FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7561990
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG85090511.252395202850044No Hit
CAACGGAATCCCAAAAGCAGCTG5492977.263921269401308No Hit
AACATTCAACGCTGTCGGTGAGT2929173.8735438687435453No Hit
CCCCCACTGCTAAATTTGACTG2566403.3938156490553415No Hit
AACATTCAACGCTGTCGGTGAG2394593.1666135501369346No Hit
TAGCAGCACGTAAATATTGGCG2041212.6993026967769067No Hit
AAGCTGCCAGTTGAAGAACTGT1943062.569508819768341No Hit
TTCAAGTAATCCAGGATAGGCT1845162.4400455435672357No Hit
GGAATACCGGGTGCTGTAGGCTTT1511591.998931498190291No Hit
TATTGCACTTGTCCCGGCCTGTAA1469371.9430996338265456No Hit
TATTGCACTTGTCCCGGCCTGTAT1413311.868965708761847No Hit
TAGCTTATCAGACTGATGTTGACT1062951.4056485131559284No Hit
TAGCTTATCAGACTGATGTTGAC1040301.3756960800001057No Hit
GGAATACCGGGTGCTGTAGGCTT977701.292913637812269No Hit
TATTGCACTTGTCCCGGCCTGT896991.1861824731320725No Hit
TATTGCACTTGTCCCGGCCTGTA867681.1474228344655308No Hit
TAGCTTATCAGACTGATGTTGA859871.1370948652404989No Hit
TGAATACCGGGTGCTGTAGGCTTT664510.8787501702594158No Hit
TGAGGTAGTAGGTTGTATAGTT580140.7671790097580135No Hit
CATAAAGTAGAAAGCACTACT575730.7613472115144294No Hit
TGTAAACATCCTTGACTGGAAGCT554170.7328361978791298No Hit
TCGTACGACTCTTAGCGG496680.6568112361957633No Hit
TTAATGCTAATCGTGATAGGGGTT477770.6318045911195334No Hit
AGCAGCATTGTACAGGGCTATGA435340.575695022077522No Hit
GCCTGTCTGAGCGTCGCT432060.5713575394836544No Hit
TGAATACCGGGTGCTGTAGGCTT428960.5672580894711577No Hit
TGAGGTAGTAGATTGTATAGTT387600.5125634918850726No Hit
TGTAAACATCCCCGACTGGAAGCT374280.49494908086363515No Hit
ATCCCGGACGAGCCCCCA357860.47323521982970096No Hit
CCCATAAAGTAGAAAGCACTAC352540.4662000346469646No Hit
TTCAAGTAATTCAGGATAGGTT307720.40692992188564125No Hit
CATTGCACTTGTCTCGGTCTGA273280.3613863546500326No Hit
CCCATAAAGTAGAAAGCACT270630.35788198609096283No Hit
ACTGGACTTGGAGTCAGAAGGC264960.35038395977778336No Hit
CAACGGAATCCCAAAAGCAGCTGT250910.3318041943985644No Hit
CAACACCAGTCGATGGGCTGT236840.31319798095474866No Hit
ACCGGGTGCTGTAGGCTTA226650.2997226920427031No Hit
GGAATACCGGGTGCTGTAGGCTTA226120.2990218183308891No Hit
CACCCGGCTGTGTGCACATGTGC222730.29453887138173945No Hit
CGCCTGTCTGAGCGTCGCT222040.29362641315315147No Hit
TATTGCACTTGTCCCGGCCTGTAG216490.28628707522755253No Hit
AACATTCAACGCTGTCGGTGAGTT215250.28464729522255383No Hit
TTCTCACTACTGCACTTGACTAG195110.25801409417362364No Hit
ACCGGGTGCTGTAGGCTTT187440.24787126140076884No Hit
CAAAGAATTCTCCTTTTGGGCT183270.24235683993234586No Hit
TATTGCACTTGTCCCGGCCTGTT182690.24158984605904002No Hit
ACCGGGTGCTGTAGGCTT179810.2377813247571076No Hit
TCCTGTCTGAGCGTCGCT170980.22610450423764117No Hit
TGTAAACATCCTACACTCTCAGCT169200.22375062648853014No Hit
CACTAGATTGTGAGCTCCTGGA168370.22265303180776488No Hit
