FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11002258
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAC4797424.360395838745101No Hit
AAGCTGCCAGTTGAAGAACTGT4484394.075881514503659No Hit
TTCAAGTAATCCAGGATAGGCT4114013.739241526602994No Hit
CAACGGAATCCCAAAAGCAGCTG4002043.637471508121333No Hit
TAGCAGCACGTAAATATTGGCG3788823.443674925637992No Hit
CCCCCCACTGCTAAATTTGACTG3772633.428959764441081No Hit
AACATTCAACGCTGTCGGTGAGT3739623.398956832315694No Hit
TAGCTTATCAGACTGATGTTGA3610933.2819899333391382No Hit
TAGCTTATCAGACTGATGTTGACT3091622.809986822704939No Hit
TGAGGTAGTAGGTTGTATAGTT2466402.241721653864143No Hit
TGAGGTAGTAGATTGTATAGTT2422532.2018480206517608No Hit
AACATTCAACGCTGTCGGTGAG2389592.1719087118298805No Hit
CATAAAGTAGAAAGCACTACT1620401.4727885857612137No Hit
TGTAAACATCCTTGACTGGAAGCT1536921.3969132518070382No Hit
CCCCCACTGCTAAATTTGACTG1374231.2490436054126344No Hit
TTCAAGTAATTCAGGATAGGTT1305581.1866473227586556No Hit
TATTGCACTTGTCCCGGCCTGTAA1153261.0482030143266956No Hit
GCCTGTCTGAGCGTCGCT1136411.0328879762681442No Hit
CGCCTGTCTGAGCGTCGCT1112031.0107288885608754No Hit
TATTGCACTTGTCCCGGCCTGTAT1096080.9962318644045614No Hit
TATTGCACTTGTCCCGGCCTGT882440.8020535421001762No Hit
TGAGGTAGTAGTTTGTACAGTT874980.7952731157549661No Hit
AGCAGCATTGTACAGGGCTATGA869890.7906467926856469No Hit
TTATAAAGCAATGAGACTGATT623560.5667563876433365No Hit
TGTCAGTTTGTCAAATACCCCAA591430.53755329133347No Hit
TATTGCACTTGTCCCGGCCTGTA568360.5165848683061242No Hit
ACTGGACTTGGAGTCAGAAGGC548030.49810684315892245No Hit
CAACACCAGTCGATGGGCTGT547990.4980704869854897No Hit
TGTAAACATCCCCGACTGGAAGCT539540.4903902453478186No Hit
TGAGGTAGTAGTTTGTGCTGTT456610.4150148087783435No Hit
CATTGCACTTGTCTCGGTCTGA419950.38169437582721655No Hit
TAGCTTATCAGACTGATGTTGACA399380.36299821363941837No Hit
TGTAAACATCCTACACTCTCAGCT377390.34301140729475715No Hit
TTCACAGTGGCTAAGTTCCG375750.34152080418401387No Hit
TACGCCTGTCTGAGCGTCGCT341770.310636234852882No Hit
TGTCAGTTTGTCAAATACCCCA341140.310063625121316No Hit
AACCGGGCGGAAACACCA336020.3054100349219224No Hit
AACATTCAACGCTGTCGGTGAGTT308800.28066965890092743No Hit
TAGCTTATCAGACTGATGTTG308590.2804787889904054No Hit
TTCACAGTGGCTAAGTTCCGC301880.27438004089705953No Hit
TGTAAACATCCTACACTCAGCT294480.26765414881199845No Hit
TTCAAGTAATCCAGGATAGGCTT287460.2612736403745486No Hit
GCGTTCGCGCTTTCCCCTG282070.25637464600448384No Hit
TAGCAGCACGTAAATATTGGC248290.22567185754051575No Hit
TGAGGTAGTAAGTTGTATTGTT247000.22449937094730915No Hit
TGAGGTAGTAGGTTGTATAGT239160.21737356095448773No Hit
TCGTACGACTCTTAGCGG236820.21524672480867107No Hit
