FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7784406
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG108119513.889242159260451No Hit
CAACGGAATCCCAAAAGCAGCTG5631657.234527592728333No Hit
AACATTCAACGCTGTCGGTGAGT4496435.776201806534757No Hit
CCCCCACTGCTAAATTTGACTG3129014.019587364790582No Hit
AACATTCAACGCTGTCGGTGAG2872293.6897998382920933No Hit
TAGCAGCACGTAAATATTGGCG2412473.099106084651803No Hit
AAGCTGCCAGTTGAAGAACTGT2377373.054015939045317No Hit
TTCAAGTAATCCAGGATAGGCT2158252.772530106985684No Hit
TATTGCACTTGTCCCGGCCTGTAA1497981.924334367965905No Hit
TAGCTTATCAGACTGATGTTGACT1255251.6125186687333628No Hit
TATTGCACTTGTCCCGGCCTGTAT1235131.5866721237304426No Hit
TAGCTTATCAGACTGATGTTGAC1196631.5372142717119328No Hit
TAGCTTATCAGACTGATGTTGA1090401.400749138726834No Hit
TGAGGTAGTAGGTTGTATAGTT848541.0900510584879566No Hit
TATTGCACTTGTCCCGGCCTGT748780.9618974138810333No Hit
TATTGCACTTGTCCCGGCCTGTA602750.7743044234845922No Hit
TGTAAACATCCTTGACTGGAAGCT602670.7742016539219563No Hit
TGAGGTAGTAGATTGTATAGTT558630.7176270096909129No Hit
CATAAAGTAGAAAGCACTACT494800.6356297449028224No Hit
AGCAGCATTGTACAGGGCTATGA451550.5800699501028082No Hit
TGTAAACATCCCCGACTGGAAGCT410100.5268224704621007No Hit
ACTGGACTTGGAGTCAGAAGGC377510.48495671988331546No Hit
TTCAAGTAATTCAGGATAGGTT346030.44451689698610275No Hit
CGCCTGTCTGAGCGTCGCT340050.43683487217907185No Hit
CACCCGGCTGTGTGCACATGTGC327640.4208927437751833No Hit
TTCTCACTACTGCACTTGACTAG322630.4144567999151123No Hit
TTAATGCTAATCGTGATAGGGGTT305570.39254119068301424No Hit
CATTGCACTTGTCTCGGTCTGA295700.37986199589281444No Hit
CCCATAAAGTAGAAAGCACTAC293030.37643206173984245No Hit
CTTCTCACTACTGCACTTGACTAG278050.35718846113627684No Hit
CCCATAAAGTAGAAAGCACT274520.3526537541849693No Hit
CAACGGAATCCCAAAAGCAGCTGT262380.3370584730549768No Hit
TGAGGTAGTAGTTTGTACAGTT248670.31944633925825555No Hit
AACATTCAACGCTGTCGGTGAGTT248210.3188554142730993No Hit
CAACACCAGTCGATGGGCTGT235670.30274628532992753No Hit
CTCCCACTGCTTCACTTGACTAG228440.2934584861067113No Hit
TATTGCACTTGTCCCGGCCTGTAG195980.25175973606720925No Hit
TGTCAGTTTGTCAAATACCCCAA189170.24301147704783127No Hit
AACATTCAACGCTGTCGGTGA184300.23675537992237303No Hit
TGTAAACATCCTACACTCTCAGCT184150.23656268699243074No Hit
ACCGGGTGCTGTAGGCTT175770.22579757530632397No Hit
ACCGGGTGCTGTAGGCTTA165700.2128614566095345No Hit
TGAGAACTGAATTCCATGGGTT165650.2127972256328871No Hit
ATCCCGGACGAGCCCCCA161070.2069136681719838No Hit
CCCCCCACTGCTAAATTTGACTGG159230.20454996823135893No Hit
TGAGGTAGTAGTTTGTGCTGTT158550.20367642694895408No Hit
ACCGGGTGCTGTAGGCTTT157260.20201926775145077No Hit
TAGCTTATCAGACTGATGTTGACA152900.19641832658779615No Hit
AACGGAATCCCAAAAGCAGCTG148170.19034207619695068No Hit
AACATTCAACGCTGTCGGTGAGA142700.18331520735172344No Hit
ACCCCCCACTGCTAAATTTGACTG142550.18312251442178118No Hit
CACTAGATTGTGAGCTCCTGGA136380.1751964119034901No Hit
TAGCAGCACGTAAATATTGGC134900.17329517499472663No Hit
CAAAGAATTCTCCTTTTGGGCT131900.1694413163958817No Hit
TATTGCACTTGTCCCGGCCTGTT124700.1601920557586539No Hit
CCCCCACTGCTAAATTTGACTGGCTT123310.15840643460785575No Hit
TGTAAACATCCCCGACTGGAAGC119120.15302387876480236No Hit
TGTCAGTTTGTCAAATACCCCA119030.15290826300683702No Hit
TTCAAGTAATCCAGGATAGGCTT118070.15167502825520662No Hit
CACTAGATTGTGAGCTCCTGGAA116120.14917002016595743No Hit
GACCCGCGGGCGCTCTCCAGTCCT113980.14642093436544804No Hit
ACTGGACTTGGAGTCAGAAGGCA113970.14640808817011858No Hit
ACTGGACTTGGAGTCAGAAGGCT112760.14485369853525112No Hit
TGAGGTAGTAGGTTGTGTGGTT111150.14278546108720433No Hit
CTACGCCTGTCTGAGCGTCGCT110500.14195045839078793No Hit
TTCACAGTGGCTAAGTTCCG107210.13772406012738803No Hit
CTCCCACTGCTTCACTTGACTAGCCT105960.13611828571120263No Hit
CCCCCCACTGCTAAATTTGACTGGCT104660.13444828031836983No Hit
TATTGCACTTGTCCCGGCCTGTAAT102830.1320974265730744No Hit
TTCAAGTAATCCAGGATAGGC98580.1266377935580441No Hit
TACGCCTGTCTGAGCGTCGCT98360.12635517726079548No Hit
ACTGGACTTGGAGTCAGAAGG97450.1251861734858125No Hit
AGCTCGGTCTGAGGCCCCTCAGT94740.12170485455152262No Hit
CAAAGAATTCTCCTTTTGGGCTT94470.12135800727762658No Hit
TGAGAACTGAATTCCATAGGCTGT90440.1161809905598449No Hit
CAAAGTGCTGTTCGTGCAGGT88810.11408706072113917No Hit
TTCACAGTGGCTAAGTTCCGC88420.11358605910328932No Hit
TATTGCACTTGTCCCGGCCTGTAAA88050.11311074987609844No Hit
ACCACAGGGTAGAACCACGGAC87990.11303367270412154No Hit
TTCACAGTGGCTAAGTTCTG86930.11167197599919634No Hit
TTATAAAGCAATGAGACTGATT84970.10915412171461766No Hit
AGCAGCATTGTACAGGGCTATG84670.10876873585473318No Hit
TCGTACGACTCTTAGCGG83580.10736850056381952No Hit
AGCAGCATTGTACAGGGCTATGT82850.10643072830476726No Hit
CCCATAAAGTAGAAAGCACTA81720.10497910823253566No Hit
TGAGGTAGTAGGTTGTATAGT80450.1033476414256913No Hit
CCCCCCACTGCTAAATTTGACTGT80160.1029751017611363No Hit
ATCACATTGCCAGGGATTTCC78030.10023886215595641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)