ACCCCCCACTGCTAAATTTGACTG166200.21978341679901717No Hit
TGAGGTAGTAGTTTGTACAGTT162870.21537981404365783No Hit
TTCACAGTGGCTAAGTTCCG162440.21481118065482763No Hit
CTTCTCACTACTGCACTTGACTAG162120.21438801162127957No Hit
CACTAGATTGTGAGCTCCTGGAA161660.21377970613555425No Hit
CTCCCACTGCTTCACTTGACTAG152970.2022880220682651No Hit
AGCTCGGTCTGAGGCCCCTCAGT152470.2016268204533463No Hit
TGAGAACTGAATTCCATGGGTT143180.18934169444815452No Hit
TGTCAGTTTGTCAAATACCCCAA143110.18924912622206588No Hit
GGAATACCGGGTGCTGTAGGCT139410.18435623427166659No Hit
TAGCTTATCAGACTGATGTTGACA139370.18430333814247307No Hit
CCCCCACTGCTAAATTTGACTGGCTT124980.1652739556651093No Hit
CCCCCCACTGCTAAATTTGACTGG123790.16370029582160253No Hit
TAGCAGCACGTAAATATTGGC121630.1608439048451532No Hit
AACGGAATCCCAAAAGCAGCTG115910.15327975837048186No Hit
TGAGAACTGAATTCCATAGGCTGT115100.15220861175431333No Hit
CTACGCCTGTCTGAGCGTCGCT113420.1499869743281861No Hit
AACCGGGCGGAAACACCA112310.14851910674306631No Hit
TGATGTAGTAGGTTGTATAGTT112140.14829429819399392No Hit
CCCATAAAGTAGAAAGCACTA108900.14400971172931992No Hit
TCGTTTCCCGGGCGGCGCACCA108300.14321626979141733No Hit
TGAGGTAGTAGTTTGTGCTGTT107940.1427402046286758No Hit
TATTGCACTTGTCCCGGCCTGTTT103630.1370406467080755No Hit
TATTGCACTTGTCCCGGCCTGTAAA99930.13214775475767623No Hit
TGAATACCGGGTGCTGTAGGCTTA99570.13167168959493467No Hit
TATTGCACTTGTCCCGGCCTGTAAT98900.13078567943094344No Hit
CAACGGAATCCCAAAAGCAGCT97060.12835245748804217No Hit
CAAAGAATTCTCCTTTTGGGCTT95900.1268184697414305No Hit
TTCACAGTGGCTAAGTTCTG95650.12648786893397107No Hit
TTCAAGTAATCCAGGATAGGC94980.12560185876997987No Hit
AGCAGCATTGTACAGGGCTATGT94110.1244513679600211No Hit
TGTAAACATCCCCGACTGGAAGC93780.12401497489417468No Hit
ACTGGACTTGGAGTCAGAAGGCA91320.12076186294877407No Hit
TTCAAGTAATCCAGGATAGGCTT91240.12065607069038704No Hit
TGTCAGTTTGTCAAATACCCCA90660.1198890768170812No Hit
CCCCCCACTGCTAAATTTGACTGGCT90490.11966426826800881No Hit
AGCAGCATTGTACAGGGCTATG90030.1190559627822835No Hit
TATTGCACTTGTCCCGGCCTGA89320.11811705648909877No Hit
ATCCCACCGCTGCCACCA88700.11729716648659944No Hit
GACCCGCGGGCGCTCTCCAGTCCT88660.11724427035740591No Hit
TATTGCACTTGTCCCGGCCTG85530.11310514824801408No Hit
AACATTCAACGCTGTCGGTGAGA85390.1129200117958368No Hit
ACTGGACTTGGAGTCAGAAGG83730.11072482243430631No Hit
AGCTACATTGTCTGCTGGGTTT82810.10950821146285566No Hit
AACATTCAACGCTGTCGGTGA82760.1094420913013638No Hit
ACTGGACTTGGAGTCAGAAGGCT82230.10874121758954983No Hit
AGCTACATCTGGCTACTGGGTCTC81330.10755105468269596No Hit
CCCCCCACTGCTAAATTTGACTGA79840.10558067387023787No Hit
TTATAAAGCAATGAGACTGATT79650.10532941725656869No Hit
ACCACAGGGTAGAACCACGGAC76500.10116384708258011No Hit
TGATGTAGTAGATTGTATAGTT76170.10072745401673369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)