FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5337747
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5144029.63706222868937No Hit
CCCCCCACTGCTAAATTTGACTG4701418.807854699745041No Hit
TTCAAGTAATCCAGGATAGGCT2112423.9575124111352595No Hit
TAGCAGCACGTAAATATTGGCG1745903.270855662510794No Hit
AAGCTGCCAGTTGAAGAACTGT1605683.0081605591272873No Hit
CCCCCACTGCTAAATTTGACTG1343622.5172043560700796No Hit
AACATTCAACGCTGTCGGTGAGT1280102.398202837264486No Hit
TAGCTTATCAGACTGATGTTGAC1133712.1239485498282327No Hit
AACATTCAACGCTGTCGGTGAG1106092.072203871783357No Hit
TAGCTTATCAGACTGATGTTGACT986941.8489823515427015No Hit
TATTGCACTTGTCCCGGCCTGTAA874911.6390997924779875No Hit
TGTAAACATCCTTGACTGGAAGCT760321.424421202428665No Hit
TATTGCACTTGTCCCGGCCTGTAT739471.3853597781985547No Hit
TGAGGTAGTAGGTTGTATAGTT609941.1426918510749946No Hit
TAGCTTATCAGACTGATGTTGA570961.0696647855359198No Hit
TTCAAGTAATTCAGGATAGGTT547431.0255825163687975No Hit
TTAATGCTAATCGTGATAGGGGTT439770.8238869320707782No Hit
TGAGGTAGTAGATTGTATAGTT425800.7977148411118025No Hit
GGAATACCGGGTGCTGTAGGCTTT416090.7795236454631514No Hit
AGCAGCATTGTACAGGGCTATGA369610.692445707898857No Hit
CAACGGAATCCCAAAAGCAGCT369500.6922396284424871No Hit
TATTGCACTTGTCCCGGCCTGT354250.6636695219912072No Hit
CAACGGAATCCCAAAAGCAGCTGT347280.6506115782557698No Hit
CATAAAGTAGAAAGCACTACT341020.6388837837387198No Hit
TATTGCACTTGTCCCGGCCTGTA330040.6183133070937982No Hit
TGTAAACATCCCCGACTGGAAGCT327290.613161320684551No Hit
CCCATAAAGTAGAAAGCACTAC293700.5502321485076006No Hit
CAAAGAATTCTCCTTTTGGGCT264440.4954150131132105No Hit
TGTAAACATCCTACACTCTCAGCT232280.4351648738690687No Hit
CCCCCACTGCTAAATTTGACTGGCTT222880.41755444759746013No Hit
GGAATACCGGGTGCTGTAGGCTT205960.38585568030856465No Hit
ACTGGACTTGGAGTCAGAAGGC203630.38149054273272975No Hit
GCCTGTCTGAGCGTCGCT188210.3526019498488782No Hit
TAGCTTATCAGACTGATGTTGACA187930.35207738395993665No Hit
TGAGAACTGAATTCCATAGGCTGT182070.3410989692842317No Hit
TTCTCACTACTGCACTTGACTAG180340.3378579014704144No Hit
TGAGAACTGAATTCCATGGGTT178880.335122665049505No Hit
CATTGCACTTGTCTCGGTCTGA172010.3222520662744038No Hit
TGAGGTAGTAGTTTGTACAGTT167690.3141587639878773No Hit
TCGTTTCCCGGCCAATGCACCA158070.2961361788035289No Hit
AACCGGGCGGAAACACCA154120.28873605287024656No Hit
CCCATAAAGTAGAAAGCACT153210.28703121373118656No Hit
CAAAGAATTCTCCTTTTGGGCTT129040.24174993681791213No Hit
CTTCTCACTACTGCACTTGACTAG124810.23382524499568824No Hit
TAGCAGCACGTAAATATTGGC122740.22994720431672766No Hit
CCCCCCACTGCTAAATTTGACTGGCT116820.21885638266482094No Hit
TGTCAGTTTGTCAAATACCCCA115160.215746456323239No Hit
TGAGGTAGTAGTTTGTGCTGTT110010.20609819086592152No Hit
CAACGGAATCCCAAAAGCAGCTGA107880.20210774321075914No Hit