CCCATAAAGTAGAAAGCACTAC234250.21291084066561608No Hit
TAGCAGCACGTAAATATTGGCGT233810.2125109227578557No Hit
TCGACCGGACCTCGACCGGCT232050.21091125112681414No Hit
TGAGGTAGTAGATTGTATAGT229520.2086117231571919No Hit
TTCTCACTACTGCACTTGACTAG228760.2079209558619694No Hit
CAACGGAATCCCAAAAGCAGCTGT225920.2053396675482433No Hit
ACGCCTGTCTGAGCGTCGCT201410.18306242227731798No Hit
AGCAGCATTGTACAGGGCTATGT200220.18198082611769328No Hit
TTCACAGTGGCTAAGTTCTG191910.17442783108703686No Hit
CTACGCCTGTCTGAGCGTCGCT189380.17212830311741462No Hit
CAAAGAATTCTCCTTTTGGGCTT189370.17211921407405642No Hit
CAAAGAATTCTCCTTTTGGGCT189260.17201923459711632No Hit
TAGCAGCACATAATGGTTTGT180820.16434808200280343No Hit
CAACGGAATCCCAAAAGCAGCT180430.163993609311834No Hit
TTCAAGTAATCCAGGATAGGC173490.1576858132212497No Hit
TTCACAGTGGCTAAGTTCTGC173160.1573858747904294No Hit
TCCTGTCTGAGCGTCGCT170490.15495910021379247No Hit
TGTCAGTTTGTCAAATACC163770.14885126307708837No Hit
ACTGGACTTGGAGTCAGAAGG162200.14742428326985244No Hit
ACTGGACTTGGAGTCAGAAGGCA159570.14503386486664827No Hit
TGTAAACATCCCCGACTGGAAGC156530.14227079568575832No Hit
TTATAAAGCAATGAGACTGAT156300.14206174768851995No Hit
TGTCAGTTTGTCAAATACCCC154790.14068930214143316No Hit
AACATTCAACGCTGTCGGTGA154460.14038936371061286No Hit
CTTCTCACTACTGCACTTGACTAG154390.14032574040710552No Hit
AGCAGCATTGTACAGGGCTATG148670.13512680760622045No Hit
TATTGCACTTGTCCCGGCCTGTT148470.13494502673905667No Hit
TGTAGTGTTTCCTACTTTATGGA147970.13449057457114713No Hit
TGTAAACATCCTTGACTGGAAGC146190.1328727248533892No Hit
AACGGAATCCCAAAAGCAGCTG139040.12637405885228287No Hit
CACCCGGCTGTGTGCACATGTGC136330.1239109281022132No Hit
CAAAGTGCTGTTCGTGCAGGT134480.12222945508094793No Hit
TCGTTTCCCGGCCAATGCACCA133700.12152050969900907No Hit
AGAGGTAGTAGGTTGCATAGTT133300.12115694796468142No Hit
CTCCCACTGCTTCACTTGACTAG130820.11890286521185015No Hit
TGAGGTAGTAGGTTGTGTGGTT130220.11835752261035871No Hit
TATTGCACTTGTCCCGGCCTGTAG125500.11406749414529273No Hit
AGTGTTTCCTACTTTATGGATG122860.1116679866987304No Hit
ACCACAGGGTAGAACCACGGAC119800.10888673943112405No Hit
CAACACCAGTCGATGGGCTGTC118210.10744158153717175No Hit
TGAGGTAGTAGGTTGTATAGTTT118160.10739613632038078No Hit
TGTCAGTTTGTCAAATACCC118060.10730524588679886No Hit
TTCACAGTGGCTAAGTTCCGCT117560.10685079371888934No Hit
ACTGGACTTGGAGTCAGAAGGCT117100.10643269772441256No Hit
ATCACATTGCCAGGGATTTCC116540.10592371129635389No Hit
ACCGGGTGCTGTAGGCTT114950.10447855340240159No Hit
CAAAGTGCTGTTCGTGCAGGTAG114920.104451286272327No Hit
AACATTCAACGCTGTCGGTGAGA112030.1018245527418099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)