FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8971471
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8060398.984468656254922No Hit
CCCCCCACTGCTAAATTTGACTG4482914.996850572219428No Hit
AAGCTGCCAGTTGAAGAACTGT3947964.4005715450676925No Hit
TAGCAGCACGTAAATATTGGCG3158933.5210836662125975No Hit
TTCAAGTAATCCAGGATAGGCT3103123.458875361688178No Hit
AACATTCAACGCTGTCGGTGAGT3008963.3539204440386645No Hit
GCCTGTCTGAGCGTCGCT2116882.359568458728786No Hit
AACATTCAACGCTGTCGGTGAG1940362.1628114274682493No Hit
CCCCCACTGCTAAATTTGACTG1812962.020805729628954No Hit
TAGCTTATCAGACTGATGTTGAC1556671.7351335137794015No Hit
TAGCTTATCAGACTGATGTTGACT1228131.368928239304346No Hit
TGAGGTAGTAGGTTGTATAGTT1161841.2950384613626906No Hit
CATAAAGTAGAAAGCACTACT1032391.15074774248281No Hit
TAGCTTATCAGACTGATGTTGA940141.047921795656476No Hit
TATTGCACTTGTCCCGGCCTGTAA913421.0181384970201652No Hit
TGTAAACATCCTTGACTGGAAGCT865950.9652263268754924No Hit
TCCTGTCTGAGCGTCGCT831150.9264367014060458No Hit
TATTGCACTTGTCCCGGCCTGTAT784050.8739369496930882No Hit
TATTGCACTTGTCCCGGCCTGT767050.8549879947223816No Hit
CGCCTGTCTGAGCGTCGCT682060.7602543663129491No Hit
TGAGGTAGTAGATTGTATAGTT635980.7088915518982338No Hit
TCGTACGACTCTTAGCGG628100.7001081539471063No Hit
AACCGGGCGGAAACACCA576990.643138678149882No Hit
AGCAGCATTGTACAGGGCTATGA554140.6176690533804322No Hit
CAACGGAATCCCAAAAGCAGCTGT536010.5974605502263787No Hit
TTCAAGTAATTCAGGATAGGTT479170.5341041619596162No Hit
CCCATAAAGTAGAAAGCACTAC479170.5341041619596162No Hit
ACTGGACTTGGAGTCAGAAGGC471990.5261010150955178No Hit
CCCATAAAGTAGAAAGCACT463540.5166822698306666No Hit
TATTGCACTTGTCCCGGCCTGTA450230.5018463527330134No Hit
TGTAAACATCCCCGACTGGAAGCT424760.47345635960925475No Hit
TGTCAGTTTGTCAAATACCCCAA404590.4509739818587164No Hit
CAACGGAATCCCAAAAGCAGCT385920.43016357072324035No Hit
CATTGCACTTGTCTCGGTCTGA331640.36966067214618425No Hit
TTAATGCTAATCGTGATAGGGGTT312540.34837096391439043No Hit
TGAGGTAGTAGTTTGTACAGTT293250.3268694732446886No Hit
TGTAAACATCCTACACTCTCAGCT286260.31907810881849813No Hit
CAACACCAGTCGATGGGCTGT277180.3089571375753207No Hit
CACCCGTAGAACCGACCTTGCG250910.2796754289235288No Hit
AACGGAATCCCAAAAGCAGCTG231660.2582185240302287No Hit
TGTCAGTTTGTCAAATACCCCA216890.2417552260939148No Hit
AACATTCAACGCTGTCGGTGAGTT208390.2322807486085615No Hit
TTCTCACTACTGCACTTGACTAG205730.22931579447785094No Hit
TAGCAGCACGTAAATATTGGC199350.22220436314178577No Hit
CTACGCCTGTCTGAGCGTCGCT198690.22146869783115836No Hit
TACGCCTGTCTGAGCGTCGCT195400.2178015177221216No Hit
