FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7222395
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6531469.04334365539409No Hit
CAACGGAATCCCAAAAGCAGCTG6187278.566784286929751No Hit
AACATTCAACGCTGTCGGTGAGT4112835.694551461115046No Hit
TTCAAGTAATCCAGGATAGGCT2601373.601810756681129No Hit
AACATTCAACGCTGTCGGTGAG2531253.5047238485294696No Hit
AAGCTGCCAGTTGAAGAACTGT2518953.487693486717356No Hit
CCCCCACTGCTAAATTTGACTG2380223.2956103896283713No Hit
TAGCAGCACGTAAATATTGGCG2073062.870322102294322No Hit
TAGCTTATCAGACTGATGTTGACT1324781.834266887922912No Hit
TATTGCACTTGTCCCGGCCTGTAA1135581.5723039241138155No Hit
TAGCTTATCAGACTGATGTTGAC1102991.5271803882230202No Hit
TATTGCACTTGTCCCGGCCTGTAT1062581.471229419050052No Hit
TAGCTTATCAGACTGATGTTGA1017391.408660146668799No Hit
CATAAAGTAGAAAGCACTACT852611.180508681676923No Hit
TATTGCACTTGTCCCGGCCTGT757991.0494995081271516No Hit
TATTGCACTTGTCCCGGCCTGTA537070.7436175949944582No Hit
TTAATGCTAATCGTGATAGGGGTT534490.7400453727606978No Hit
TGTAAACATCCTTGACTGGAAGCT465360.644329201047575No Hit
TGAGGTAGTAGGTTGTATAGTT461700.6392616299717753No Hit
TTCAAGTAATTCAGGATAGGTT402540.5573497434022925No Hit
CCCATAAAGTAGAAAGCACTAC389810.5397240112178855No Hit
CAACGGAATCCCAAAAGCAGCTGT374050.5179029947822018No Hit
AGCAGCATTGTACAGGGCTATGA337650.46750420047643476No Hit
CCCATAAAGTAGAAAGCACT336930.4665073012484086No Hit
CGCCTGTCTGAGCGTCGCT310500.42991279208628164No Hit
CAACACCAGTCGATGGGCTGT297370.4117332269974157No Hit
TGTAAACATCCCCGACTGGAAGCT292480.4049626197404047No Hit
TTCTCACTACTGCACTTGACTAG287860.39856584969390346No Hit
AACATTCAACGCTGTCGGTGAGTT261970.3627190149527961No Hit
TGAGGTAGTAGATTGTATAGTT259160.3588283387989718No Hit
CATTGCACTTGTCTCGGTCTGA240570.3330889545642408No Hit
TCGTACGACTCTTAGCGG226490.3135940363272848No Hit
ACTGGACTTGGAGTCAGAAGGC216840.3002328175072119No Hit
CCCCCACTGCTAAATTTGACTGGCTT179870.2490448113125909No Hit
ATCCCGGACGAGCCCCCA165570.22924528497818242No Hit
CTCCCACTGCTTCACTTGACTAG163400.22624074147149248No Hit
CTTCTCACTACTGCACTTGACTAG155860.21580099122244073No Hit
TTCACAGTGGCTAAGTTCCG154570.21401488010556058No Hit
TGTCAGTTTGTCAAATACCCCAA140330.19429842870682093No Hit
TAGCTTATCAGACTGATGTTGACA140080.1939522831415341No Hit
TTCAAGTAATCCAGGATAGGCTT138870.19227693860554568No Hit
TATTGCACTTGTCCCGGCCTGTT138540.191820026459367No Hit
CAAAGAATTCTCCTTTTGGGCT137460.19032467761732777No Hit
CACCCGGCTGTGTGCACATGTGC136670.1892308576310213No Hit
TGTAAACATCCTACACTCTCAGCT135400.18747243815936404No Hit
TAGCAGCACGTAAATATTGGC133360.18464789034662324No Hit
AACATTCAACGCTGTCGGTGA133360.18464789034662324No Hit
TGATGTAGTAGGTTGTATAGTT128890.17845880763929417No Hit
TATTGCACTTGTCCCGGCCTGTAG124180.1719374251892897No Hit
TTCAAGTAATCCAGGATAGGC122670.16984670597495707No Hit
ACCGGGTGCTGTAGGCTT122320.16936210218355546No Hit
TGAGAACTGAATTCCATGGGTT114260.15820236915870706No Hit
AACGGAATCCCAAAAGCAGCTG113990.15782853194819724No Hit
TGAGGTAGTAGTTTGTACAGTT113760.15751007802813333No Hit
CTACGCCTGTCTGAGCGTCGCT112290.15547474210424658No Hit
CACTAGATTGTGAGCTCCTGGA111770.1547547593284499No Hit
CCCCCCACTGCTAAATTTGACTGG109800.15202713227398945No Hit
TTCACAGTGGCTAAGTTCTG107080.14826106852366838No Hit
AACATTCAACGCTGTCGGTGAGA106490.1474441649895914No Hit
TTATAAAGCAATGAGACTGATT103130.14279196859213597No Hit
ACCGGGTGCTGTAGGCTTA102910.14248736049468355No Hit
CCCCCCACTGCTAAATTTGACTGGCT101900.14108893241092463No Hit
ACCGGGTGCTGTAGGCTTT101410.1404104871029624No Hit
CCCCCCACTGCTAAATTTGACTGT99250.13741978941888391No Hit
CCCATAAAGTAGAAAGCACTA97280.13469216236442344No Hit
ACCCCCCACTGCTAAATTTGACTG96630.1337921838946776No Hit
CACTAGATTGTGAGCTCCTGGAA95290.13193684366474No Hit
CAAAGAATTCTCCTTTTGGGCTT92480.12804616751091571No Hit
CCCCCCACTGCTAAATTTGACTGTA92140.12757540954212557No Hit
CAAAGTGCTGTTCGTGCAGGT91840.12716003486378133No Hit
TGAGGTAGTAGTTTGTGCTGTT91200.12627390221664697No Hit
ACCACAGGGTAGAACCACGGAC87460.12109556455995553No Hit
ACGCCTGTCTGAGCGTCGCT87400.12101248962428668No Hit
TTCACAGTGGCTAAGTTCCGC85480.11835409168288358No Hit
TATTGCACTTGTCCCGGCCTGTAAT83210.11521108995007888No Hit
TGTAAACATCCCCGACTGGAAGC82580.114338803125556No Hit
CAACGGAATCCCAAAAGCAGCT81480.11281576263829379No Hit
TACGCCTGTCTGAGCGTCGCT80790.11186040087810206No Hit
CTCCCACTGCTTCACTTGACTAGCCT80680.11170809682937584No Hit
TGTCAGTTTGTCAAATACCCCA79710.11036505203606284No Hit
ACTGGACTTGGAGTCAGAAGGCT78450.10862047838701705No Hit
ACTTGACTTGGAGTCAGAAGGC77570.1074020459972073No Hit
AGCAGCATTGTACAGGGCTATGT75910.10510363944370253No Hit
TTAATGCTAATCGTGATAGGGGTTT74630.1033313741494338No Hit
ATCCCACCGCTGCCACCA74510.10316522427809613No Hit
AGCTCGGTCTGAGGCCCCTCAGT73940.10237601238924207No Hit
TTCACAGTGGCTAAGTTCTGC72650.10058990127236186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)