FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134662
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4332267.061937560700166No Hit
CCCCCCACTGCTAAATTTGACTG4128596.729938829555728No Hit
AACATTCAACGCTGTCGGTGAGT2057063.353175773987222No Hit
AAGCTGCCAGTTGAAGAACTGT1884723.0722475011663235No Hit
TAGCAGCACGTAAATATTGGCG1540312.510831077572No Hit
TAGCTTATCAGACTGATGTTGAC1331792.1709264503896057No Hit
TTCAAGTAATCCAGGATAGGCT1290732.103995297540435No Hit
AACATTCAACGCTGTCGGTGAG1204821.9639549823608864No Hit
CCCCCACTGCTAAATTTGACTG1163991.897398748292897No Hit
TAGCTTATCAGACTGATGTTGACT884081.4411225915951034No Hit
TATTGCACTTGTCCCGGCCTGTAA788631.2855312974048123No Hit
CGCCTGTCTGAGCGTCGCT716221.1674970845989558No Hit
TATTGCACTTGTCCCGGCCTGTAT693841.131015857108346No Hit
TGAGGTAGTAGGTTGTATAGTT669801.0918286940666007No Hit
TAGCTTATCAGACTGATGTTGA666551.0865309286803413No Hit
AGCAGCATTGTACAGGGCTATGA625121.0189966456179655No Hit
TATTGCACTTGTCCCGGCCTGT567270.9246964217425508No Hit
TGTAAACATCCTTGACTGGAAGCT528470.8614492534389017No Hit
ACTGGACTTGGAGTCAGAAGGC414380.6754732371563421No Hit
TATTGCACTTGTCCCGGCCTGTA400940.6535649396820884No Hit
TGAGGTAGTAGATTGTATAGTT389930.6356177406350993No Hit
TTAATGCTAATCGTGATAGGGGTT365080.5951102114509325No Hit
TGTAAACATCCCCGACTGGAAGCT304810.49686518996482615No Hit
TTCAAGTAATTCAGGATAGGTT284080.46307359720877855No Hit
ACCGGGTGCTGTAGGCTT257590.41989273410662237No Hit
TACGCCTGTCTGAGCGTCGCT257130.4191428965442595No Hit
CATAAAGTAGAAAGCACTACT254800.41534480628272596No Hit
CTACGCCTGTCTGAGCGTCGCT251960.41071537437596395No Hit
CAACGGAATCCCAAAAGCAGCTGT248910.4057436253211668No Hit
GCGTTCGCGCTTTCCCCTG231510.3773802044839634No Hit
GCCTGTCTGAGCGTCGCT216870.3535158090209371No Hit
ACCGGGTGCTGTAGGCTTT213110.3473867019894495No Hit
AACATTCAACGCTGTCGGTGAGTT202690.33040125112027363No Hit
CCCATAAAGTAGAAAGCACTAC185290.3020378302830702No Hit
CATTGCACTTGTCTCGGTCTGA176730.28808433129649197No Hit
TGTAAACATCCTACACTCTCAGCT169150.275728312334078No Hit
CAACGGAATCCCAAAAGCAGCT166280.27104997797759683No Hit
ACGCCTGTCTGAGCGTCGCT161770.26369830970312624No Hit
TAGCTTATCAGACTGATGTTGACA160010.2608293659862597No Hit
TGAGGTAGTAGTTTGTACAGTT156870.25571090958230464No Hit
ACCGGGTGCTGTAGGCTTA151460.2468921678162546No Hit
CAACACCAGTCGATGGGCTGT137510.22415252869677255No Hit
TCGTACGACTCTTAGCGG136100.22185411355996465No Hit
ACTGGACTTGGAGTCAGAAGGCA133330.21733878736921447No Hit
TGAGGTAGTAGTTTGTGCTGTT130130.21212252606582072No Hit
CTCCCACTGCTTCACTTGACTAG124720.2033037842997707No Hit
AGCTCGGTCTGAGGCCCCTCAGT121220.19759849849918382No Hit
