FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7824289
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7284109.309599888245437No Hit
CAACGGAATCCCAAAAGCAGCTG5523507.059427380558157No Hit
AACATTCAACGCTGTCGGTGAGT3166444.046936405339833No Hit
CCCCCACTGCTAAATTTGACTG2420283.0932906491567476No Hit
AAGCTGCCAGTTGAAGAACTGT2317542.9619815934713043No Hit
TTCAAGTAATCCAGGATAGGCT2283022.917862568726692No Hit
AACATTCAACGCTGTCGGTGAG2127832.7195186680860077No Hit
TAGCAGCACGTAAATATTGGCG1942472.482615353292804No Hit
TAGCTTATCAGACTGATGTTGACT1372811.7545491992946578No Hit
TAGCTTATCAGACTGATGTTGAC1279441.6352156726317242No Hit
TATTGCACTTGTCCCGGCCTGTAT1270461.6237385914554026No Hit
TATTGCACTTGTCCCGGCCTGTAA1257841.6076093303813295No Hit
TAGCTTATCAGACTGATGTTGA968601.237939958506134No Hit
TATTGCACTTGTCCCGGCCTGT778860.9954386909788224No Hit
TATTGCACTTGTCCCGGCCTGTA717790.917386870551433No Hit
TTAATGCTAATCGTGATAGGGGTT691840.8842209177089446No Hit
GCCTGTCTGAGCGTCGCT655820.8381847858636101No Hit
CATAAAGTAGAAAGCACTACT653470.8351813180724792No Hit
TGTAAACATCCTTGACTGGAAGCT576370.7366420130953751No Hit
TGAGGTAGTAGGTTGTATAGTT541270.6917817069384835No Hit
TCGTACGACTCTTAGCGG427010.5457492687194965No Hit
AGCAGCATTGTACAGGGCTATGA422700.5402407809834223No Hit
CCCATAAAGTAGAAAGCACTAC415790.5314093076060968No Hit
TTCAAGTAATTCAGGATAGGTT365100.4666238683157025No Hit
TGTAAACATCCCCGACTGGAAGCT347980.44474328593946366No Hit
TGAGGTAGTAGATTGTATAGTT345990.44219992385250595No Hit
CAACGGAATCCCAAAAGCAGCTGT345310.4413308353001787No Hit
TTCTCACTACTGCACTTGACTAG329520.42115008788657987No Hit
CAACGGAATCCCAAAAGCAGCT306410.39161385782145824No Hit
ACTGGACTTGGAGTCAGAAGGC287570.3675349926363916No Hit
CCCATAAAGTAGAAAGCACT262500.3354937426263268No Hit
CATTGCACTTGTCTCGGTCTGA246390.31490401236457394No Hit
CTCCCACTGCTTCACTTGACTAG240670.30759344395382127No Hit
AACATTCAACGCTGTCGGTGAGTT235390.30084522695928023No Hit
CTTCTCACTACTGCACTTGACTAG214250.27382679755310674No Hit
TAGCTTATCAGACTGATGTTGACA201020.25691791292473987No Hit
CAACACCAGTCGATGGGCTGT200390.25611272794243667No Hit
CCCCCACTGCTAAATTTGACTGGCTT199530.2550135865380228No Hit
CAAAGAATTCTCCTTTTGGGCT193340.2471023245690439No Hit
CGCCTGTCTGAGCGTCGCT192960.24661665743686106No Hit
TTCACAGTGGCTAAGTTCCG176790.22595024289107932No Hit
TGTAAACATCCTACACTCTCAGCT175070.22375196008225157No Hit
TCCTGTCTGAGCGTCGCT169170.2162113388194122No Hit
TATTGCACTTGTCCCGGCCTGTT166200.2124154667599829No Hit
TATTGCACTTGTCCCGGCCTGTAG161730.20670248759983176No Hit
CCCCCCACTGCTAAATTTGACTGG157990.20192250056203187No Hit
TCGTTTCCCGGCCAATGCACCA156230.1996730948971849No Hit