CACCCGTAGAACCGACCTTGCG106140.19884794090090818No Hit
AACGGAATCCCAAAAGCAGCTG105570.1977800746269915No Hit
CACTAGATTGTGAGCTCCTGGAA102880.19274049519394607No Hit
AGCTCGGTCTGAGGCCCCTCAGT99910.18717634987195908No Hit
CTCCCACTGCTTCACTTGACTAG99430.18627709406234505No Hit
AACATTCAACGCTGTCGGTGAGTT98600.18472213089155406No Hit
CCCCCCACTGCTAAATTTGACTGG98100.18378540608987276No Hit
TGAATACCGGGTGCTGTAGGCTTT97600.18284868128819143No Hit
ACCCCCCACTGCTAAATTTGACTG96500.18078788672449256No Hit
TTCAAGTAATCCAGGATAGGCTT96430.18065674525225717No Hit
TGTCAGTTTGTCAAATACCCCAA95910.17968255145850862No Hit
TATTGCACTTGTCCCGGCCTGTAG94320.17670376658916206No Hit
TTCACAGTGGCTAAGTTCCG93120.1744556270651269No Hit
TATTGCACTTGTCCCGGCCTGTAAA91140.17074619685046893No Hit
ATCACATTGCCAGGGATTTCC88410.165631679433289No Hit
ATGCACCTGGGCAAGGATTCTGA88210.16525698951261647No Hit
CACTAGATTGTGAGCTCCTGGA87320.16358961936562372No Hit
TTAATGCTAATCGTGATAGGGGTTT86370.16180984224242925No Hit
GGGAATACCGGGTGCTGTAGGCTTT85430.16004879961526838No Hit
TTCAAGTAATCCAGGATAGGC85250.1597115786866631No Hit
TATTGCACTTGTCCCGGCCTGTAAT84430.15817535001190577No Hit
TGTAAACATCCCCGACTGGAAGC83560.1565454488569803No Hit
TATTGCACTTGTCCCGGCCTGTT81390.1524800632176834No Hit
TTCACAGTGGCTAAGTTCTGC76580.14346877062550922No Hit
ATCACATTGCCAGGGATTTCCA76220.1427943287682987No Hit
TCGTACGACTCTTAGCGG74420.13942211948224598No Hit
CCCATAAAGTAGAAAGCACTA72610.13603117570015963No Hit
CAACACCAGTCGATGGGCTGT72400.13563775128345348No Hit
TGATGTAGTAGGTTGTATAGTT71840.1345886195055704No Hit
CACCCGGCTGTGTGCACATGTGC71020.13305239083081308No Hit
GGAATACCGGGTGCTGTAGGCTTA70230.1315723656441566No Hit
GAATACCGGGTGCTGTAGGCTTT67540.12653278621111116No Hit
AGCAGCATTGTACAGGGCTATGT65350.12242993157974703No Hit
TGTAAACATCCTTGACTGGAAGC64320.12050027848828353No Hit
TATTGCACTTGTCCCGGCCTGTTT64150.1201817920557119No Hit
CTACGCCTGTCTGAGCGTCGCT63420.11881417384525719No Hit
TTATAAAGCAATGAGACTGATT61520.11525461959886821No Hit
CTCCCACTGCTTCACTTGACTAGCCT60720.11375585991617812No Hit
TCGTTTCCCGGGCGGCGCACCA60680.11368092193204361No Hit
GGGAATACCGGGTGCTGTAGGCTT60310.11298774557879944No Hit
CAAAGTGCTGTTCGTGCAGGTAG60010.11242571069779066No Hit
TGTAAACATCCTACACTCAGCT59170.11085201303096606No Hit
TAGCAGCACATCATGGTTTACA58990.1105147921023608No Hit
TTCAAGTAATTCAGGATAGGTTA58790.11014010218168827No Hit
CAAAGAATTCTCCTTTTGGGC57740.10817298009815751No Hit
TTCACAGTGGCTAAGTTCTG57520.10776082118541776No Hit
CCCCCCACTGCTAAATTTGACTGTA57490.10770461769731687No Hit
AGCTACATTGTCTGCTGGGTTT55080.10318960415321296No Hit
TTAATGCTAATCGTGATAGGGGT55010.10305846268097757No Hit
CAGTGCAATAGTATTGTCAAAGC55000.10303972818494396No Hit
TTCAAGTAATCCAGGATAGGCTA54720.10251516229600242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)