FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6176026
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3985656.453421666294798No Hit
CCCCCCACTGCTAAATTTGACTG3746576.0663118970030245No Hit
AAGCTGCCAGTTGAAGAACTGT2985974.834775630802072No Hit
TAGCAGCACGTAAATATTGGCG2298973.7224098473678704No Hit
TTCAAGTAATCCAGGATAGGCT2127793.445241325085095No Hit
GCCTGTCTGAGCGTCGCT2014813.262308157381462No Hit
AACATTCAACGCTGTCGGTGAGT1784712.8897384823185654No Hit
TAGCTTATCAGACTGATGTTGAC1581452.5606271735255No Hit
TAGCTTATCAGACTGATGTTGACT1291412.0910047982310958No Hit
TGTAAACATCCTTGACTGGAAGCT967811.567043273457722No Hit
TATTGCACTTGTCCCGGCCTGTAA936841.5168977591739412No Hit
CCCCCACTGCTAAATTTGACTG925061.497824005274589No Hit
AACATTCAACGCTGTCGGTGAG924691.4972249145324195No Hit
TATTGCACTTGTCCCGGCCTGTAT912411.477341578549054No Hit
TAGCTTATCAGACTGATGTTGA658391.0660414965869638No Hit
TGAGGTAGTAGGTTGTATAGTT570520.9237655411424758No Hit
TATTGCACTTGTCCCGGCCTGT559100.9052746863436132No Hit
AGCAGCATTGTACAGGGCTATGA550450.8912689162901839No Hit
AACCGGGCGGAAACACCA506920.8207867000559907No Hit
CATAAAGTAGAAAGCACTACT450720.7297896738129017No Hit
TGTAAACATCCCCGACTGGAAGCT447020.7237987663912037No Hit
TCCTGTCTGAGCGTCGCT446100.7223091353566193No Hit
TTCAAGTAATTCAGGATAGGTT441890.7154924542092277No Hit
TGAGGTAGTAGATTGTATAGTT420120.680243250271291No Hit
TGTAAACATCCTACACTCTCAGCT412710.6682452437862146No Hit
TATTGCACTTGTCCCGGCCTGTA383100.6203017927709501No Hit
ACTGGACTTGGAGTCAGAAGGC363690.5888738162695558No Hit
CAACGGAATCCCAAAAGCAGCTGT310120.5021351917883766No Hit
CGCCTGTCTGAGCGTCGCT269040.4356199277658481No Hit
CCCATAAAGTAGAAAGCACTAC256650.4155584837239999No Hit
CTTCTCACTACTGCACTTGACTAG223650.3621260661791255No Hit
TCGTACGACTCTTAGCGG212830.3446067098810789No Hit
TTCTCACTACTGCACTTGACTAG204330.33084381445285366No Hit
CAAAGAATTCTCCTTTTGGGCT200930.32533865628156355No Hit
CATTGCACTTGTCTCGGTCTGA199600.3231851679380884No Hit
GGAATACCGGGTGCTGTAGGCTTT188070.3045162050807429No Hit
TGAGGTAGTAGTTTGTACAGTT186730.30234652509558735No Hit
TAGCTTATCAGACTGATGTTGACA186320.3016826677866965No Hit
CCCATAAAGTAGAAAGCACT179650.29088284278595977No Hit
CAAAGAATTCTCCTTTTGGGCTT176710.2861225001319619No Hit
AACATTCAACGCTGTCGGTGAGTT170620.27626179034868054No Hit
TGTCAGTTTGTCAAATACCCCAA167950.2719386220200498No Hit
TTCAAGTAATCCAGGATAGGCTT151030.24454236429704151No Hit
TGTAAACATCCTACACTCAGCT148440.2403487291018529No Hit
TGTCAGTTTGTCAAATACCCCA145890.2362198604733853No Hit
CTCCCACTGCTTCACTTGACTAG142940.23144332617770716No Hit
AGCTCGGTCTGAGGCCCCTCAGT138180.223736104737901No Hit