TGAGGTAGTAGTTTGTGCTGTT190640.21249581033032378No Hit
TCGTTTCCCGGCCAATGCACCA165530.1845070891941801No Hit
TAGCTTATCAGACTGATGTTGACA158290.17643706366547915No Hit
AGCTCGGTCTGAGGCCCCTCAGT153880.17152148181719587No Hit
TTATAAAGCAATGAGACTGATT152520.17000556541953935No Hit
CAAAGAATTCTCCTTTTGGGCT149360.166483289083808No Hit
ACGCCTGTCTGAGCGTCGCT148500.16552469489117227No Hit
ATCCCACCGCTGCCACCA146700.16351833495309745No Hit
TTCACAGTGGCTAAGTTCTGC146290.1630613307449804No Hit
ATCCCGGACGAGCCCCCA145450.16212502944054547No Hit
TTCAAGTAATCCAGGATAGGCTT143520.15997376572916527No Hit
TTCAAGTAATCCAGGATAGGC140980.15714256892765968No Hit
TGAGGTAGTAGGTTGTGTGGTT139500.15549289520079818No Hit
TTCACAGTGGCTAAGTTCCGC138880.1548018156665724No Hit
CTTCTCACTACTGCACTTGACTAG135780.15134641799544357No Hit
CAACGGAATCCCAAAAGCAGCTGA134450.14986394093008829No Hit
TTCACAGTGGCTAAGTTCCG128090.14277480248222393No Hit
ACCGGGTGCTGTAGGCTT126030.1404786349975383No Hit
TGTAAACATCCCCGACTGGAAGC123970.13818246751285268No Hit
ACTGGACTTGGAGTCAGAAGGCT123580.13774775619293647No Hit
CACCCGGCTGTGTGCACATGTGC123400.13754712019912899No Hit
CAAAGAATTCTCCTTTTGGGCTT122190.1361984004629787No Hit
TGTAAACATCCTACACTCAGCT122100.13609808246607497No Hit
ACCACAGGGTAGAACCACGGAC119830.1335678396552806No Hit
TATTGCACTTGTCCCGGCCTGTT119040.13268727057134777No Hit
CCCCCACTGCTAAATTTGACTGGCTT118020.1315503332731054No Hit
TGAGAACTGAATTCCATAGGCTGT116450.12980034154934011No Hit
TAGCAGCACGTAAATATTGGCGT113550.12656787276021958No Hit
ACTGGACTTGGAGTCAGAAGGCA112660.1255758392352826No Hit
ACTGGACTTGGAGTCAGAAGG112180.12504080991846264No Hit
CACTAGATTGTGAGCTCCTGGA110640.12332425752699865No Hit
TGAGAACTGAATTCCATGGGTT110470.12313476797729157No Hit
ATCACATTGCCAGGGATTTCC107190.11947873431235524No Hit
AGCAGCATTGTACAGGGCTATGT105970.11811886813210452No Hit
CCCCCCACTGCTAAATTTGACTGG103550.11542142865980395No Hit
TGAGGTAGTAGGTTGTATAGT100700.11224469209118548No Hit
AACATTCAACGCTGTCGGTGA99580.11099629035193895No Hit
CTCCCACTGCTTCACTTGACTAG98330.10960298483938698No Hit
CCCATAAAGTAGAAAGCACTA98330.10960298483938698No Hit
AGAGGTAGTAGGTTGCATAGTT97880.10910139485486828No Hit
TATTGCACTTGTCCCGGCCTGTAG97630.10882273375235789No Hit
TGTCAGTTTGTCAAATACCCC94080.10486574609671034No Hit
CACTAGATTGTGAGCTCCTGGAA92900.10355046569286129No Hit
ACCGGGTGCTGTAGGCTTT91900.10243582128281972No Hit
TTCACAGTGGCTAAGTTCTG90730.1011316873230711No Hit
CAAAGTGCTGTTCGTGCAGGT90530.10090875844106278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)