FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053959
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG102009216.84999848859234No Hit
CAACGGAATCCCAAAAGCAGCTG4849328.01016326671522No Hit
AACATTCAACGCTGTCGGTGAGT2534594.186665287954543No Hit
CCCCCACTGCTAAATTTGACTG2302103.802635597631236No Hit
TTCAAGTAATCCAGGATAGGCT1890843.12331153877983No Hit
TAGCAGCACGTAAATATTGGCG1667882.755023613473431No Hit
AAGCTGCCAGTTGAAGAACTGT1640982.7105898801098585No Hit
AACATTCAACGCTGTCGGTGAG1481942.4478857554205438No Hit
TATTGCACTTGTCCCGGCCTGTAA1411562.331631251549606No Hit
TATTGCACTTGTCCCGGCCTGTAT1273372.103367399746183No Hit
TAGCTTATCAGACTGATGTTGACT1239992.0482299268957718No Hit
TAGCTTATCAGACTGATGTTGAC1044291.7249703871466588No Hit
TAGCTTATCAGACTGATGTTGA726281.1996777645834735No Hit
TGTAAACATCCTTGACTGGAAGCT506820.8371711800492867No Hit
TATTGCACTTGTCCCGGCCTGT454390.7505666952815505No Hit
TATTGCACTTGTCCCGGCCTGTA449130.7418781660067404No Hit
TGAGGTAGTAGGTTGTATAGTT442320.730629328675665No Hit
TGTAAACATCCCCGACTGGAAGCT344290.5687022327042519No Hit
CATAAAGTAGAAAGCACTACT336070.5551243409477996No Hit
AGCAGCATTGTACAGGGCTATGA324950.5367561954086574No Hit
CCCATAAAGTAGAAAGCACTAC324830.536557978010753No Hit
CTTCTCACTACTGCACTTGACTAG311420.5144071837949349No Hit
CAACGGAATCCCAAAAGCAGCTGT304670.5032574551628116No Hit
TGAGGTAGTAGATTGTATAGTT303110.5006806289900543No Hit
TTCAAGTAATTCAGGATAGGTT273840.45233210201786966No Hit
TTCTCACTACTGCACTTGACTAG253250.41832130016077085No Hit
ACTGGACTTGGAGTCAGAAGGC248790.4109542202053235No Hit
CTCCCACTGCTTCACTTGACTAG235480.3889686071544257No Hit
CACCCGGCTGTGTGCACATGTGC234690.387663675951555No Hit
CAAAGAATTCTCCTTTTGGGCT231350.38214662504321556No Hit
CAACGGAATCCCAAAAGCAGCT228420.37730681691104945No Hit
CCCCCACTGCTAAATTTGACTGGCTT226830.374680436388816No Hit
CCCCCCACTGCTAAATTTGACTGGCT215120.3553377219766437No Hit
ACCCCCCACTGCTAAATTTGACTG210990.34851573986543355No Hit
TGTAAACATCCTACACTCTCAGCT182360.30122437234873906No Hit
CCCCCCACTGCTAAATTTGACTGG176470.2914952017349308No Hit
TAGCTTATCAGACTGATGTTGACA174940.28896792991164955No Hit
TATTGCACTTGTCCCGGCCTGTAG166160.2744650236316433No Hit
AACATTCAACGCTGTCGGTGAGTT164630.2719377518083621No Hit
CCCATAAAGTAGAAAGCACT162850.26899752707277996No Hit
CAAAGAATTCTCCTTTTGGGCTT148500.24529402990671065No Hit
CATTGCACTTGTCTCGGTCTGA147860.24423687045122044No Hit
CTCCCACTGCTTCACTTGACTAGCCT147490.24362570014101517No Hit
CACTAGATTGTGAGCTCCTGGAA135380.22362226106916153No Hit
TCGTACGACTCTTAGCGG132390.21868334423804323No Hit
TATTGCACTTGTCCCGGCCTGTAAT130350.2153136484736682No Hit
CACTAGATTGTGAGCTCCTGGA125530.20735191632450767No Hit
TGAGGTAGTAGTTTGTACAGTT124050.20490723508368655No Hit
TTCAAGTAATCCAGGATAGGCTT108640.17945281756946158No Hit
TATTGCACTTGTCCCGGCCTGTAAA107420.17743760735743339No Hit
TCGTTTCCCGGCCAATGCACCA106890.17656214718335556No Hit
TATTGCACTTGTCCCGGCCTGTTT99850.16493339317296335No Hit
TAGCAGCACGTAAATATTGGC97120.16042394737063795No Hit
TGTAAACATCCCCGACTGGAAGC96640.1596310777790203No Hit
TATTGCACTTGTCCCGGCCTGTT96630.15961455966252827No Hit
CAACACCAGTCGATGGGCTGT95710.1580948929452611No Hit
CCCCCCACTGCTAAATTTGACTGT90780.14995146151468816No Hit
AACGGAATCCCAAAAGCAGCTG89190.1473250809924547No Hit
AACATTCAACGCTGTCGGTGA88150.14560719687728313No Hit
CCCCCCACTGCTAAATTTGACTGTA87810.14504558091655395No Hit
AGCTCGGTCTGAGGCCCCTCAGT85420.14109775107495773No Hit
AACATTCAACGCTGTCGGTGAGA82590.13642312410771198No Hit
ATGCACCTGGGCAAGGATTCTGA82110.13563025451609434No Hit
ACTGGACTTGGAGTCAGAAGGCA80140.13237618556716357No Hit
TGTCAGTTTGTCAAATACCCCAA77490.12799888469677445No Hit
TTCAAGTAATCCAGGATAGGC75050.12396846427271807No Hit
TGAGGTAGTAGTTTGTGCTGTT73680.12170548231330935No Hit
GACCCGCGGGCGCTCTCCAGTCCT73580.12154030114838901No Hit
ACTGGACTTGGAGTCAGAAGGCT73400.1212429750515324No Hit
CCCATAAAGTAGAAAGCACTA72350.11950857281986878No Hit
TCGTTTCCCGGGCGGCGCACCA71460.11803846045207772No Hit
CCCCCCACTGCTAAATTTGACTGA70500.11645272126884242No Hit
TGATGTAGTAGGTTGTATAGTT68280.1127856994076108No Hit
TTTAAACATCCTTGACTGGAAGCT67580.11162943125316838No Hit
CAACGGAATCCCAAAAGCAGCTGA67360.11126603269034363No Hit
ATCACATTGCCAGGGATTTCCA66990.11065486238013836No Hit
AGCAGCATTGTACAGGGCTATGT66420.10971332974009239No Hit
ATCACATTGCCAGGGATTTCC61660.10185070628988403No Hit
TTCACAGTGGCTAAGTTCTGC61100.10092569176633011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)