FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9823857
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9110249.273587756825043No Hit
CCCCCCACTGCTAAATTTGACTG6921667.045766240286275No Hit
TTCAAGTAATCCAGGATAGGCT3375093.435605791085925No Hit
TAGCAGCACGTAAATATTGGCG3199553.2569183366573844No Hit
AACATTCAACGCTGTCGGTGAGT3081273.136517561279648No Hit
AAGCTGCCAGTTGAAGAACTGT2726072.7749487803008535No Hit
AACATTCAACGCTGTCGGTGAG1994162.0299155413194634No Hit
TAGCTTATCAGACTGATGTTGACT1978362.0138322453187176No Hit
TATTGCACTTGTCCCGGCCTGTAA1898031.9320619182465706No Hit
TATTGCACTTGTCCCGGCCTGTAT1832421.86527552263841No Hit
CCCCCACTGCTAAATTTGACTG1812651.8451510440349448No Hit
TAGCTTATCAGACTGATGTTGAC1795581.8277749767733793No Hit
TAGCTTATCAGACTGATGTTGA1278191.3011081085565475No Hit
TGAGGTAGTAGGTTGTATAGTT1086331.1058080344614136No Hit
TATTGCACTTGTCCCGGCCTGT936920.9537190942417016No Hit
TGTAAACATCCTTGACTGGAAGCT873490.8891517863095931No Hit
TATTGCACTTGTCCCGGCCTGTA801340.8157081276732753No Hit
CATAAAGTAGAAAGCACTACT773040.7869007050896609No Hit
TGAGGTAGTAGATTGTATAGTT710610.7233513272841817No Hit
AGCAGCATTGTACAGGGCTATGA706480.719147275861202No Hit
CCCATAAAGTAGAAAGCACTAC658970.6707854155450349No Hit
ACTGGACTTGGAGTCAGAAGGC642300.6538165203341213No Hit
CAACGGAATCCCAAAAGCAGCTGT576100.5864295459512491No Hit
TGTAAACATCCCCGACTGGAAGCT575840.5861648841183253No Hit
TTCAAGTAATTCAGGATAGGTT569330.5795381589939674No Hit
CCCCCACTGCTAAATTTGACTGGCTT468200.47659488528792715No Hit
CTCCCACTGCTTCACTTGACTAG405490.41276048704699186No Hit
GGAATACCGGGTGCTGTAGGCTTT377820.3845943604431538No Hit
CAAAGAATTCTCCTTTTGGGCT375550.3822836590557049No Hit
TGTAAACATCCTACACTCTCAGCT364680.3712187585792423No Hit
CCCCCCACTGCTAAATTTGACTGGCT329970.33588640388393276No Hit
CATTGCACTTGTCTCGGTCTGA326820.33267992398504986No Hit
CCCATAAAGTAGAAAGCACT299050.3044120043685489No Hit
CACTAGATTGTGAGCTCCTGGAA282610.28767723308676013No Hit
TAGCTTATCAGACTGATGTTGACA279860.284877925238529No Hit
CACTAGATTGTGAGCTCCTGGA271040.2758997815216569No Hit
TAGCAGCACATCATGGTTTACA261410.26609711440221495No Hit
AGCTCGGTCTGAGGCCCCTCAGT257350.2619643180880992No Hit
TGAGGTAGTAGTTTGTACAGTT256830.2614349944222519No Hit
GGAATACCGGGTGCTGTAGGCTT251500.2560094268473167No Hit
AACATTCAACGCTGTCGGTGAGTT248960.2534238843256778No Hit
CACCCGGCTGTGTGCACATGTGC237140.2413919502289172No Hit
CTTCTCACTACTGCACTTGACTAG234720.23892855932247387No Hit
CAACACCAGTCGATGGGCTGT221230.22519668191424203No Hit
TATTGCACTTGTCCCGGCCTGTAG219270.2232015388660482No Hit
TTCTCACTACTGCACTTGACTAG218770.2226925738027335No Hit
GTCAGGATGGCCGAGCGGTCTAAGG217160.22105370629886004No Hit
TTCACAGTGGCTAAGTTCCG214210.218050812425303No Hit
