000.11994445399390682No Hit
TGTAAACATCCTACACTCAGCT131830.11888930353396042No Hit
TATTGCACTTGTCCCGGCCTGTAAT129090.11641826741408597No Hit
TTCACAGTGGCTAAGTTCTGC128860.1162108446740965No Hit
TGATGTAGTAGGTTGTATAGTT128350.11575090729411985No Hit
CTCCCACTGCTTCACTTGACTAGCCT127300.11480397739416796No Hit
CCCCCCACTGCTAAATTTGACTGG127280.11478594063416889No Hit
ATCCCACCGCTGCCACCA125680.11334299983424218No Hit
TATTGCACTTGTCCCGGCCTGA123790.11163852601432876No Hit
TATTGCACTTGTCCCGGCCTGTTT122920.11085392695436862No Hit
TTATAAAGCAATGAGACTGATT120090.10830172541449827No Hit
TCAGTGCACTACAGAACTTTGT117770.10620946125460455No Hit
ACTTGACTTGGAGTCAGAAGGC116070.10467633665468244No Hit
AACATTCAACGCTGTCGGTGAGA113250.10213315349481163No Hit
ATCACATTGCCAGGGATTTCC112400.10136659119485059No Hit
TAGCAGCACATCATGGTTTACA112240.1012222971148579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)