CCCCCACTGCTAAATTTGACTGGCTT121040.1973050838008679No Hit
AACCGGGCGGAAACACCA120130.19582170949271532No Hit
CCCCCCACTGCTAAATTTGACTGG110860.1807108525294466No Hit
CCCATAAAGTAGAAAGCACT109160.1779397137120187No Hit
AGCAGCATTGTACAGGGCTATGT108890.17749959166454485No Hit
AACGGAATCCCAAAAGCAGCTG108750.17727138023252137No Hit
GGAATACCGGGTGCTGTAGGCTTT104840.17089776095243714No Hit
CCAAGTCCTTCTGATCGAGGCCC103240.1682896303007403No Hit
CACCCGGCTGTGTGCACATGTGC103100.1680614188687168No Hit
GAATACCGGGTGCTGTAGGCTTT101130.16485015800381506No Hit
ACTGGACTTGGAGTCAGAAGG97010.1581342215756956No Hit
CCCCCCACTGCTAAATTTGACTGGCT96720.15766149789507555No Hit
CACTAGATTGTGAGCTCCTGGA96160.15674865216698167No Hit
TATTGCACTTGTCCCGGCCTGTT94880.15466214764562417No Hit
TATTGCACTTGTCCCGGCCTGTAG94450.15396121253298062No Hit
TGAGGTAGTAGGTTGTGTGGTT92790.15125527698184513No Hit
AGCAGCATTGTACAGGGCTATG92620.15097816310010234No Hit
TTCACAGTGGCTAAGTTCCG92400.150619545135494No Hit
TTCAAGTAATCCAGGATAGGCTT90740.14791360958435854No Hit
ACCACAGGGTAGAACCACGGAC89960.1466421458916563No Hit
TAGCAGCACGTAAATATTGGC89490.14587600751272034No Hit
CCTCGACACAAGGGTTTGT89440.1457945034298548No Hit
CAAAGAATTCTCCTTTTGGGCT89170.14535438138238096No Hit
TTATAAAGCAATGAGACTGATT88990.14506096668406507No Hit
TGTCAGTTTGTCAAATACCCCAA86050.1402685266115721No Hit
CACTAGATTGTGAGCTCCTGGAA84720.13810051800734907No Hit
TTCTCACTACTGCACTTGACTAG84680.13803531474105663No Hit
CTTCTCACTACTGCACTTGACTAG84550.13782340412560626No Hit
GGAATACCGGGTGCTGTAGGCTT84500.13774190004274073No Hit
GGGCTACGCCTGTCTGAGCGTCGCT83940.13682905431464684No Hit
CAAAGTGCTGTTCGTGCAGGTAG81410.13270494772165117No Hit
CTGCGTTCGCGCTTTCCCCTG79020.12880905256067898No Hit
TCGTTCGCGCTTTCCCCTG78950.12869494684466723No Hit
GAATACCGGGTGCTGTAGGCTT77840.12688555620505254No Hit
CAAAGAATTCTCCTTTTGGGCTT77730.12670624722274837No Hit
ATCCCGGACGAGCCCCCA76440.12460344188481777No Hit
TGATGTAGTAGGTTGTATAGTT72580.11831132668759908No Hit
ATCCCACCGCTGCCACCA70570.1150348625564049No Hit
TGTAAACATCCCCGACTGGAAGC70340.11465994377522347No Hit
TGTCAGTTTGTCAAATACCCCA70140.11433392744376136No Hit
ACTGGACTTGGAGTCAGAAGGCT69370.11307876456763226No Hit
TAGCAGCACGTAAATATTGGCGT68970.11242673190470803No Hit
TCCTGTCTGAGCGTCGCT68880.11228002455555008No Hit
TGAGGTAGTAGGTTGTATAGT67460.10996530860216912No Hit
ACCCCCCACTGCTAAATTTGACTG66820.10892205634149037No Hit
TGTAAACATCCTACACTCAGCT65040.1060205109914776No Hit
TCGTTTCCCGGCCAATGCACCA63780.10396660810326633No Hit
CAAGTCCTTCTGATCGAGGCCC63130.10290705502601447No Hit
AACATTCAACGCTGTCGGTGAGA62210.10140737990128879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)