FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694544
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3706376.508633527109457No Hit
AACATTCAACGCTGTCGGTGAGT2610094.583492550061954No Hit
AAGCTGCCAGTTGAAGAACTGT2594404.555939861031892No Hit
CCCCCCACTGCTAAATTTGACTG2127603.7362078508832317No Hit
TTCAAGTAATCCAGGATAGGCT1966073.4525503710218066No Hit
TAGCAGCACGTAAATATTGGCG1826923.2081936674824183No Hit
AACATTCAACGCTGTCGGTGAG1381912.42672635420852No Hit
TAGCTTATCAGACTGATGTTGAC925851.6258545021339725No Hit
TGAGGTAGTAGGTTGTATAGTT854971.5013844831122563No Hit
TAGCTTATCAGACTGATGTTGA803891.4116845879143265No Hit
CGCCTGTCTGAGCGTCGCT719721.2638764403260383No Hit
CCCCCACTGCTAAATTTGACTG686111.2048550331685908No Hit
TAGCTTATCAGACTGATGTTGACT662041.162586503853513No Hit
TATTGCACTTGTCCCGGCCTGT658631.1565983158616389No Hit
TATTGCACTTGTCCCGGCCTGTAA608621.0687774122036813No Hit
TATTGCACTTGTCCCGGCCTGTAT582891.0235938119013568No Hit
TGAGGTAGTAGATTGTATAGTT548350.9629392625643071No Hit
GCCTGTCTGAGCGTCGCT498490.8753817689353177No Hit
CATAAAGTAGAAAGCACTACT438100.7693328912727692No Hit
TGTAAACATCCTTGACTGGAAGCT433480.7612198623805524No Hit
AGCAGCATTGTACAGGGCTATGA409430.7189864544026704No Hit
TTCAAGTAATTCAGGATAGGTT380390.6679902727944503No Hit
AACCGGGCGGAAACACCA327580.5752523819290886No Hit
TATTGCACTTGTCCCGGCCTGTA323700.5684388425131143No Hit
TCGTACGACTCTTAGCGG300970.5285234427901514No Hit
TTAATGCTAATCGTGATAGGGGTT289370.50815306721662No Hit
CCCATAAAGTAGAAAGCACTAC284750.5000400383244031No Hit
ACTGGACTTGGAGTCAGAAGGC257350.451923806366234No Hit
TGTAAACATCCCCGACTGGAAGCT241680.42440623867336874No Hit
CAACGGAATCCCAAAAGCAGCTGT234810.41234205934663076No Hit
TGAGGTAGTAGTTTGTACAGTT224490.39421944935362685No Hit
CTACGCCTGTCTGAGCGTCGCT219140.3848244916537654No Hit
TACGCCTGTCTGAGCGTCGCT217370.3817162533119421No Hit
CCCATAAAGTAGAAAGCACT206740.3630492625924043No Hit
ACGCCTGTCTGAGCGTCGCT197170.3462437027442408No Hit
CATTGCACTTGTCTCGGTCTGA193080.3390613892877112No Hit
TGTAAACATCCTACACTCTCAGCT191120.3356194982425283No Hit
AACATTCAACGCTGTCGGTGAGTT182770.32095633996330525No Hit
TCCTGTCTGAGCGTCGCT178710.3138267085125692No Hit
ATCCCGGACGAGCCCCCA175650.3084531439216204No Hit
TTCTCACTACTGCACTTGACTAG171080.30042791837239297No Hit
ACCGGGTGCTGTAGGCTT148060.26000325926009177No Hit
TGAGGTAGTAGTTTGTGCTGTT145800.2560345481569727No Hit
CAACACCAGTCGATGGGCTGT139650.24523473696928147No Hit
CTCCCACTGCTTCACTTGACTAG139620.24518205496348783No Hit
TGTCAGTTTGTCAAATACCCCAA135730.23835095487891567No Hit
AGCTCGGTCTGAGGCCCCTCAGT122030.21429283889983114No Hit
TGATGTAGTAGGTTGTATAGTT121060.21258945404583757No Hit
TTCACAGTGGCTAAGTTCCG114900.20177208218954845No Hit
GCGTTCGCGCTTTCCCCTG114440.20096429143404634No Hit
AACATTCAACGCTGTCGGTGA111360.1955556055059018No Hit
TTCAAGTAATCCAGGATAGGC111150.1951868314653465No Hit
