FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8833968
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG105615411.9556013786783No Hit
CCCCCCACTGCTAAATTTGACTG88736310.044897151540507No Hit
AACATTCAACGCTGTCGGTGAGT3283343.716721636302056No Hit
TTCAAGTAATCCAGGATAGGCT2891483.273138412998553No Hit
AACATTCAACGCTGTCGGTGAG2160942.4461714147028832No Hit
TAGCAGCACGTAAATATTGGCG2035932.3046608273881No Hit
CCCCCACTGCTAAATTTGACTG1920292.1737570251556266No Hit
AAGCTGCCAGTTGAAGAACTGT1850172.094381596129848No Hit
TATTGCACTTGTCCCGGCCTGTAA1275811.4442094424611907No Hit
TATTGCACTTGTCCCGGCCTGTAT1191181.3484087784787084No Hit
TAGCTTATCAGACTGATGTTGAC1166531.3205051229526754No Hit
TAGCTTATCAGACTGATGTTGACT1016601.1507852416943325No Hit
TAGCTTATCAGACTGATGTTGA855440.9683530662551642No Hit
CAACGGAATCCCAAAAGCAGCTGT662450.7498895173720348No Hit
AGCAGCATTGTACAGGGCTATGA628290.711220597584234No Hit
CCCATAAAGTAGAAAGCACTAC596310.675019425019425No Hit
TATTGCACTTGTCCCGGCCTGT552320.6252230028453805No Hit
ACTGGACTTGGAGTCAGAAGGC504140.5706835252289798No Hit
TATTGCACTTGTCCCGGCCTGTA476000.5388292101578815No Hit
TGAGGTAGTAGGTTGTATAGTT452480.5122047080089038No Hit
CATAAAGTAGAAAGCACTACT417300.472381154199336No Hit
TTCAAGTAATTCAGGATAGGTT415300.4701171659213617No Hit
CCCCCACTGCTAAATTTGACTGGCTT402720.4558766796529034No Hit
CAACGGAATCCCAAAAGCAGCT397060.44946959282623616No Hit
CTCCCACTGCTTCACTTGACTAG375250.4247808006549265No Hit
TCGTACGACTCTTAGCGG372640.42182629595217014No Hit
TGTAAACATCCTTGACTGGAAGCT360970.40861592435019006No Hit
CAAAGAATTCTCCTTTTGGGCT338030.38264797880182494No Hit
TGAGGTAGTAGATTGTATAGTT329520.37301470867904435No Hit
TTAATGCTAATCGTGATAGGGGTT318330.36034769426377816No Hit
CCCCCCACTGCTAAATTTGACTGGCT316340.3580950259271938No Hit
TGTAAACATCCCCGACTGGAAGCT288260.3263086305044347No Hit
CCCATAAAGTAGAAAGCACT275470.31183042546678913No Hit
CATTGCACTTGTCTCGGTCTGA275430.3117851457012296No Hit
CACCCGGCTGTGTGCACATGTGC273960.3101211143169185No Hit
TGTAAACATCCTACACTCTCAGCT264020.2988690925753863No Hit
AACCGGGCGGAAACACCA252670.28602095909788217No Hit
AGCTCGGTCTGAGGCCCCTCAGT246160.2786516772530759No Hit
AACATTCAACGCTGTCGGTGAGTT246090.2785724376633468No Hit
CTTCTCACTACTGCACTTGACTAG223020.2524573328769133No Hit
CAAAGAATTCTCCTTTTGGGCTT217930.24669548270946873No Hit
CCCCCCACTGCTAAATTTGACTGG209380.23701693282112862No Hit
ACCCCCCACTGCTAAATTTGACTG205560.23269271521019771No Hit
CTCCCACTGCTTCACTTGACTAGCCT196290.2221991295417869No Hit
TTCTCACTACTGCACTTGACTAG191510.2167881975574283No Hit
