FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7661019
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG86141711.244156945701349No Hit
CAACGGAATCCCAAAAGCAGCTG6053897.902199433260772No Hit
AACATTCAACGCTGTCGGTGAGT3162004.127388275632785No Hit
CCCCCACTGCTAAATTTGACTG2619273.4189577130666295No Hit
AAGCTGCCAGTTGAAGAACTGT2559343.340730521618599No Hit
TTCAAGTAATCCAGGATAGGCT2406933.1417883182380826No Hit
TAGCAGCACGTAAATATTGGCG2122882.7710151874052262No Hit
AACATTCAACGCTGTCGGTGAG2039932.6627397739125827No Hit
TAGCTTATCAGACTGATGTTGACT1902602.4834816360591194No Hit
TATTGCACTTGTCCCGGCCTGTAA1633172.131792128436178No Hit
TATTGCACTTGTCCCGGCCTGTAT1588122.072987940638184No Hit
TAGCTTATCAGACTGATGTTGAC1528631.9953350853195901No Hit
TAGCTTATCAGACTGATGTTGA1036381.3527965405124305No Hit
TGTAAACATCCTTGACTGGAAGCT701540.9157267460112031No Hit
TATTGCACTTGTCCCGGCCTGT663770.866425210536614No Hit
TATTGCACTTGTCCCGGCCTGTA661420.8633577334816687No Hit
CATAAAGTAGAAAGCACTACT617100.8055064215347854No Hit
TGAGGTAGTAGGTTGTATAGTT545240.7117068891227133No Hit
CCCATAAAGTAGAAAGCACTAC466400.6087962972027612No Hit
AGCAGCATTGTACAGGGCTATGA442440.5775210843361699No Hit
CAACGGAATCCCAAAAGCAGCTGT407720.5322007424860844No Hit
TGTAAACATCCCCGACTGGAAGCT402570.5254783991529064No Hit
TTAATGCTAATCGTGATAGGGGTT383910.5011213260272557No Hit
TGAGGTAGTAGATTGTATAGTT371890.4854315072185567No Hit
TTCAAGTAATTCAGGATAGGTT344240.4493397027210088No Hit
TTCTCACTACTGCACTTGACTAG317320.4142007740745715No Hit
ACTGGACTTGGAGTCAGAAGGC297740.3886428163146443No Hit
CCCCCACTGCTAAATTTGACTGGCTT288640.37676450091038804No Hit
CAACGGAATCCCAAAAGCAGCT288160.37613795240554815No Hit
CCCATAAAGTAGAAAGCACT285020.37203928093638716No Hit
TCGTACGACTCTTAGCGG253440.33081761055546266No Hit
TGTAAACATCCTACACTCTCAGCT249400.3255441606397269No Hit
TAGCTTATCAGACTGATGTTGACA246460.32170655104758256No Hit
CTCCCACTGCTTCACTTGACTAG240080.31337867717075235No Hit
CTTCTCACTACTGCACTTGACTAG235970.30801385559806077No Hit
CAAAGAATTCTCCTTTTGGGCT231570.3022704943036951No Hit
AACATTCAACGCTGTCGGTGAGTT223820.292154346569301No Hit
CATTGCACTTGTCTCGGTCTGA222750.2907576655272621No Hit
GGAATACCGGGTGCTGTAGGCTTT208670.27237890938529197No Hit
TATTGCACTTGTCCCGGCCTGTAG197520.2578247097416153No Hit
CAACACCAGTCGATGGGCTGT184930.24139086458341902No Hit
CCCCCCACTGCTAAATTTGACTGGCT177230.2313399823182791No Hit
CACCCGGCTGTGTGCACATGTGC175070.2285205140464996No Hit
CCCCCCACTGCTAAATTTGACTGG170900.223077373910703No Hit
CACTAGATTGTGAGCTCCTGGAA169480.22122383458388498No Hit
CACTAGATTGTGAGCTCCTGGA160190.2090975103964629No Hit
CCCCCCACTGCTAAATTTGACTGTA159990.20883644851944627No Hit
CCCCCCACTGCTAAATTTGACTGT150940.19702339858444418No Hit
ACCCCCCACTGCTAAATTTGACTG150660.19665791195662094No Hit
TATTGCACTTGTCCCGGCCTGTT150610.19659264648736677No Hit
TGAGGTAGTAGTTTGTACAGTT148270.19353822252627229No Hit
AACGGAATCCCAAAAGCAGCTG148240.1934990632447198No Hit
TTCAAGTAATCCAGGATAGGCTT145440.1898441969664871No Hit
TATTGCACTTGTCCCGGCCTGTAAT142650.18620238378210524No Hit
TTCACAGTGGCTAAGTTCCG141850.18515813627403874No Hit
TCGTTTCCCGGCCAATGCACCA137290.17920592547805977No Hit
TAGCAGCACGTAAATATTGGC134990.17620371389236864No Hit
GGAATACCGGGTGCTGTAGGCTT134890.17607318295386032No Hit
CAAAGAATTCTCCTTTTGGGCTT129650.16923336177602485No Hit
TATTGCACTTGTCCCGGCCTGTTT127500.16642694659809615No Hit
CCCATAAAGTAGAAAGCACTA125090.16328115098004586No Hit
CGCCTGTCTGAGCGTCGCT116120.1515725257958504No Hit
ACTGGACTTGGAGTCAGAAGGCA115240.15042385353697726No Hit
CTCCCACTGCTTCACTTGACTAGCCT114130.148974960119535No Hit
TATTGCACTTGTCCCGGCCTGTAAA111750.1458683237830372No Hit
AGCTCGGTCTGAGGCCCCTCAGT111720.14582916450148473No Hit
TGTAAACATCCCCGACTGGAAGC106410.1388979716666934No Hit
TTCAAGTAATCCAGGATAGGC106110.13850637885116848No Hit
TGAGGTAGTAGTTTGTGCTGTT103920.1356477512978365No Hit
ACTGGACTTGGAGTCAGAAGGCT102350.13359841556325602No Hit
ACCGGGTGCTGTAGGCTTA102020.1331676634661786No Hit
AGCAGCATTGTACAGGGCTATGT99360.12969554050185753No Hit
AACATTCAACGCTGTCGGTGA98830.12900372652776346No Hit
TCAGTGCACTACAGAACTTTGT97680.1275026207349179No Hit
ATGCACCTGGGCAAGGATTCTGA96610.12610593969287898No Hit
AACATTCAACGCTGTCGGTGAGA90720.11841766741473947No Hit
ACCGGGTGCTGTAGGCTT89680.11706014565425304No Hit
ATCACATTGCCAGGGATTTCC86850.11336612009446784No Hit
TGTCAGTTTGTCAAATACCCCA86690.11315727059285456No Hit
CAACGGAATCCCAAAAGCAGCTGA83220.10862784702661617No Hit
ACCGGGTGCTGTAGGCTTT82700.10794908614637296No Hit
CCCCCACTGCTAAATTTGACTGGCTA81940.10695705101370978No Hit
ATCCCGGACGAGCCCCCA79190.10336745020473125No Hit
TGAGAACTGAATTCCATGGGTT79080.10322386617237211No Hit
CCCCCCACTGCTAAATTTGACTGA78390.10232320269666477No Hit
ACCACAGGGTAGAACCACGGAC78240.1021274062889023No Hit
TTATAAAGCAATGAGACTGATT77400.10103094640543249No Hit
TCGTTTCCCGGGCGGCGCACCA77150.10070461905916171No Hit
TTCACAGTGGCTAAGTTCTG76910.10039134480674178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)