TGAGGTAGTAGTTTGTGCTGTT131440.21282293824540247No Hit
TGTAAACATCCCCGACTGGAAGC123650.20020964937647606No Hit
CTACGCCTGTCTGAGCGTCGCT123220.1995134087842247No Hit
GGAATACCGGGTGCTGTAGGCTT117660.19051085600999737No Hit
CCCCCCACTGCTAAATTTGACTGGCT117300.18992795690950784No Hit
TACGCCTGTCTGAGCGTCGCT115010.18622007096472715No Hit
TAGCAGCACGTAAATATTGGC114480.1853619139556731No Hit
CCCCCACTGCTAAATTTGACTGGCTT112570.1822693103947425No Hit
AGCAGCATTGTACAGGGCTATGT110860.1795005396674172No Hit
CAACGGAATCCCAAAAGCAGCT108950.17640793610648658No Hit
TAGCAGCACGTAAATATTGGCGT108440.1755821623807931No Hit
ATCACATTGCCAGGGATTTCCA108410.1755335874557523No Hit
TTCACAGTGGCTAAGTTCCG107400.17389823164604554No Hit
TATTGCACTTGTCCCGGCCTGTT107300.17373631522924288No Hit
CCCCCCACTGCTAAATTTGACTGG106830.17297530807027045No Hit
TTATAAAGCAATGAGACTGATT105530.17087039465183598No Hit
CAACACCAGTCGATGGGCTGT100910.16338985619555357No Hit
TATTGCACTTGTCCCGGCCTGTAG100670.1630012567952272No Hit
CACTAGATTGTGAGCTCCTGGAA100130.16212690814449293No Hit
TGTCAGTTTGTCAAATACCCC99160.16055631890150723No Hit
CACTAGATTGTGAGCTCCTGGA98020.158710471749957No Hit
ACTGGACTTGGAGTCAGAAGGCA90100.14588669153918718No Hit
TTCAAGTAATCCAGGATAGGC89140.14433229393788172No Hit
TATTGCACTTGTCCCGGCCTGTTT86920.14073774948486292No Hit
AACGGAATCCCAAAAGCAGCTG85320.13814708681602053No Hit
TTCACAGTGGCTAAGTTCCGC84890.13745084622376916No Hit
TATTGCACTTGTCCCGGCCTGTAAT81950.13269050356977125No Hit
GCGTTCGCGCTTTCCCCTG80230.12990554120076567No Hit
ACCACAGGGTAGAACCACGGAC79250.1283187603160997No Hit
ACGCCTGTCTGAGCGTCGCT77440.12538807317197176No Hit
TGTAAACATCCTTGACTGGAAGC77420.1253556898886112No Hit
TTCACAGTGGCTAAGTTCTGC76760.12428704153771372No Hit
TCGTTTCCCGGCCAATGCACCA76290.12352603437874128No Hit
CAAAGTGCTGTTCGTGCAGGTAG74900.12127539618518444No Hit
CAGTGCAATAGTATTGTCAAAGC74590.12077345529309623No Hit
TCGTTTCCCGGGCGGCGCACCA73200.11852281709953941No Hit
ATCACATTGCCAGGGATTTCC72980.11816660098257357No Hit
ACTGGACTTGGAGTCAGAAGGCT72810.11789134307400907No Hit
ACTGGACTTGGAGTCAGAAGG70060.1134386416119362No Hit
TGATGTAGTAGGTTGTATAGTT69980.1133091084784941No Hit
ATGCACCTGGGCAAGGATTCTGA69890.11316338370337173No Hit
CCAAGTCCTTCTGATCGAGGCCC67890.10992505536731874No Hit
AGCAGCATTGTACAGGGCTATG67720.10964979745875422No Hit
TAGCAGCACATCATGGTTTACA66750.10807920821576852No Hit
CACCCGGCTGTGTGCACATGTGC63640.10304360765320614No Hit
ACCCCCCACTGCTAAATTTGACTG63420.1026873915362403No Hit
TATTGCACTTGTCCCGGCCTGTAAA61900.10022626200084002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)