FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5085987
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4558878.963589564818No Hit
CCCCCCACTGCTAAATTTGACTG2127514.183081867885231No Hit
AACATTCAACGCTGTCGGTGAGT1621843.188840238875955No Hit
TTCAAGTAATCCAGGATAGGCT1606473.1586199492841804No Hit
CGCCTGTCTGAGCGTCGCT883061.7362608280359348No Hit
AACATTCAACGCTGTCGGTGAG824171.6204720932239898No Hit
TAGCAGCACGTAAATATTGGCG778531.5307353321980572No Hit
CCCCCACTGCTAAATTTGACTG777421.5285528649601345No Hit
AAGCTGCCAGTTGAAGAACTGT760441.495167014780022No Hit
GCCTGTCTGAGCGTCGCT705121.386397566490044No Hit
CATAAAGTAGAAAGCACTACT595201.1702743243346867No Hit
TAGCTTATCAGACTGATGTTGA404860.79603034769849No Hit
CCCATAAAGTAGAAAGCACT355870.6997068612247731No Hit
CCCATAAAGTAGAAAGCACTAC320630.6304184418874842No Hit
TCGTACGACTCTTAGCGG315990.6212953355956278No Hit
TAGCTTATCAGACTGATGTTGAC292010.5741461785096973No Hit
AGCAGCATTGTACAGGGCTATGA291190.5725339054150158No Hit
CAACACCAGTCGATGGGCTGT287030.5643545687395582No Hit
ACTGGACTTGGAGTCAGAAGGC286960.5642169356705001No Hit
CAACGGAATCCCAAAAGCAGCTGT277260.5451449246724382No Hit
TACGCCTGTCTGAGCGTCGCT271280.5333871282014682No Hit
TCCTGTCTGAGCGTCGCT263720.5185227567431848No Hit
CTACGCCTGTCTGAGCGTCGCT247720.4870637695298868No Hit
CATTGCACTTGTCTCGGTCTGA231120.45442507029609003No Hit
ACGCCTGTCTGAGCGTCGCT224760.44192012287880406No Hit
TGAGGTAGTAGGTTGTATAGTT213900.42056733530777807No Hit
ATCCCGGACGAGCCCCCA196000.38537259336290086No Hit
TTCAAGTAATTCAGGATAGGTT188480.3705868693726508No Hit
TTCACAGTGGCTAAGTTCCG178440.3508463548963063No Hit
ACCGGGTGCTGTAGGCTT158870.3123680811610411No Hit
AACCGGGCGGAAACACCA158610.311856872618825No Hit
TATTGCACTTGTCCCGGCCTGT156640.30798348481818766No Hit
TGTAAACATCCTACACTCTCAGCT153020.300865888961179No Hit
CTCCCACTGCTTCACTTGACTAG147070.2891670780912338No Hit
TAGCTTATCAGACTGATGTTGACT146240.287535143129544No Hit
CAACGGAATCCCAAAAGCAGCT146180.2874171719274941No Hit
AACATTCAACGCTGTCGGTGAGTT144450.28401566893505625No Hit
TATTGCACTTGTCCCGGCCTGTAA140980.27719300108317224No Hit
CCCCCACTGCTAAATTTGACTGGCTT132300.26012650051995806No Hit
GCGTTCGCGCTTTCCCCTG128570.25279262412585796No Hit
TGAGGTAGTAGATTGTATAGTT127940.25155392650433434No Hit
CGCGACCTCAGATCAGACGT119320.23460539714317005No Hit
AACGGAATCCCAAAAGCAGCTG113830.22381103215560716No Hit
TACCTGGTTGATCCTGCC109490.21527778187400004No Hit
ATCCCACCGCTGCCACCA106280.20896632256433215No Hit
CACCCGGCTGTGTGCACATGTGC104980.2064102798532517No Hit
CCCCCCACTGCTAAATTTGACTGGCT103430.20336269046696345No Hit
TATTGCACTTGTCCCGGCCTGTAT100600.19779838210361136No Hit
ACCGGGTGCTGTAGGCTTT100070.19675630315217088No Hit
TAATGTGAATTGCAGGACA94040.18490019734615915No Hit
CCCATAAAGTAGAAAGCACTA90620.17817583882931673No Hit
TGTAAACATCCTACACTCAGCT90420.17778260148915048No Hit
ACCACAGGGTAGAACCACGGAC88560.17412549422560458No Hit
ACCGGGTGCTGTAGGCTTA87150.1713531709774327No Hit
CAAAGAATTCTCCTTTTGGGCT86320.16972123601574288No Hit
ACTGGACTTGGAGTCAGAAGG85730.1685611858622525No Hit
CGCGACCTCAGATCAGACGTGG84610.16635905675732165No Hit
TTCAAGTAATCCAGGATAGGCTT84320.1657888626140806No Hit
CGCGACCTCAGATCAGACGTGGC80170.15762918780563143No Hit
CGCGACCTCAGATCAGACGTGGCG77640.15265473545252867No Hit
CTCGACACAAGGGTTTGT77080.15155367090006325No Hit
TATTGCACTTGTCCCGGCCTGTA75970.1493712036621407No Hit
ACTGGACTTGGAGTCAGAAGGCT74520.14652023294593558No Hit
TTCTCACTACTGCACTTGACTAG74020.14553713959552No Hit
CCTCGACACAAGGGTTTGT72550.14264684514529824No Hit
ACTGGACTTGGAGTCAGAAGGCA72350.14225360780513202No Hit
CTTCTCACTACTGCACTTGACTAG71900.141368823789758No Hit
TTCAAGTAATCCAGGATAGGC71530.14064133471045048No Hit
CCCCCCACTGCTAAATTTGACTGG70640.1388914285467108No Hit
AGAATTACTCTGAGACCT69900.13743645038809577No Hit
ATACCGGGTGCTGTAGGCTT67450.1326192929710595No Hit
TTCACAGTGGCTAAGTTCCGC65300.12839199156427258No Hit
AGCTCGGTCTGAGGCCCCTCAGT65280.12835266783025595No Hit
AACATTCAACGCTGTCGGTGA63490.12483319363576825No Hit
CAAAGAATTCTCCTTTTGGGCTT63230.12432198509355215No Hit
TGTAAACATCCTTGACTGGAAGCT63190.1242433376255189No Hit
CAAAGTGCTGTTCGTGCAGGT62370.12263106453083737No Hit
ATACCGGGTGCTGTAGGCTTA62370.12263106453083737No Hit
ATAGGCGATAGAAATTGAAACCT61740.12139236690931376No Hit
ATACCGGGTGCTGTAGGCTTT61600.12111710077119742No Hit
AGCAGCATTGTACAGGGCTATGT61300.12052724476094807No Hit
TCGTTCGCGCTTTCCCCTG59010.1160246772160448No Hit
AATGACACGATCACTCCCGTTG58780.11557245427485363No Hit
TGTCAGTTTGTCAAATACCCCAA57420.11289844036172329No Hit
AGCAGCATTGTACAGGGCTATG55990.11008679337953478No Hit
CTTGACTAGCTTGCTGTTT53510.1052106503614736No Hit
CCAAGTCCTTCTGATCGAGGCCC53070.10434552821310789No Hit
ATCACATTGCCAGGGATTTCC52590.10340175859670897No Hit
TCGTTTCCCGGCCAATGCACCA51770.10178948550202743No Hit
CACTAGATTGTGAGCTCCTGGA51250.10076706841759525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)