FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10939033
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6910986.317724793407242No Hit
CCCCCCACTGCTAAATTTGACTG5356714.896877082279576No Hit
AACATTCAACGCTGTCGGTGAGT4580034.1868691684173545No Hit
AAGCTGCCAGTTGAAGAACTGT4056393.7081796901060637No Hit
TTCAAGTAATCCAGGATAGGCT3610873.3009042024098476No Hit
TAGCAGCACGTAAATATTGGCG3115822.8483504894811085No Hit
AACATTCAACGCTGTCGGTGAG2738432.503356558116243No Hit
TAGCTTATCAGACTGATGTTGAC2309802.1115211920468653No Hit
TAGCTTATCAGACTGATGTTGACT1826781.6699647948772074No Hit
TAGCTTATCAGACTGATGTTGA1628021.4882668330921023No Hit
TATTGCACTTGTCCCGGCCTGTAA1503661.3745821957023074No Hit
CCCCCACTGCTAAATTTGACTG1465341.339551677008379No Hit
TATTGCACTTGTCCCGGCCTGTAT1351631.235602817909042No Hit
TGAGGTAGTAGGTTGTATAGTT1293041.1820423249477354No Hit
TGTAAACATCCTTGACTGGAAGCT1095881.0018070153001641No Hit
TATTGCACTTGTCCCGGCCTGT917030.8383099310514924No Hit
TGAGGTAGTAGATTGTATAGTT906160.8283730380921238No Hit
GCCTGTCTGAGCGTCGCT866650.7922546718709048No Hit
TTAATGCTAATCGTGATAGGGGTT829910.7586685221627908No Hit
AGCAGCATTGTACAGGGCTATGA827230.7562185798324221No Hit
TATTGCACTTGTCCCGGCCTGTA752320.6877390350682734No Hit
CATAAAGTAGAAAGCACTACT662760.6058670816698332No Hit
TTCAAGTAATTCAGGATAGGTT661310.6045415531701933No Hit
TGTAAACATCCCCGACTGGAAGCT578260.5286207656563428No Hit
CGCCTGTCTGAGCGTCGCT564680.5162065056390268No Hit
CCCATAAAGTAGAAAGCACTAC481690.4403405675803337No Hit
AACCGGGCGGAAACACCA460560.42102441778903127No Hit
ACTGGACTTGGAGTCAGAAGGC451030.4123124959948471No Hit
CAACGGAATCCCAAAAGCAGCTGT431380.3943492994307632No Hit
AACATTCAACGCTGTCGGTGAGTT413940.3784063911316476No Hit
TGTAAACATCCTACACTCTCAGCT394360.3605071855985808No Hit
TGAGGTAGTAGTTTGTACAGTT355060.32458079247041305No Hit
TGAGAACTGAATTCCATAGGCTGT323670.29588538584717683No Hit
TGTCAGTTTGTCAAATACCCCAA312280.2854731309431099No Hit
TTCTCACTACTGCACTTGACTAG309860.2832608695850904No Hit
TTCACAGTGGCTAAGTTCCG302810.2768160586040832No Hit
CATTGCACTTGTCTCGGTCTGA301310.2754448222251455No Hit
TAGCTTATCAGACTGATGTTGACA297070.2715687940606816No Hit
CTACGCCTGTCTGAGCGTCGCT296210.27078261853675734No Hit
CAAAGAATTCTCCTTTTGGGCT290000.26510569992795524No Hit
TCGTACGACTCTTAGCGG274150.2506163021905135No Hit
CCCATAAAGTAGAAAGCACT271020.24775498894646356No Hit
TACGCCTGTCTGAGCGTCGCT258140.2359806392393185No Hit
CTCCCACTGCTTCACTTGACTAG247300.22607117100752872No Hit
TGAGGTAGTAGTTTGTGCTGTT232750.21277017813183305No Hit
CCCCCACTGCTAAATTTGACTGGCTT232090.21216683412510046No Hit
CTTCTCACTACTGCACTTGACTAG227010.20752291358843142No Hit
TGATGTAGTAGGTTGTATAGTT222830.20370173487912505No Hit
TGTCAGTTTGTCAAATACCCCA222570.20346405390677585No Hit
CAACACCAGTCGATGGGCTGT213120.19482526471946834No Hit
TAGCAGCACGTAAATATTGGC210430.19236618081324008No Hit
AGCTCGGTCTGAGGCCCCTCAGT194450.17775794258962377No Hit
GGAATACCGGGTGCTGTAGGCTTT187570.17146853839822954No Hit