TTCAAGTAATCCAGGATAGGCTT187360.21209042188063165No Hit
CACTAGATTGTGAGCTCCTGGAA175920.1991404089306187No Hit
ACTGGACTTGGAGTCAGAAGGCA170850.1934011986459539No Hit
AACGGAATCCCAAAAGCAGCTG170550.19306160040425774No Hit
TATTGCACTTGTCCCGGCCTGTAG169920.19234844409669585No Hit
TCGTTTCCCGGGCGGCGCACCA168610.19086553177462268No Hit
CCCCCCACTGCTAAATTTGACTGT160040.18116434200350284No Hit
CACTAGATTGTGAGCTCCTGGA157630.1784362361285438No Hit
GGAATACCGGGTGCTGTAGGCTTT156860.1775646006415237No Hit
TAGCAGCACGTAAATATTGGC153300.17353470150672948No Hit
ACTGGACTTGGAGTCAGAAGGCT153060.17326302291337256No Hit
TAGCTTATCAGACTGATGTTGACA152680.17283286514055746No Hit
CTACGCCTGTCTGAGCGTCGCT144410.16347127361113375No Hit
CCCCCCACTGCTAAATTTGACTGTA143430.16236191935492633No Hit
TCGTTTCCCGGCCAATGCACCA142780.1616261231645847No Hit
CAACACCAGTCGATGGGCTGT140540.1590904562932535No Hit
AGCTACATCTGGCTACTGGGTCTC131520.14887986915958945No Hit
CCCATAAAGTAGAAAGCACTA126420.14310669905075502No Hit
AGCAGCATTGTACAGGGCTATGT124610.14105778965918828No Hit
TATTGCACTTGTCCCGGCCTGTAAT124410.14083139083139082No Hit
TATTGCACTTGTCCCGGCCTGTTT121410.1374354084144294No Hit
TATTGCACTTGTCCCGGCCTGTT118000.13357530840048323No Hit
TTCAAGTAATCCAGGATAGGC116310.13166223830559495No Hit
TGAGAACTGAATTCCATAGGCTGT115260.13047364445965845No Hit
CAACGGAATCCCAAAAGCAGCTGA115080.13026988551464075No Hit
AACATTCAACGCTGTCGGTGAGA112240.12705502215991726No Hit
ATCCCGGACGAGCCCCCA109700.12417975704688991No Hit
ATCACATTGCCAGGGATTTCCA108870.12324020191153058No Hit
ATCACATTGCCAGGGATTTCC108780.12313832243902173No Hit
ATGCACCTGGGCAAGGATTCTGA107450.12163277023416884No Hit
CCCCCCACTGCTAAATTTGACTGA107400.12157617052721947No Hit
CGCCTGTCTGAGCGTCGCT106160.12017249779487542No Hit
ACTTGACTTGGAGTCAGAAGGC102460.11598411948062297No Hit
GGAATACCGGGTGCTGTAGGCTT100030.11323337372288421No Hit
TGTAAACATCCCCGACTGGAAGC98320.11129766374521619No Hit
CGCGACCTCAGATCAGACGTGGCG97800.11070902679294287No Hit
ACCACAGGGTAGAACCACGGAC96650.10940723353310766No Hit
TATTGCACTTGTCCCGGCCTGTAAA95840.10849031828052808No Hit
TTCACAGTGGCTAAGTTCCG95090.10764132267628772No Hit
CCCCCACTGCTAAATTTGACTGGCT95020.10756208308655862No Hit
TTCACAGTGGCTAAGTTCCGC94570.10705268572401438No Hit
CAAAGTGCTGTTCGTGCAGGTAG94160.10658856812702966No Hit
AACATTCAACGCTGTCGGTGA93080.10536601445692353No Hit
TGAGGTAGTAGTTTGTACAGTT92300.10448305902851357No Hit
TGTCAGTTTGTCAAATACCCCAA91990.10413214084542756No Hit
CACCCGTAGAACCGACCTTGCG88630.10032864053843074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)