CTACGCCTGTCTGAGCGTCGCT212090.2158928005568485No Hit
CTCCCACTGCTTCACTTGACTAGCCT211250.2150377392504797No Hit
ACTGGACTTGGAGTCAGAAGGCA209460.21321564432381293No Hit
CAAAGAATTCTCCTTTTGGGCTT207680.21140372869841245No Hit
TATTGCACTTGTCCCGGCCTGTT201250.2048584379841848No Hit
CCCCCCACTGCTAAATTTGACTGG193330.19679643138127925No Hit
TAGCAGCACGTAAATATTGGC192310.1957581426521172No Hit
TGAGGTAGTAGTTTGTGCTGTT191940.19538150850526426No Hit
TTCACAGTGGCTAAGTTCCGC191850.1952898947938676No Hit
TGTCAGTTTGTCAAATACCCCAA191580.19501505365967767No Hit
AACGGAATCCCAAAAGCAGCTG191400.19483182623688436No Hit
ATCACATTGCCAGGGATTTCC187840.19120799498608337No Hit
ACTGGACTTGGAGTCAGAAGGCT181250.18449983545159504No Hit
TTCAAGTAATCCAGGATAGGCTT177560.18074367328433222No Hit
CGCCTGTCTGAGCGTCGCT176520.17968502595263755No Hit
ATCCCGGACGAGCCCCCA173040.1761426291119669No Hit
TATTGCACTTGTCCCGGCCTGTTT168330.17134817821554202No Hit
ATCACATTGCCAGGGATTTCCA163080.1660040450507372No Hit
TGTAAACATCCCCGACTGGAAGC162200.16510826653930324No Hit
TATTGCACTTGTCCCGGCCTGTAAT156190.15899050647826002No Hit
ACCCCCCACTGCTAAATTTGACTG155670.1584611828124127No Hit
AGCAGCATTGTACAGGGCTATGT148070.15072491385002856No Hit
ACCGGGTGCTGTAGGCTTA145830.1484447503663785No Hit
TGTCAGTTTGTCAAATACCCCA145600.14821062643725372No Hit
TGTAAACATCCTACACTCAGCT145500.14810883342459077No Hit
TATTGCACTTGTCCCGGCCTGTAAA141220.14375209248261656No Hit
CCCATAAAGTAGAAAGCACTA140370.14288685187498149No Hit
TTCAAGTAATCCAGGATAGGC139990.1425000384268623No Hit
ACTGGACTTGGAGTCAGAAGG137650.14011808193054928No Hit
TCAGGCTCAGTCCCCTCCCGAT135800.13823491119628473No Hit
TTCACAGTGGCTAAGTTCTGC134240.1366469401987427No Hit
CAACGGAATCCCAAAAGCAGCT132000.13436677671509265No Hit
TTAATGCTAATCGTGATAGGGGTT123160.12536827439568798No Hit
TCGTTTCCCGGGCGGCGCACCA123070.1252766606842913No Hit
ATGCACCTGGGCAAGGATTCTGA117850.11996306542328539No Hit
GCCTGTCTGAGCGTCGCT115130.11719429547885317No Hit
TAGCAGCACGTAAATATTGGCGT113180.11520933173192567No Hit
GACCCGCGGGCGCTCTCCAGTCCT111940.11394709837490508No Hit
TACGCCTGTCTGAGCGTCGCT109330.11129030074440213No Hit
CAAAGTGCTGTTCGTGCAGGTAG108430.11037416363043559No Hit
ACCGGGTGCTGTAGGCTT107430.10935623350380608No Hit
TTATAAAGCAATGAGACTGATT106510.10841973778730697No Hit
TGAGGTAGTAGGTTGTGTGGTT106290.10819579315944847No Hit
ACCGGGTGCTGTAGGCTTT106050.1079514899290574No Hit
TTCACAGTGGCTAAGTTCTG104030.10589527107326582No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC102020.10384923151874055No Hit
ACCACAGGGTAGAACCACGGAC101480.10329954925036064No Hit
CCCCCACTGCTAAATTTGACTGGCT101370.10318757693643138No Hit
TGAGGTAGTAGGTTGTATAGT99170.10094813065784651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)