FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9146780
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG100489110.986281511089148No Hit
TTCAAGTAATCCAGGATAGGCT4369874.777495468350611No Hit
TAGCAGCACGTAAATATTGGCG4151644.538908774453961No Hit
CCCCCCACTGCTAAATTTGACTG3918854.284403910447174No Hit
AAGCTGCCAGTTGAAGAACTGT3500373.8268877134904304No Hit
AACATTCAACGCTGTCGGTGAGT2157532.358786370722812No Hit
AACATTCAACGCTGTCGGTGAG1990892.176602039187561No Hit
CATAAAGTAGAAAGCACTACT1714201.874102143049248No Hit
TAGCTTATCAGACTGATGTTGAC1529731.6724246128145641No Hit
TATTGCACTTGTCCCGGCCTGTAA1430001.56339170724561No Hit
TAGCTTATCAGACTGATGTTGACT1399081.5295874613798517No Hit
CCCCCACTGCTAAATTTGACTG1347161.4728243163167805No Hit
TATTGCACTTGTCCCGGCCTGTAT1218951.3326547703126128No Hit
TGTAAACATCCTTGACTGGAAGCT1201691.3137847417342496No Hit
TAGCTTATCAGACTGATGTTGA1092251.1941360784888233No Hit
TGAGGTAGTAGGTTGTATAGTT1013271.107788751888643No Hit
TATTGCACTTGTCCCGGCCTGT814930.8909474153745909No Hit
TTCAAGTAATTCAGGATAGGTT789140.8627517005984621No Hit
TGAGGTAGTAGATTGTATAGTT662550.72435326967523No Hit
GCCTGTCTGAGCGTCGCT656840.7181106356553891No Hit
CAACGGAATCCCAAAAGCAGCTGT620540.6784245384714621No Hit
CCCATAAAGTAGAAAGCACTAC613390.670607579935234No Hit
TATTGCACTTGTCCCGGCCTGTA604290.6606587236163983No Hit
AGCAGCATTGTACAGGGCTATGA587440.64223694021284No Hit
TGTAAACATCCCCGACTGGAAGCT479300.5240095421558187No Hit
TCGTACGACTCTTAGCGG425260.4649286415547329No Hit
TTCTCACTACTGCACTTGACTAG400080.43739982813624023No Hit
CCCATAAAGTAGAAAGCACT396030.4329720404339013No Hit
CATTGCACTTGTCTCGGTCTGA372860.40764072165286586No Hit
CCCCCACTGCTAAATTTGACTGGCTT369910.4044155429561004No Hit
TGTAAACATCCTACACTCTCAGCT367810.4021196530363691No Hit
ACTGGACTTGGAGTCAGAAGGC346240.37853758371798596No Hit
CAAAGAATTCTCCTTTTGGGCT306620.33522179389905515No Hit
TGAGGTAGTAGTTTGTACAGTT299790.3277546852553576No Hit
TAGCAGCACGTAAATATTGGC295770.3233596959804434No Hit
TGTCAGTTTGTCAAATACCCCAA283730.31019659377398384No Hit
TTCACAGTGGCTAAGTTCCG276660.3024670977108884No Hit
CTTCTCACTACTGCACTTGACTAG260290.28457008914612575No Hit
AGAATTACTCTGAGACCT258030.2820992742801292No Hit
CAACACCAGTCGATGGGCTGT256220.2801204358255036No Hit
TGTCAGTTTGTCAAATACCCCA253280.2769061899378798No Hit
CTCCCACTGCTTCACTTGACTAG240590.2630324551372177No Hit
CAACGGAATCCCAAAAGCAGCT237460.2596104858759039No Hit
TTCACAGTGGCTAAGTTCCGC232540.25423154377824764No Hit
TTCAAGTAATCCAGGATAGGC229690.251115693172898No Hit
TGATGTAGTAGGTTGTATAGTT229220.25060185114324385No Hit
TAGCTTATCAGACTGATGTTGACA209470.22900955308862792No Hit
CGCCTGTCTGAGCGTCGCT206000.22521586831650042No Hit
AACGGAATCCCAAAAGCAGCTG204760.22386019998294482No Hit
TTCACAGTGGCTAAGTTCTGC185330.20261775182085937No Hit
CCCCCCACTGCTAAATTTGACTGGCT182230.1992285809869703No Hit
