FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8528702
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6863608.047648985742496No Hit
CCCCCCACTGCTAAATTTGACTG6705887.862720493692945No Hit
AACATTCAACGCTGTCGGTGAGT4032344.727964466339661No Hit
TTCAAGTAATCCAGGATAGGCT3110743.64737799491646No Hit
AAGCTGCCAGTTGAAGAACTGT2682983.1458245346126525No Hit
AACATTCAACGCTGTCGGTGAG2594953.0426083593963065No Hit
CCCCCACTGCTAAATTTGACTG2517572.9518794301876183No Hit
TAGCAGCACGTAAATATTGGCG1961212.299541008702145No Hit
TATTGCACTTGTCCCGGCCTGTAA1290601.5132431640828816No Hit
TAGCTTATCAGACTGATGTTGAC1288171.5103939614726836No Hit
TAGCTTATCAGACTGATGTTGACT1244811.4595538688067657No Hit
TATTGCACTTGTCCCGGCCTGTAT1213581.4229363389645926No Hit
TATTGCACTTGTCCCGGCCTGT943791.1066044985508932No Hit
TAGCTTATCAGACTGATGTTGA890951.0446489981711167No Hit
CATAAAGTAGAAAGCACTACT865861.015230688092983No Hit
TATTGCACTTGTCCCGGCCTGTA773070.9064333587924633No Hit
TTAATGCTAATCGTGATAGGGGTT729610.8554760149903232No Hit
TGAGGTAGTAGGTTGTATAGTT648120.759928064082905No Hit
CCCATAAAGTAGAAAGCACTAC556770.6528191511439841No Hit
TGTAAACATCCTTGACTGGAAGCT468130.5488877439966832No Hit
CAACGGAATCCCAAAAGCAGCTGT458460.5375495591240027No Hit
TGAGGTAGTAGATTGTATAGTT406870.477059697947003No Hit
CCCATAAAGTAGAAAGCACT373130.43749916458565447No Hit
AGCAGCATTGTACAGGGCTATGA360570.4227724218761542No Hit
CGCCTGTCTGAGCGTCGCT360370.42253791960370995No Hit
TTCAAGTAATTCAGGATAGGTT321240.3766575499999883No Hit
CAACGGAATCCCAAAAGCAGCT317770.37258893557308015No Hit
TGTAAACATCCCCGACTGGAAGCT301280.35325422321005No Hit
GCCTGTCTGAGCGTCGCT300540.35238656480200625No Hit
ACTGGACTTGGAGTCAGAAGGC280120.3284438827854461No Hit
CAACACCAGTCGATGGGCTGT278000.32595815869753686No Hit
CATTGCACTTGTCTCGGTCTGA272370.3193569197282306No Hit
AACATTCAACGCTGTCGGTGAGTT271380.3181961334796315No Hit
TTCTCACTACTGCACTTGACTAG268460.31477240030194514No Hit
CTCCCACTGCTTCACTTGACTAG226560.26564417422487036No Hit
TCGTACGACTCTTAGCGG225230.26408473411311595No Hit
TCGTTTCCCGGCCAATGCACCA222780.2612120812756736No Hit
CCCCCACTGCTAAATTTGACTGGCTT219730.25763592162089843No Hit
TGTAAACATCCTACACTCTCAGCT193850.22729132756660977No Hit
CACCCGGCTGTGTGCACATGTGC192220.22538013404618898No Hit
ACCGGGTGCTGTAGGCTT184890.21678562576110647No Hit
CTACGCCTGTCTGAGCGTCGCT182480.21395987337815298No Hit
TTCACAGTGGCTAAGTTCCG182290.2137370962193309No Hit
ATCCCGGACGAGCCCCCA178480.20926982792926754No Hit
TAGCTTATCAGACTGATGTTGACA175140.20535363997944822No Hit
AACGGAATCCCAAAAGCAGCTG168610.19769714078414277No Hit
TATTGCACTTGTCCCGGCCTGTT165300.1938161281751901No Hit