CACCCGGCTGTGTGCACATGTGC108030.18970790286281042No Hit
TGAGGTAGTAGGTTGTGTGGTT107010.18791671466582752No Hit
TGTAAACATCCTACACTCAGCT105740.18568650975389775No Hit
CGCGACCTCAGATCAGACGTGG105710.18563382774810416No Hit
TTCAAGTAATCCAGGATAGGCTT104630.18373727553953398No Hit
TGTCAGTTTGTCAAATACCCCA103110.18106805391265743No Hit
TAGCAGCACGTAAATATTGGC101890.17892565234371707No Hit
TGAGGTAGTAGGTTGTATAGT100560.1765900834202001No Hit
AACGGAATCCCAAAAGCAGCTG98560.1730779497006257No Hit
ATCCCACCGCTGCCACCA98440.17286722167745125No Hit
CTTCTCACTACTGCACTTGACTAG96140.1688282678999407No Hit
TATTGCACTTGTCCCGGCCTGTT95540.1677746277840684No Hit
TAGCTTATCAGACTGATGTTGACA94050.16515808816298547No Hit
ACCGGGTGCTGTAGGCTTT91860.1613123017400515No Hit
CCCCCACTGCTAAATTTGACTGGCTT89630.1573962726427261No Hit
CAACGGAATCCCAAAAGCAGCT87370.15342756153960704No Hit
AACATTCAACGCTGTCGGTGAGA85580.15028420186058794No Hit
ACCGGGTGCTGTAGGCTTA84420.1482471643032348No Hit
CGCGACCTCAGATCAGACGTGGCG84390.1481944822974412No Hit
TGTAAACATCCCCGACTGGAAGC84340.14810667895445184No Hit
TTATAAAGCAATGAGACTGATT81950.14390967915956046No Hit
TTCACAGTGGCTAAGTTCCGC81820.1436813904677881No Hit
CAAAGAATTCTCCTTTTGGGCT80750.14180239892781582No Hit
TAGCAGCACGTAAATATTGGCGT80080.14062583413175841No Hit
TACCTGGTTGATCCTGCC78610.13804441584787122No Hit
AGCAGCATTGTACAGGGCTATGT77750.13653419834845423No Hit
ACCACAGGGTAGAACCACGGAC77460.13602493895911594No Hit
AGAGGTAGTAGGTTGCATAGTT77340.13581421093594148No Hit
TTCACAGTGGCTAAGTTCTGC77000.13521714820361386No Hit
AGCAGCATTGTACAGGGCTATG76900.13504154151763512No Hit
TGATGTAGTAGATTGTATAGTT76840.1349361775060479No Hit
TGAGAACTGAATTCCATGGGTT75470.13253036590813944No Hit
TGAGAACTGAATTCCATAGGCTGT74780.13131867977488626No Hit
CCCCCCACTGCTAAATTTGACTGGCT74550.13091478439713522No Hit
CCCCCCACTGCTAAATTTGACTGG74340.13054601035657992No Hit
TATTGCACTTGTCCCGGCCTGTAG73920.1298084622754693No Hit
CAAAGTGCTGTTCGTGCAGGT73770.1295450522465012No Hit
GGAATACCGGGTGCTGTAGGCTTT70310.12346906091163752No Hit
ACTGGACTTGGAGTCAGAAGGCA69270.12164275137745884No Hit
TCGTTTCCCGGGCGGCGCACCA66850.11739306957677384No Hit
CAAAGAATTCTCCTTTTGGGCTT66690.1171120988792079No Hit
TCGTTTCCCGGCCAATGCACCA65110.11433751324074412No Hit
GGGCTACGCCTGTCTGAGCGTCGCT64820.11382825385140584No Hit
TGAGGTAGTAGATTGTATAGT63910.1122302330089995No Hit
CCTCGACACAAGGGTTTGT62990.11061465149799526No Hit
GGAATACCGGGTGCTGTAGGCTT61690.10833176458027194No Hit
TTCACCACCTTCTCCACCCAGC61420.10785762652812939No Hit
ACTGGACTTGGAGTCAGAAGG61000.10712007844701876No Hit
CTCCCACTGCTTCACTTGACTAGCCT60490.10622448434852728No Hit
TATTGCACTTGTCCCGGCCTGA60470.10618936301133154No Hit
ACCCCCCACTGCTAAATTTGACTG59270.10408208277958692No Hit
AATGACACGATCACTCCCGTTG59200.10395915809940182No Hit
CAAAGTGCTGTTCGTGCAGGTAG58360.10248406193718057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)