CACCCGGCTGTGTGCACATGTGC151880.19411348430509148No Hit
ACCGGGTGCTGTAGGCTT150400.19222193863237927No Hit
ATCCCGGACGAGCCCCCA148360.18961467297539752No Hit
TGAGGTAGTAGTTTGTACAGTT146260.1869307230343869No Hit
GGAATACCGGGTGCTGTAGGCTTT144580.1847835630815784No Hit
ACCGGGTGCTGTAGGCTTA143580.18350549168109714No Hit
CCCCCCACTGCTAAATTTGACTGTA142030.1815244810103512No Hit
TAGCAGCACGTAAATATTGGC139340.17808646894305669No Hit
ACCCCCCACTGCTAAATTTGACTG136870.174929632583868No Hit
AACGGAATCCCAAAAGCAGCTG136080.17391995617748782No Hit
CACTAGATTGTGAGCTCCTGGA134020.17128712909249647No Hit
ACCGGGTGCTGTAGGCTTT132360.1691655305676976No Hit
TGTCAGTTTGTCAAATACCCCAA131080.16752959917508162No Hit
TTAATGCTAATCGTGATAGGGGTTT130210.1664176770566629No Hit
CCCCCCACTGCTAAATTTGACTGT129470.16547190422030678No Hit
CCCCCCACTGCTAAATTTGACTGGCT128530.16427051710385443No Hit
CACTAGATTGTGAGCTCCTGGAA126080.16113924217267536No Hit
TTCAAGTAATCCAGGATAGGCTT123500.15784181795943375No Hit
CCCATAAAGTAGAAAGCACTA117090.14964938028234898No Hit
CTCCCACTGCTTCACTTGACTAGCCT112930.144332603256347No Hit
TGAGAACTGAATTCCATGGGTT110890.14172533759936526No Hit
TGAGGTAGTAGTTTGTGCTGTT110680.1414569426052642No Hit
ACTGGACTTGGAGTCAGAAGGCA109960.1405367311969177No Hit
CCCACCACTGCTAAATTTGACTG108720.13895192266032097No Hit
GGAATACCGGGTGCTGTAGGCTT107060.1368303241355221No Hit
TTCAAGTAATCCAGGATAGGC105370.13467038346870877No Hit
CAAAGAATTCTCCTTTTGGGCTT102700.13125793282942388No Hit
CCACCCACTGCTAAATTTGACTG102450.13093841497930353No Hit
TCGTTTCCCGGGCGGCGCACCA100720.128727351456471No Hit
TATTGCACTTGTCCCGGCCTGTTT99860.12762821005205713No Hit
AGCTCGGTCTGAGGCCCCTCAGT98240.1255577343832775No Hit
CTACGCCTGTCTGAGCGTCGCT97490.12459918083291656No Hit
TGTAAACATCCCCGACTGGAAGC97170.12419019798476257No Hit
AGCAGCATTGTACAGGGCTATGT97040.12402404870270002No Hit
TGTCAGTTTGTCAAATACCCCA96820.12374287299459415No Hit
ACTGGACTTGGAGTCAGAAGGCT94240.12044544878135252No Hit
AACCGGGCGGAAACACCA93000.11886064024475579No Hit
TATTGCACTTGTCCCGGCCTGTAAT92540.11827272740053442No Hit
TTCACAGTGGCTAAGTTCTG92460.11817048168849592No Hit
AACATTCAACGCTGTCGGTGAGA91320.1167134802919473No Hit
CACCCCACTGCTAAATTTGACTG91280.11666235743592805No Hit
TTAATGCTAATCGTGATAGGGGT91250.1166240152939136No Hit
AACATTCAACGCTGTCGGTGA90370.1154993124614901No Hit
TATTGCACTTGTCCCGGCCTGA85940.1098374561573582No Hit
ACCACAGGGTAGAACCACGGAC85650.10946681545121863No Hit
ACTGGACTTGGAGTCAGAAGG83000.10607992623994335No Hit
TTATAAAGCAATGAGACTGATT82780.10579875053183746No Hit
CAAAGTGCTGTTCGTGCAGGT79250.10128715848813866No Hit
TGAGGTAGTAGGTTGTGTGGTT79120.1011210092060761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)