AGCTCGGTCTGAGGCCCCTCAGT181340.19825556097336985No Hit
TGAGGTAGTAGTTTGTGCTGTT181310.19822276254594512No Hit
TCGTTTCCCGGCCAATGCACCA178680.19534743374171018No Hit
CAAAGAATTCTCCTTTTGGGCTT177060.1935763186607746No Hit
ATCACATTGCCAGGGATTTCC172890.1890173372487367No Hit
TTCAAGTAATCCAGGATAGGCTT167990.18366026076936365No Hit
CCCATAAAGTAGAAAGCACTA161320.17636807707193133No Hit
TGTAAACATCCCCGACTGGAAGC161250.17629154740794029No Hit
AACATTCAACGCTGTCGGTGAGTT160490.1754606539131804No Hit
TATTGCACTTGTCCCGGCCTGTT159820.17472815570069467No Hit
TTATAAAGCAATGAGACTGATT153530.1678514187506423No Hit
TGATGTAGTAGATTGTATAGTT150720.16477929938185895No Hit
TATTGCACTTGTCCCGGCCTGTAG145900.15950968537561852No Hit
TGTAAACATCCTACACTCAGCT144520.15800095771408082No Hit
TAGCAGCACGTAAATATTGGCGT144090.15753084692099298No Hit
CACCCGGCTGTGTGCACATGTGC141610.1548195102538817No Hit
GTCAGGATGGCCGAGCGGTCTAAGG140210.15328891697406083No Hit
ATCCCGGACGAGCCCCCA137790.150643177161799No Hit
TTCACAGTGGCTAAGTTCTG135670.14832542162378454No Hit
CACTAGATTGTGAGCTCCTGGAA132980.14538449596470016No Hit
TAGCAGCACATCATGGTTTACA132490.14484878831676284No Hit
CTACGCCTGTCTGAGCGTCGCT131790.1440834916768524No Hit
ATCACATTGCCAGGGATTTCCA129870.14198439232166948No Hit
ATGCACCTGGGCAAGGATTCTGA128480.14046473185099018No Hit
TGTCAGTTTGTCAAATACCCC128460.14044286623270702No Hit
CACTAGATTGTGAGCTCCTGGA125220.13690063607083586No Hit
AGCAGCATTGTACAGGGCTATGT123970.13553403492813865No Hit
TATTGCACTTGTCCCGGCCTGTAAA121940.13331467467239838No Hit
ATCCCACCGCTGCCACCA114330.12499480691565774No Hit
TTAATGCTAATCGTGATAGGGGTT112810.12333301992613795No Hit
ACTGGACTTGGAGTCAGAAGGCT111750.12217414215713071No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC107840.11789941378277383No Hit
TATTGCACTTGTCCCGGCCTGTAAT104070.11377774473639904No Hit
GGAATACCGGGTGCTGTAGGCTTT103870.11355908855356749No Hit
AGCTCTAGAATTACTCTGAGACCT102920.1125204716851176No Hit
CCCCCCACTGCTAAATTTGACTGG100790.11019178333796155No Hit
TCGTTTCCCGGGCGGCGCACCA100220.10956861321689162No Hit
CAAAGTGCTGTTCGTGCAGGT99920.1092406289426443No Hit
TGTCAGTTTGTCAAATACC98050.10719619363316928No Hit
TATTGCACTTGTCCCGGCCTGTTT97800.10692287340462983No Hit
AGCAGCATTGTACAGGGCTATG97700.10681354531321405No Hit
ACTGGACTTGGAGTCAGAAGGCA97140.10620130800128569No Hit
ACTGGACTTGGAGTCAGAAGG96920.10596078620017099No Hit
TGTAAACATCCTTGACTGGAAGC96510.10551254102536631No Hit
ACCCCCCACTGCTAAATTTGACTG95200.10408034302781961No Hit
CTCCCACTGCTTCACTTGACTAGCCT92910.10157672973439832No Hit
TGAGGTAGTAGGTTGTGTGGTT92740.10139087197899152No Hit
TGTAAACATCCCCGACTGGA92610.10124874546015102No Hit
CATAAAGTAGAAAGCACTAC92540.10117221579615995No Hit
AGAGGTAGTAGGTTGCATAGTT92470.10109568613216892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)