TATTGCACTTGTCCCGGCCTGTAG163510.19171733283681386No Hit
TGAGGTAGTAGTTTGTACAGTT158420.18574925000310716No Hit
TTCAAGTAATCCAGGATAGGC158230.18552647284428508No Hit
CTTCTCACTACTGCACTTGACTAG154670.18135233239477708No Hit
ACCGGGTGCTGTAGGCTTT153560.18005084478271136No Hit
CAAAGAATTCTCCTTTTGGGCT150200.1761112066056476No Hit
CCCCCCACTGCTAAATTTGACTGG148600.17423518842609345No Hit
TGTCAGTTTGTCAAATACCCCAA147520.17296887615489437No Hit
ACCGGGTGCTGTAGGCTTA140380.1645971450286339No Hit
TTCAAGTAATCCAGGATAGGCTT137760.16152516525961394No Hit
TAGCAGCACATCATGGTTTACA137510.16123203741905862No Hit
GGAATACCGGGTGCTGTAGGCTTT134760.1580076311729499No Hit
TAGCAGCACGTAAATATTGGC133870.15696409606057288No Hit
ACCCCCCACTGCTAAATTTGACTG133750.1568233946971063No Hit
AATGCACCCGGGCAAGGATTCT132120.1549122011766855No Hit
TACGCCTGTCTGAGCGTCGCT129260.15155881868073243No Hit
TTCACAGTGGCTAAGTTCCGC128700.15090221231788847No Hit
TTCACAGTGGCTAAGTTCTGC128010.15009317947795572No Hit
CACCCGTAGAACCGACCTTGCG125460.14710327550429128No Hit
AACATTCAACGCTGTCGGTGA124540.14602456505104763No Hit
TGAGGTAGTAGTTTGTGCTGTT123010.14423062266684894No Hit
CCCATAAAGTAGAAAGCACTA122190.14326916334982745No Hit
CCCCCCACTGCTAAATTTGACTGGCT121930.14296431039564989No Hit
AACATTCAACGCTGTCGGTGAGA116690.13682035085760996No Hit
ACGCCTGTCTGAGCGTCGCT114380.13411184961087866No Hit
CGCGACCTCAGATCAGAC113970.1336311199523679No Hit
CCCCCCACTGCTAAATTTGACTGTA112940.1324234332492799No Hit
CACTAGATTGTGAGCTCCTGGA111670.13093434381925878No Hit
ATCACATTGCCAGGGATTTCC107250.12575184359824038No Hit
TTAATGCTAATCGTGATAGGGGTTT107230.12572839337099598No Hit
TTCACAGTGGCTAAGTTCTG104380.1223867359886651No Hit
CCCCCCACTGCTAAATTTGACTGT103530.12139010133077695No Hit
AGCTCGGTCTGAGGCCCCTCAGT102730.12045209224099986No Hit
ATCCCACCGCTGCCACCA101900.11947890781035612No Hit
GGAATACCGGGTGCTGTAGGCTT98740.11577377190573665No Hit
CACTAGATTGTGAGCTCCTGGAA98320.11528131713360369No Hit
ACCACAGGGTAGAACCACGGAC98290.11524614179273705No Hit
TATTGCACTTGTCCCGGCCTGA98280.11523441667911483No Hit
ACTGGACTTGGAGTCAGAAGGCT95420.11188103418316175No Hit
TGAGGTAGTAGGTTGTGTGGTT95070.11147065520638429No Hit
TCCTGTCTGAGCGTCGCT91010.10671025907576558No Hit
CAAAGTGCTGTTCGTGCAGGT90620.10625297964449924No Hit
CAAAGAATTCTCCTTTTGGGCTT88290.10352102817052349No Hit
ACTGGACTTGGAGTCAGAAGGCA88020.10320445010272372No Hit
TATTGCACTTGTCCCGGCCTGTTT87960.10313409942099044No Hit
TGTAAACATCCTACACTCAGCT86980.10198503828601352No Hit
TGTCAGTTTGTCAAATACCCCA86830.1018091615816803No Hit
TATTGCACTTGTCCCGGCCTGTAAT86620.10156293419561382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)