FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10245267
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG124990012.199779664112219No Hit
CCCCCCACTGCTAAATTTGACTG8882358.669710608810878No Hit
TTCAAGTAATCCAGGATAGGCT4566994.457658350924383No Hit
CCCCCACTGCTAAATTTGACTG3107743.0333421276380594No Hit
TAGCAGCACGTAAATATTGGCG1528731.4921329039057742No Hit
AACATTCAACGCTGTCGGTGAGT1345631.3134162340522701No Hit
AAGCTGCCAGTTGAAGAACTGT1241591.2118669040055277No Hit
CATAAAGTAGAAAGCACTACT1235861.2062740775813845No Hit
CGCCTGTCTGAGCGTCGCT931970.9096590650102141No Hit
CAACGGAATCCCAAAAGCAGCTGT876100.8551265672236751No Hit
ACTGGACTTGGAGTCAGAAGGC817120.7975585214128632No Hit
TAGCTTATCAGACTGATGTTGAC788770.769887207429538No Hit
CCCATAAAGTAGAAAGCACTAC776220.7576376486820695No Hit
TAGCTTATCAGACTGATGTTGA677000.6607929300427212No Hit
AACATTCAACGCTGTCGGTGAG652400.6367818427767671No Hit
AGCAGCATTGTACAGGGCTATGA640240.6249129476079052No Hit
CCCATAAAGTAGAAAGCACT584960.5709563254915659No Hit
AACCGGGCGGAAACACCA570880.5572133942434101No Hit
CTCCCACTGCTTCACTTGACTAG537670.5247984264343721No Hit
TTCAAGTAATTCAGGATAGGTT506380.494257494704628No Hit
CATTGCACTTGTCTCGGTCTGA505590.4934864069428352No Hit
GCCTGTCTGAGCGTCGCT477340.4659126990053065No Hit
CAACACCAGTCGATGGGCTGT472700.46138377848034606No Hit
TGTAAACATCCTACACTCTCAGCT471120.45984160295676046No Hit
CTACGCCTGTCTGAGCGTCGCT466600.45542980968675584No Hit
CCCCCACTGCTAAATTTGACTGGCTT456120.4452006960872762No Hit
ACCGGGTGCTGTAGGCTT419510.40946712272115504No Hit
CCCCCCACTGCTAAATTTGACTGGCT416670.4066951110205327No Hit
TACGCCTGTCTGAGCGTCGCT392770.38336726607515453No Hit
CAACGGAATCCCAAAAGCAGCT381620.37248419196883786No Hit
CCCCCCACTGCTAAATTTGACTGG352860.3444126931977468No Hit
ACCGGGTGCTGTAGGCTTT344220.3359795308409239No Hit
ACCGGGTGCTGTAGGCTTA290290.2833405903428383No Hit
CAAAGAATTCTCCTTTTGGGCT286610.2797486878575249No Hit
TTCAAGTAATCCAGGATAGGCTT277220.2705834801572277No Hit
TGTAAACATCCTACACTCAGCT275030.26844590775428306No Hit
TAGCTTATCAGACTGATGTTGACT271340.2648442446643899No Hit
ACGCCTGTCTGAGCGTCGCT267180.26078383315925296No Hit
AGCTCGGTCTGAGGCCCCTCAGT248900.2429414479876415No Hit
TCGTACGACTCTTAGCGG244920.23905672736493835No Hit
AACGGAATCCCAAAAGCAGCTG241850.23606022175898392No Hit
TTCACAGTGGCTAAGTTCCG235620.22997936510585817No Hit
CAAAGAATTCTCCTTTTGGGCTT222110.21679278831874269No Hit
CACTAGATTGTGAGCTCCTGGA212640.20754949578180834No Hit
CGCGACCTCAGATCAGACGT207590.20262039046908195No Hit
CACCCGGCTGTGTGCACATGTGC206250.20131246945540804No Hit
CTTCTCACTACTGCACTTGACTAG205840.20091228466764216No Hit
TGAGGTAGTAGGTTGTATAGTT199880.1950949643381671No Hit
TCCTGTCTGAGCGTCGCT197150.19243031928792093No Hit
ACTGGACTTGGAGTCAGAAGGCT188760.18424117204558943No Hit
TTCTCACTACTGCACTTGACTAG180400.1760813066169969No Hit
CACTAGATTGTGAGCTCCTGGAA168640.16460283563132128No Hit
ACTGGACTTGGAGTCAGAAGG168540.16450522958552471No Hit
GCGTTCGCGCTTTCCCCTG164480.16054242412618433No Hit
ACCACAGGGTAGAACCACGGAC158520.15472510379670926No Hit
ATCACATTGCCAGGGATTTCC158020.15423707356772645No Hit
CCCATAAAGTAGAAAGCACTA157420.15365143729294709No Hit
TTCACAGTGGCTAAGTTCCGC157160.15339766157387602No Hit
CAGTGCAATAGTATTGTCAAAGC154650.15094774982438233No Hit
TGAGGTAGTAGATTGTATAGTT151110.14749249580318405No Hit
CGCGACCTCAGATCAGACGTGGC149980.1463895474856829No Hit
TTCAAGTAATCCAGGATAGGC138770.13544790975188836No Hit
ACTGGACTTGGAGTCAGAAGGCA136220.13295895558407606No Hit
CCCCCACTGCTAAATTTGACTGGCT135960.132705179865005No Hit
AGCAGCATTGTACAGGGCTATGT134460.13124108917805655No Hit
GACCCGCGGGCGCTCTCCAGTCCT133850.13064569229869755No Hit
GGAATACCGGGTGCTGTAGGCTTT129840.12673168986225544No Hit
ATCCCGGACGAGCCCCCA126330.12330571765479612No Hit
TTCAAGTAATCCAGGATAGGCTA125830.1228176874258133No Hit
ATACCGGGTGCTGTAGGCTTA124760.1217733027357901No Hit
TATTGCACTTGTCCCGGCCTGT123840.12087532711446174No Hit
AACATTCAACGCTGTCGGTGAGTT123760.12079724227782448No Hit
CCCCCCACTGCTAAATTTGACTGTA121920.11900129103516775No Hit
CCAAGTCCTTCTGATCGAGGCCC119330.1164732944490368No Hit
TCCCACTGCTTCACTTGACTAG115630.11286187075456404No Hit
ATACCGGGTGCTGTAGGCTTT113730.11100735588442937No Hit
ATCACATTGCCAGGGATTTCCA113160.11045100142338896No Hit
CTCGACACAAGGGTTTGT112790.1100898590539417No Hit
ATCCCACCGCTGCCACCA111840.10916260161887435No Hit
ATAGGCGATAGAAATTGAAACCT109960.107327607957899No Hit
ATACCGGGTGCTGTAGGCTT109720.10709335344798725No Hit
CCTCGACACAAGGGTTTGT109710.1070835928434076No Hit
ACCCCCCACTGCTAAATTTGACTG105800.10326719645276206No Hit
CCCCCACTGCTAAATTTGACTGG103900.10141268158262737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)