FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5894917
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG59544910.101058250692928No Hit
CAACGGAATCCCAAAAGCAGCTG4230707.176861014328106No Hit
TTCAAGTAATCCAGGATAGGCT1939963.2909029932058416No Hit
AACATTCAACGCTGTCGGTGAGT1774823.0107633406882575No Hit
CCCCCACTGCTAAATTTGACTG1706962.8956472160676734No Hit
AAGCTGCCAGTTGAAGAACTGT1679882.8497093343298983No Hit
TAGCTTATCAGACTGATGTTGACT1477292.506040373426801No Hit
TATTGCACTTGTCCCGGCCTGTAA1356732.3015251953505027No Hit
TAGCAGCACGTAAATATTGGCG1293162.1936865268840937No Hit
AACATTCAACGCTGTCGGTGAG1245232.1123791904109934No Hit
TATTGCACTTGTCCCGGCCTGTAT1234212.0936851188914107No Hit
TAGCTTATCAGACTGATGTTGAC1230362.0871540684966385No Hit
TAGCTTATCAGACTGATGTTGA726391.232231089937314No Hit
TGTAAACATCCTTGACTGGAAGCT610391.035451389731187No Hit
TATTGCACTTGTCCCGGCCTGTA519120.8806230859569355No Hit
TATTGCACTTGTCCCGGCCTGT458130.7771610694433866No Hit
CCCCCACTGCTAAATTTGACTGGCTT422210.7162272174485239No Hit
TGTAAACATCCCCGACTGGAAGCT400330.6791104946855061No Hit
CATAAAGTAGAAAGCACTACT355080.6023494478378576No Hit
CCCATAAAGTAGAAAGCACTAC354220.6008905638535708No Hit
TGAGGTAGTAGGTTGTATAGTT342340.580737608349702No Hit
TTAATGCTAATCGTGATAGGGGTT337700.5728664203414569No Hit
AGCAGCATTGTACAGGGCTATGA325010.551339399689597No Hit
CAAAGAATTCTCCTTTTGGGCT320970.5444860377169009No Hit
CAACGGAATCCCAAAAGCAGCTGT294960.5003632790758547No Hit
TAGCTTATCAGACTGATGTTGACA245670.41674887025550994No Hit
TTCTCACTACTGCACTTGACTAG237280.40251626952508407No Hit
TTCAAGTAATTCAGGATAGGTT235620.3997002841600653No Hit
ACTGGACTTGGAGTCAGAAGGC234380.3975967770199309No Hit
TGTAAACATCCTACACTCTCAGCT203200.34470375070590475No Hit
TGAGGTAGTAGATTGTATAGTT201970.3426172073330294No Hit
CTTCTCACTACTGCACTTGACTAG185090.3139823681995862No Hit
CCCCCCACTGCTAAATTTGACTGGCT180370.3059754700532679No Hit
CTCCCACTGCTTCACTTGACTAG178780.30327823105906326No Hit
TATTGCACTTGTCCCGGCCTGTAG167260.28373597117652377No Hit
CCCCCCACTGCTAAATTTGACTGG160840.27284523259615023No Hit
ACCCCCCACTGCTAAATTTGACTG158950.2696390805841711No Hit
CACTAGATTGTGAGCTCCTGGAA154190.26156432736881624No Hit
GTCAGGATGGCCGAGCGGTCTAAGG146540.24858704541556734No Hit
CCCATAAAGTAGAAAGCACT143170.2428702558492342No Hit
AACATTCAACGCTGTCGGTGAGTT143040.24264972687486525No Hit
CCCCCCACTGCTAAATTTGACTGTA137530.23330269111507423No Hit
TATTGCACTTGTCCCGGCCTGTAAA133690.22678860448756108No Hit
CATTGCACTTGTCTCGGTCTGA130170.22081735841234068No Hit
TAGCAGCACATCATGGTTTACA128830.2185442135996147No Hit
