FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11197037
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG112116810.013077566859875No Hit
CAACGGAATCCCAAAAGCAGCTG9571098.547877442934233No Hit
AACATTCAACGCTGTCGGTGAGT4284133.826128287331729No Hit
CCCCCACTGCTAAATTTGACTG3726133.327782162370277No Hit
AAGCTGCCAGTTGAAGAACTGT3324692.969258742290483No Hit
TTCAAGTAATCCAGGATAGGCT3196882.8551124730587207No Hit
AACATTCAACGCTGTCGGTGAG3175372.835902033725529No Hit
TAGCAGCACGTAAATATTGGCG3069302.741171615312158No Hit
TATTGCACTTGTCCCGGCCTGTAA2024951.8084695084958635No Hit
TATTGCACTTGTCCCGGCCTGTAT1856691.658197610671466No Hit
TAGCTTATCAGACTGATGTTGAC1719411.5355937468099818No Hit
TAGCTTATCAGACTGATGTTGACT1554171.3880189910955907No Hit
TAGCTTATCAGACTGATGTTGA1442931.2886712797323077No Hit
TATTGCACTTGTCCCGGCCTGT1290851.1528496333449645No Hit
TCGTACGACTCTTAGCGG1261931.1270213718147042No Hit
CATAAAGTAGAAAGCACTACT967770.8643090131791116No Hit
TATTGCACTTGTCCCGGCCTGTA953670.8517163960429888No Hit
TGAGGTAGTAGGTTGTATAGTT852700.7615407540405555No Hit
TGTAAACATCCTTGACTGGAAGCT759380.6781972766545292No Hit
TTCAAGTAATTCAGGATAGGTT687210.6137427249726869No Hit
TGAGGTAGTAGATTGTATAGTT608090.5430811740641743No Hit
AGCAGCATTGTACAGGGCTATGA585440.5228526082391262No Hit
TTAATGCTAATCGTGATAGGGGTT555420.4960419439535656No Hit
CCCATAAAGTAGAAAGCACTAC527500.47110677583721483No Hit
CAACGGAATCCCAAAAGCAGCTGT510730.4561295992859539No Hit
TGTAAACATCCCCGACTGGAAGCT502710.44896699010640045No Hit
CAACGGAATCCCAAAAGCAGCT456060.4073041823475264No Hit
CCCATAAAGTAGAAAGCACT454920.40628605585566974No Hit
CATTGCACTTGTCTCGGTCTGA439520.3925324172814647No Hit
CACCCGGCTGTGTGCACATGTGC438380.39151429078960803No Hit
ACTGGACTTGGAGTCAGAAGGC342110.30553618783254893No Hit
ATCCCGGACGAGCCCCCA335200.29936491234243484No Hit
TTCTCACTACTGCACTTGACTAG333370.29773055139498067No Hit
CAACACCAGTCGATGGGCTGT316000.28221751879537416No Hit
GCCTGTCTGAGCGTCGCT286050.25546937104878725No Hit
CTTCTCACTACTGCACTTGACTAG277240.24760121807224536No Hit
TCGTTTCCCGGCCAATGCACCA273330.24410922282385958No Hit
TGAGGTAGTAGTTTGTACAGTT256860.22939997429677156No Hit
AACATTCAACGCTGTCGGTGAGTT255150.22787278455898646No Hit
TATTGCACTTGTCCCGGCCTGTAG247150.22072803724771115No Hit
CGCCTGTCTGAGCGTCGCT246230.21990639130691447No Hit
TGTAAACATCCTACACTCTCAGCT245170.21895971228817054No Hit
TGATGTAGTAGGTTGTATAGTT241890.21603036589054764No Hit
TGAGAACTGAATTCCATGGGTT237540.2121454095400417No Hit
CAAAGAATTCTCCTTTTGGGCT235280.21012701842460643No Hit
TACGCCTGTCTGAGCGTCGCT229030.2045451845876726No Hit
TAGCTTATCAGACTGATGTTGACA226420.20221421077736904No Hit
CTCCCACTGCTTCACTTGACTAG225470.2013657720341551No Hit
TATTGCACTTGTCCCGGCCTGTT225300.2012139461537905No Hit
ACCGGGTGCTGTAGGCTT224870.20082991598580946No Hit
CTACGCCTGTCTGAGCGTCGCT222460.19867756085828778No Hit
ACCGGGTGCTGTAGGCTTA211820.18917504693429163No Hit
ACCGGGTGCTGTAGGCTTT209940.18749603131614195No Hit
TAGCAGCACGTAAATATTGGC209030.18668331630948437No Hit
CACTAGATTGTGAGCTCCTGGA208820.1864957666925634No Hit
ACCCCCCACTGCTAAATTTGACTG208300.1860313581173305No Hit
CCCCCACTGCTAAATTTGACTGGCTT205610.18362893683391418No Hit
CGTTCGCGCTTTCCCCTG204240.18240539885685828No Hit
TGTCAGTTTGTCAAATACCCCAA183710.16407019106929807No Hit
AACGGAATCCCAAAAGCAGCTG182080.16261444880462572No Hit
CACTAGATTGTGAGCTCCTGGAA178740.15963151680216828No Hit
TCCTGTCTGAGCGTCGCT175920.15711299337494375No Hit
TGATGTAGTAGATTGTATAGTT169610.15147757393317535No Hit
CCCCCCACTGCTAAATTTGACTGG168870.1508166848068824No Hit
TTCAAGTAATCCAGGATAGGCTT165330.14765513412164308No Hit
CAAAGAATTCTCCTTTTGGGCTT164010.14647625081528265No Hit
TCGTTTCCCGGGCGGCGCACCA159410.14236802111129934No Hit
TGTAAACATCCCCGACTGGAAGC159340.1423055045723257No Hit
AGCTCGGTCTGAGGCCCCTCAGT157070.14027818252275134No Hit
TGAGGTAGTAGTTTGTGCTGTT156870.14009956383996947No Hit
CCCATAAAGTAGAAAGCACTA154350.13784896843691774No Hit
TTCAAGTAATCCAGGATAGGC153270.13688442754989555No Hit
GGAATACCGGGTGCTGTAGGCTTT148090.13225820366584481No Hit
AACATTCAACGCTGTCGGTGA147110.13138297212021358No Hit
TGTCAGTTTGTCAAATACCCCA143850.1284714875908689No Hit
TTCACAGTGGCTAAGTTCCGC142730.12747122296729035No Hit
TATTGCACTTGTCCCGGCCTGTAAT142460.12723008774553482No Hit
TATTGCACTTGTCCCGGCCTGTTT138650.12382740183853996No Hit
TTCACAGTGGCTAAGTTCCG137910.12316651271224699No Hit
AGCAGCATTGTACAGGGCTATGT137260.12258600199320589No Hit
TATTGCACTTGTCCCGGCCTGA136910.12227341929833759No Hit
TTATAAAGCAATGAGACTGATT133900.11958520812247025No Hit
CCCCCCACTGCTAAATTTGACTGGCT133390.11912973048137646No Hit
AACATTCAACGCTGTCGGTGAGA130490.11653975958103915No Hit
TGAGAACTGAATTCCATAGGCTGT129420.1155841496281561No Hit
TATTGCACTTGTCCCGGCCTGTAAA126930.11336034702752165No Hit
ACTTGACTTGGAGTCAGAAGGC117450.10489382146366044No Hit
ATCCCACCGCTGCCACCA116730.10425079420564565No Hit
TTCACAGTGGCTAAGTTCTGC116490.10403645178630741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)