TATTGCACTTGTCCCGGCCTGTAG183750.1679764564198682No Hit
TATTGCACTTGTCCCGGCCTGTT183750.1679764564198682No Hit
TTCAAGTAATCCAGGATAGGCTT180090.1646306396552602No Hit
ACGCCTGTCTGAGCGTCGCT179890.16444780813806853No Hit
TTCAAGTAATCCAGGATAGGC176670.16150422071128226No Hit
GGGCTACGCCTGTCTGAGCGTCGCT173310.1584326512224618No Hit
TGTAAACATCCTACACTCAGCT171370.15665918550570238No Hit
CAACGGAATCCCAAAAGCAGCT170740.15608326622654853No Hit
ACTGGACTTGGAGTCAGAAGGCA167610.15322195298249852No Hit
TAGCAGCACGTAAATATTGGCGT167520.15313967879976229No Hit
ACCCCCCACTGCTAAATTTGACTG167340.15297513043428976No Hit
CAAAGAATTCTCCTTTTGGGCTT166020.15176844242082457No Hit
AGCAGCATTGTACAGGGCTATGT165250.15106454107963657No Hit
GCGTTCGCGCTTTCCCCTG164800.15065317016595525No Hit
CCCCCCACTGCTAAATTTGACTGGCT161580.147709582739169No Hit
TGTAAACATCCCCGACTGGAAGC160390.1466217352118784No Hit
CACCCGGCTGTGTGCACATGTGC158890.14525049883294072No Hit
AACATTCAACGCTGTCGGTGAGA157270.143769563543688No Hit
CCCCCCACTGCTAAATTTGACTGG156920.14344960838860255No Hit
TGATGTAGTAGATTGTATAGTT156770.14331248475070876No Hit
AGCTACATTGTCTGCTGGGTTT155550.14219721249583944No Hit
AACGGAATCCCAAAAGCAGCTG151640.13862285633474183No Hit
TGAGGTAGTAGGTTGTGTGGTT150340.13743445147299582No Hit
CGCGACCTCAGATCAGACGTGGCG147680.1350027922943463No Hit
AACATTCAACGCTGTCGGTGA145800.1332841760327444No Hit
TGAGAACTGAATTCCATGGGTT145540.13304649506039518No Hit
TTAATGCTAATCGTGATAGGGGTTT144260.13187637335036834No Hit
GGAATACCGGGTGCTGTAGGCTT142660.1304137212128348No Hit
GTCAGGATGGCCGAGCGGTCTAAGG137640.1258246501313233No Hit
TTATAAAGCAATGAGACTGATT136640.12491049254536483No Hit
AGCAGCATTGTACAGGGCTATG134730.12316445155618418No Hit
TGAGGTAGTAGGTTGTATAGT131640.1203397046155725No Hit
CTCCCACTGCTTCACTTGACTAGCCT130210.1190324592676519No Hit
ATCCCGGACGAGCCCCCA128010.11702131257854329No Hit
ATCACATTGCCAGGGATTTCCA125350.11458965339989374No Hit
CAAAGTGCTGTTCGTGCAGGTAG124210.11354751375190111No Hit
AGCTACATCTGGCTACTGGGTCTC122380.11187460536959712No Hit
AGCTACATCTGGCTACTGGGTCT120850.11047594426308066No Hit
TCGTTTCCCGGCCAATGCACCA119720.1094429461909476No Hit
ACTGGACTTGGAGTCAGAAGGCT119170.10894015951867045No Hit
ATCACATTGCCAGGGATTTCC117620.10752321526043482No Hit
TTCACAGTGGCTAAGTTCTGC116390.10639880142970591No Hit
TCGTTTCCCGGGCGGCGCACCA116050.10608798785048001No Hit
ACCGGGTGCTGTAGGCTT115960.10600571366774375No Hit
CGCGACCTCAGATCAGACGTGG115470.10555777645062411No Hit
TTCACAGTGGCTAAGTTCTG114940.10507327293006613No Hit
TAGCAGCACATCATGGTTTACA114350.10453391995435063No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT113540.10379345230972427No Hit
CAAAGTGCTGTTCGTGCAGGT112890.10319924987885126No Hit
ACCACAGGGTAGAACCACGGAC112690.10301641836165958No Hit
ACTGGACTTGGAGTCAGAAGG112580.10291586102720415No Hit
TTAATGCTAATCGTGATAGGGGT112540.10287929472376581No Hit
TATTGCACTTGTCCCGGCCTGTTT110190.1007310243967634No Hit
TTCACAGTGGCTAAGTTCCGC109420.10002712305557537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)