CAAAGAATTCTCCTTTTGGGCTT127050.2155246630274862No Hit
TATTGCACTTGTCCCGGCCTGTAAT124210.21070695312588794No Hit
TTCACAGTGGCTAAGTTCCG122800.20831506194234797No Hit
CACTAGATTGTGAGCTCCTGGA120110.2037518085496369No Hit
TATTGCACTTGTCCCGGCCTGTT115500.1959315118431693No Hit
GGAATACCGGGTGCTGTAGGCTTT113740.19294588880555907No Hit
CACCCGGCTGTGTGCACATGTGC113240.19209770044260166No Hit
CTCCCACTGCTTCACTTGACTAGCCT108010.18322565016606682No Hit
TAGCAGCACGTAAATATTGGC105040.1781874112900996No Hit
TATTGCACTTGTCCCGGCCTGTTT102910.1745741288639009No Hit
TGTAAACATCCCCGACTGGAAGC102810.1744044911913094No Hit
TTCAAGTAATCCAGGATAGGCTT99090.16809396977090602No Hit
CCCCCACTGCTAAATTTGACTGGCTA94210.15981565134844136No Hit
CAACACCAGTCGATGGGCTGT92400.15674520947453544No Hit
TCGTACGACTCTTAGCGG90700.15386136904048014No Hit
CCCCCCACTGCTAAATTTGACTGT89920.15253819519426653No Hit
ATCACATTGCCAGGGATTTCC87290.14807672440511038No Hit
AGCTCGGTCTGAGGCCCCTCAGT86800.14724549980941207No Hit
ACTGGACTTGGAGTCAGAAGGCA86500.14673658679163762No Hit
ACTGGACTTGGAGTCAGAAGGCT86460.146668731722601No Hit
AACGGAATCCCAAAAGCAGCTG85590.14519288397105506No Hit
ATGCACCTGGGCAAGGATTCTGA83580.14178316675196614No Hit
TGAGGTAGTAGTTTGTACAGTT82220.1394760944047219No Hit
CGGGCCGCCGGTGAAATACCACTACT80600.1367279641087398No Hit
TTCAAGTAATCCAGGATAGGC79500.1348619497102334No Hit
TGAGGTAGTAGTTTGTGCTGTT78410.1330128990789862No Hit
CTACGCCTGTCTGAGCGTCGCT77930.13219863825054703No Hit
ATCACATTGCCAGGGATTTCCA77270.13107902961144322No Hit
GGAATACCGGGTGCTGTAGGCTT77200.13096028324062917No Hit
CTACGGGGATGATTTTACGAACTGAA75230.12761842109057683No Hit
CCCATAAAGTAGAAAGCACTA72140.12237661700749985No Hit
CAACGGAATCCCAAAAGCAGCT70780.1200695446602556No Hit
TGTAAACATCCTACACTCAGCT70750.12001865335847817No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC69320.11759283464041986No Hit
TTCACAGTGGCTAAGTTCCGC69050.11713481292442286No Hit
TCGTTTCCCGGCCAATGCACCA66820.11335189282563267No Hit
CCCCCACTGCTAAATTTGACTGGCT66050.11204568274667819No Hit
TTAATGCTAATCGTGATAGGGGTTT63840.1082966901824063No Hit
TGTCAGTTTGTCAAATACCCCA63750.10814401627707396No Hit
AGCAGCATTGTACAGGGCTATGT62960.1068038786636012No Hit
AACATTCAACGCTGTCGGTGAGA62600.10619318304227184No Hit
TGTCAGTTTGTCAAATACCCCAA61860.10493786426509483No Hit
CGCCTGTCTGAGCGTCGCT61160.1037504005569544No Hit
TCAGGCTCAGTCCCCTCCCGAT60800.10313970493562505No Hit
CAAAGAATTCTCCTTTTGGGC60520.10266471945236887No Hit
CCCCCCACTGCTAAATTTGACTGA60460.10256293684881398No Hit
CGCGACCTCAGATCAGACGTGGCG60410.10247811801251823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)