FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11655669
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG178222215.290602366968384No Hit
CAACGGAATCCCAAAAGCAGCTG8274707.099292198500145No Hit
AACATTCAACGCTGTCGGTGAGT5545164.757478957235317No Hit
CCCCCACTGCTAAATTTGACTG4942934.240794758327471No Hit
TTCAAGTAATCCAGGATAGGCT3751423.2185368338788622No Hit
AACATTCAACGCTGTCGGTGAG3583413.0743923836546836No Hit
AAGCTGCCAGTTGAAGAACTGT2728822.3411955161046527No Hit
TAGCAGCACGTAAATATTGGCG2676802.296564873281834No Hit
TATTGCACTTGTCCCGGCCTGTAA2401882.060696816287422No Hit
TATTGCACTTGTCCCGGCCTGTAT1961601.6829578808389292No Hit
TAGCTTATCAGACTGATGTTGACT1561531.3397171796831224No Hit
TAGCTTATCAGACTGATGTTGAC1242541.0660391951761843No Hit
TGAGGTAGTAGGTTGTATAGTT1179881.0122799472085215No Hit
TAGCTTATCAGACTGATGTTGA1104450.9475646571638231No Hit
TATTGCACTTGTCCCGGCCTGT986710.8465494344425876No Hit
TATTGCACTTGTCCCGGCCTGTA747970.6417220667470911No Hit
CGCCTGTCTGAGCGTCGCT728980.6254295656474116No Hit
TGTAAACATCCTTGACTGGAAGCT697100.5980780682773335No Hit
TGAGGTAGTAGATTGTATAGTT693480.5949722834442193No Hit
TGTAAACATCCCCGACTGGAAGCT630070.5405695717680383No Hit
CATAAAGTAGAAAGCACTACT557530.4783337618801632No Hit
ACTGGACTTGGAGTCAGAAGGC535180.45915854336632245No Hit
TTCTCACTACTGCACTTGACTAG530840.45543503337303076No Hit
CAACGGAATCCCAAAAGCAGCTGT506350.4344237984108849No Hit
CTTCTCACTACTGCACTTGACTAG482580.4140302886089164No Hit
CACCCGGCTGTGTGCACATGTGC473770.40647173491285654No Hit
CCCCCACTGCTAAATTTGACTGGCTT461450.39590177106093183No Hit
AGCAGCATTGTACAGGGCTATGA461180.3956701241258653No Hit
CTCCCACTGCTTCACTTGACTAG451710.38754532236630945No Hit
CCCATAAAGTAGAAAGCACTAC430020.3689363519159647No Hit
CCCATAAAGTAGAAAGCACT384210.32963358859967623No Hit
CCCCCCACTGCTAAATTTGACTGGCT379560.32564411360686374No Hit
AACATTCAACGCTGTCGGTGAGTT366280.3142505162080358No Hit
CCCCCCACTGCTAAATTTGACTGG362320.31085302782706No Hit
CAACGGAATCCCAAAAGCAGCT345990.2968426780135915No Hit
TTCAAGTAATTCAGGATAGGTT345170.29613915769227833No Hit
GACCCGCGGGCGCTCTCCAGTCCT337010.2891382725436009No Hit
ACCCCCCACTGCTAAATTTGACTG308690.26484108290995567No Hit
CAAAGAATTCTCCTTTTGGGCT283180.2429547373042251No Hit
TATTGCACTTGTCCCGGCCTGTAG268270.23016267877888436No Hit
CAACACCAGTCGATGGGCTGT255930.21957555589473243No Hit
TGAGGTAGTAGTTTGTACAGTT252060.21625528315877882No Hit
CATTGCACTTGTCTCGGTCTGA250110.21458227751663161No Hit
AGCTCGGTCTGAGGCCCCTCAGT237990.20418390398697833No Hit
AACATTCAACGCTGTCGGTGA222180.19061968901141582No Hit
CTCCCACTGCTTCACTTGACTAGCCT215360.18476845902195746No Hit
AACGGAATCCCAAAAGCAGCTG210040.1802041564495354No Hit
CTACGCCTGTCTGAGCGTCGCT209750.17995535048224173No Hit
TGTAAACATCCTACACTCTCAGCT209710.17992103241778742No Hit
TACGCCTGTCTGAGCGTCGCT203790.17484195887855086No Hit
GCCTGTCTGAGCGTCGCT203450.1745502553306893No Hit
CACTAGATTGTGAGCTCCTGGA194930.16724050760192316No Hit
TATTGCACTTGTCCCGGCCTGTT185940.15952752261581896No Hit
TGTAAACATCCCCGACTGGAAGC185500.15915002390682165No Hit
CACTAGATTGTGAGCTCCTGGAA184510.15830065181157768No Hit
TAGCTTATCAGACTGATGTTGACA181590.15579543310641372No Hit
AACATTCAACGCTGTCGGTGAGA179270.15380498536806422No Hit
CAAAGAATTCTCCTTTTGGGCTT176380.151325505211241No Hit
TGAGGTAGTAGTTTGTGCTGTT174470.1496868176335481No Hit
TTCAAGTAATCCAGGATAGGC170580.1463493858653673No Hit
TTCAAGTAATCCAGGATAGGCTT169220.1451825716739211No Hit
TGAGGTAGTAGGTTGTGTGGTT168640.1446849597393337No Hit
ACTGGACTTGGAGTCAGAAGGCT168490.14455626699763008No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC166730.14304627216164084No Hit
TATTGCACTTGTCCCGGCCTGTAAA165340.14185371942185387No Hit
TTCACAGTGGCTAAGTTCCG165120.1416649700673552No Hit
ATCCCGGACGAGCCCCCA161870.13887662733044323No Hit
TATTGCACTTGTCCCGGCCTGTAAT161440.1385077081375595No Hit
ACCGGGTGCTGTAGGCTT158910.13633709056082494No Hit
ACCGGGTGCTGTAGGCTTA158650.136114023141872No Hit
GGAATACCGGGTGCTGTAGGCTTT156460.13423510911299902No Hit
CAACGGAATCCCAAAAGCAGCTGA153630.1318071060528572No Hit
TTCACAGTGGCTAAGTTCTG150660.12925898976712533No Hit
CCCCCCACTGCTAAATTTGACTGT140200.1202848159123256No Hit
CAAAGTGCTGTTCGTGCAGGT139390.11958987510712599No Hit
CCCCCCACTGCTAAATTTGACTGTA138810.11909226317253863No Hit
ACTGGACTTGGAGTCAGAAGGCA138710.11900646801140287No Hit
TATTGCACTTGTCCCGGCCTGTTT138320.11867186688297342No Hit
ACCGGGTGCTGTAGGCTTT138130.1185088560768155No Hit
CCCCCACTGCTAAATTTGACTGGCT136250.11689590704746335No Hit
CCCCCCACTGCTAAATTTGACTGA136150.11681011188632759No Hit
TAGCAGCACGTAAATATTGGC134960.11578914946881212No Hit
ACGCCTGTCTGAGCGTCGCT130760.1121857527011105No Hit
TCGTACGACTCTTAGCGG129760.11132780108975297No Hit
GCGTTCGCGCTTTCCCCTG124040.10642031787278791No Hit
TTCACAGTGGCTAAGTTCTGC123350.10582833126095122No Hit
ACTGGACTTGGAGTCAGAAGG116920.10031170239992229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)