FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8709796
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7691878.831286059972014No Hit
CCCCCCACTGCTAAATTTGACTG4583885.262901679901573No Hit
AAGCTGCCAGTTGAAGAACTGT3404923.909299368205639No Hit
TAGCAGCACGTAAATATTGGCG3169013.6384434262295007No Hit
AACATTCAACGCTGTCGGTGAGT3117793.579636078732498No Hit
TTCAAGTAATCCAGGATAGGCT2574772.9561771596028197No Hit
AACATTCAACGCTGTCGGTGAG1552301.7822461054196905No Hit
TAGCTTATCAGACTGATGTTGAC1499531.7216591525220568No Hit
CCCCCACTGCTAAATTTGACTG1274801.4636393320807972No Hit
TATTGCACTTGTCCCGGCCTGTAA1217851.3982531852640405No Hit
TATTGCACTTGTCCCGGCCTGTAT1147421.3173902121243712No Hit
TAGCTTATCAGACTGATGTTGACT1120531.2865169287547034No Hit
TATTGCACTTGTCCCGGCCTGT1015911.1663993048746493No Hit
TAGCTTATCAGACTGATGTTGA1006011.1550327929609372No Hit
TGAGGTAGTAGGTTGTATAGTT848950.9747070999137063No Hit
AGCAGCATTGTACAGGGCTATGA700980.8048179314417927No Hit
TGTAAACATCCTTGACTGGAAGCT694070.796884335752525No Hit
ACTGGACTTGGAGTCAGAAGGC597040.6854810376729834No Hit
TATTGCACTTGTCCCGGCCTGTA572710.6575469735456491No Hit
CAACGGAATCCCAAAAGCAGCTGT529020.607385063898167No Hit
TTAATGCTAATCGTGATAGGGGTT498800.5726884992484325No Hit
TTCAAGTAATTCAGGATAGGTT486780.5588879463996631No Hit
TGAGGTAGTAGATTGTATAGTT481130.5524009976812316No Hit
CTACGCCTGTCTGAGCGTCGCT480260.5514021223918448No Hit
TGTAAACATCCCCGACTGGAAGCT473010.5430781616469548No Hit
CGCCTGTCTGAGCGTCGCT471530.5413789255224807No Hit
CATAAAGTAGAAAGCACTACT460250.5284279907359484No Hit
CCCATAAAGTAGAAAGCACTAC397020.4558315717153421No Hit
TGTAAACATCCTACACTCTCAGCT357400.41034256141016395No Hit
CATTGCACTTGTCTCGGTCTGA296750.340708324282222No Hit
TACGCCTGTCTGAGCGTCGCT291730.3349446990492085No Hit
GGGCTACGCCTGTCTGAGCGTCGCT285990.3283544184042887No Hit
AACATTCAACGCTGTCGGTGAGTT262600.3014995988424987No Hit
TCGTACGACTCTTAGCGG252770.29021345620494443No Hit
CTCCCACTGCTTCACTTGACTAG244240.2804198858388876No Hit
GCCTGTCTGAGCGTCGCT243100.27911101477003597No Hit
GAATACCGGGTGCTGTAGGCTTT224750.25804278309159023No Hit
CCCATAAAGTAGAAAGCACT220030.2526235976135377No Hit
AGCTCGGTCTGAGGCCCCTCAGT211460.2427841019468194No Hit
GGAATACCGGGTGCTGTAGGCTTT206110.23664159298334886No Hit
CAACGGAATCCCAAAAGCAGCT194650.22348399434383998No Hit
GCGTTCGCGCTTTCCCCTG191280.21961478776311177No Hit
CACCCGGCTGTGTGCACATGTGC191020.2193162733088123No Hit
CCCCCACTGCTAAATTTGACTGGCTT188130.21599817033602164No Hit
TGAGGTAGTAGTTTGTACAGTT183880.21111860714074132No Hit
TGAGGTAGTAGTTTGTGCTGTT183180.21031491437916572No Hit
TAGCTTATCAGACTGATGTTGACA181860.20879937945733748No Hit
TGTCAGTTTGTCAAATACCCCAA175330.20130207412435377No Hit
ACCGGGTGCTGTAGGCTT173980.19975209522702944No Hit
AACGGAATCCCAAAAGCAGCTG173930.19969468860120262No Hit
TTCTCACTACTGCACTTGACTAG173040.1986728506614851No Hit
ACCGGGTGCTGTAGGCTTT170370.19560733684233247No Hit
TAGCAGCACGTAAATATTGGC169140.19419513384699252No Hit
ACGCCTGTCTGAGCGTCGCT168270.19319625855760572No Hit
ACTGGACTTGGAGTCAGAAGGCA166250.19087703087420188No Hit
CCCCCCACTGCTAAATTTGACTGGCT165910.19048666581857945No Hit
TATTGCACTTGTCCCGGCCTGTT164340.18868409776761705No Hit
GGAATACCGGGTGCTGTAGGCTT164320.18866113511728633No Hit
GAATACCGGGTGCTGTAGGCTT163490.187708185128561No Hit
CCCCCCACTGCTAAATTTGACTGG162730.1868356044159932No Hit
CTTCTCACTACTGCACTTGACTAG162070.18607783695507907No Hit
AACCGGGCGGAAACACCA161920.1859056170775986No Hit
CAAAGAATTCTCCTTTTGGGCT161380.18528562551866887No Hit
TGTAAACATCCTACACTCAGCT153830.17661722501881788No Hit
TAGCAGCACGTAAATATTGGCGT153330.17604315876054963No Hit
ACCGGGTGCTGTAGGCTTA153070.17574464430625011No Hit
CACTAGATTGTGAGCTCCTGGA152230.1747802129923594No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT151780.17426355335991794No Hit
TGTAAACATCCCCGACTGGAAGC150850.17319579011953898No Hit
TTCAAGTAATCCAGGATAGGCTT150670.1729891262665624No Hit
AGCAGCATTGTACAGGGCTATGT143410.16465368419650703No Hit
TATTGCACTTGTCCCGGCCTGTAG142920.16409109926340412No Hit
CAAAGAATTCTCCTTTTGGGCTT142400.1634940703548051No Hit
TCGTTTCCCGGGCGGCGCACCA136750.15700712163637356No Hit
TGAGGTAGTAGGTTGTGTGGTT135710.15581306381917556No Hit
CAACACCAGTCGATGGGCTGT132620.15226533434307762No Hit
TGTCAGTTTGTCAAATACCCCA130400.14971648015636646No Hit
ATCCCACCGCTGCCACCA128780.14785650547957727No Hit
GTCAGGATGGCCGAGCGGTCTAAGG126430.14515839406571635No Hit
GACCCGCGGGCGCTCTCCAGTCCT123980.1423454694002018No Hit
ACTGGACTTGGAGTCAGAAGGCT123330.14159918326445303No Hit
CACTAGATTGTGAGCTCCTGGAA122550.14070363990155452No Hit
ACCACAGGGTAGAACCACGGAC119370.1370525784989683No Hit
TCGTTTCCCGGCCAATGCACCA115040.13208116470236503No Hit
AGCAGCATTGTACAGGGCTATG114220.13113969603880504No Hit
ATCCCGGACGAGCCCCCA113240.1300145261725992No Hit
TTCACAGTGGCTAAGTTCCG112980.12971601171829972No Hit
TGAGAACTGAATTCCATGGGTT111830.12839565932428268No Hit
TTCAAGTAATCCAGGATAGGC110610.1269949376541081No Hit
ACTGGACTTGGAGTCAGAAGG109400.12560569730909887No Hit
CAAAGTGCTGTTCGTGCAGGTAG106550.12233351963696969No Hit
TTCACCACCTTCTCCACCCAGC103820.1191991178668249No Hit
ATCACATTGCCAGGGATTTCCA103550.11888912208736004No Hit
CTCCCACTGCTTCACTTGACTAGCCT101380.1163976745264757No Hit
CCAAGTCCTTCTGATCGAGGCCC99260.11396363359141821No Hit
CCTCGACACAAGGGTTTGT97250.11165588723317975No Hit
ACCCCCCACTGCTAAATTTGACTG96520.11081775049610806No Hit
AACATTCAACGCTGTCGGTGAGA96170.11041590411532028No Hit
TTATAAAGCAATGAGACTGATT95310.10942851015109883No Hit
ATCACATTGCCAGGGATTTCC95070.10915295834713006No Hit
TTCACAGTGGCTAAGTTCCGC94470.10846407883720814No Hit
TATTGCACTTGTCCCGGCCTGTTT92860.10661558548558428No Hit
TATTGCACTTGTCCCGGCCTGA92820.10656966018492281No Hit
AACATTCAACGCTGTCGGTGA92470.10616781380413502No Hit
TGATGTAGTAGGTTGTATAGTT89690.10297600540816342No Hit
TAGCAGCACATCATGGTTTACA89050.10224120059758No Hit
TATTGCACTTGTCCCGGCCTGTAAT88720.10186231686712295No Hit
TTCACAGTGGCTAAGTTCTGC87190.10010567411682203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)