FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9995311
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG180126418.0210900891428No Hit
CCCCCCACTGCTAAATTTGACTG107881010.793160913152178No Hit
TTCAAGTAATCCAGGATAGGCT3844273.8460734238284333No Hit
CCCCCACTGCTAAATTTGACTG3415583.4171823167883417No Hit
AACATTCAACGCTGTCGGTGAG2667542.668791396285718No Hit
TAGCAGCACGTAAATATTGGCG2624652.62588127573019No Hit
AAGCTGCCAGTTGAAGAACTGT1932521.9334265837251088No Hit
TGAGGTAGTAGATTGTATAGTT1810581.8114293792359237No Hit
TATTGCACTTGTCCCGGCCTGTAA1554351.55507917662592No Hit
TCGTACGACTCTTAGCGG1489481.490178744813443No Hit
TAGCTTATCAGACTGATGTTGAC1342611.3432398451633971No Hit
TATTGCACTTGTCCCGGCCTGTAT1282861.2834618152451684No Hit
TAGCTTATCAGACTGATGTTGACT960240.9606904677603328No Hit
TATTGCACTTGTCCCGGCCTGT945120.9455633746663811No Hit
TGAGGTAGTAGGTTGTATAGTT873040.8734495604989179No Hit
CAACGGAATCCCAAAAGCAGCTGT826020.8264075024779118No Hit
TAGCTTATCAGACTGATGTTGA817540.8179235243405633No Hit
AACATTCAACGCTGTCGGTGAGT736780.7371256382117575No Hit
TGTAAACATCCTTGACTGGAAGCT733690.7340341886310492No Hit
TTCAAGTAATTCAGGATAGGTT680270.6805891282422328No Hit
TATTGCACTTGTCCCGGCCTGTA613830.6141179599114025No Hit
TGAGGTAGTAGTTTGTACAGTT560740.5610030543321763No Hit
CAACGGAATCCCAAAAGCAGCT555850.5561107603355213No Hit
CATAAAGTAGAAAGCACTACT550080.5503380535132923No Hit
AGCAGCATTGTACAGGGCTATGA545130.5453857313694391No Hit
TGAGAACTGAATTCCATAGGCTGT507730.5079681862825478No Hit
CATTGCACTTGTCTCGGTCTGA488680.4889092495471126No Hit
CTCCCACTGCTTCACTTGACTAG487920.4881488930159352No Hit
TTCTCACTACTGCACTTGACTAG466260.4664787318773773No Hit
CCCATAAAGTAGAAAGCACTAC398720.3989070475145796No Hit
AACGGAATCCCAAAAGCAGCTG394940.3951252742410916No Hit
TGTAAACATCCCCGACTGGAAGCT376160.3763364641680484No Hit
CTTCTCACTACTGCACTTGACTAG373880.374055394574516No Hit
CCCATAAAGTAGAAAGCACT371670.3718443578193815No Hit
TCGTTTCCCGGCCAATGCACCA353750.35391595118951275No Hit
CCCCCACTGCTAAATTTGACTGGCTT353360.3535257682327243No Hit
ACTGGACTTGGAGTCAGAAGGC348350.34851341794167284No Hit
ACCCCCCACTGCTAAATTTGACTG335390.33554733814685705No Hit
TTCACAGTGGCTAAGTTCCGC322380.32253123489604274No Hit
TGAGGTAGTAGTTTGTGCTGTT297440.2975795350439821No Hit
CTCCCACTGCTTCACTTGACTAGCCT294060.2941979494184823No Hit
TAGCAGCACGTAAATATTGGC282750.28288264367161764No Hit
TGAGAACTGAATTCCATGGGTT253240.25335879994129246No Hit
CAAAGAATTCTCCTTTTGGGCT242320.2424336771512162No Hit
CACCCGGCTGTGTGCACATGTGC236800.23691108760897986No Hit
CCCCCCACTGCTAAATTTGACTGG233860.23396970839626702No Hit
CACTAGATTGTGAGCTCCTGGA221160.2212637505726435No Hit
TGATGTAGTAGATTGTATAGTT219290.21939287331829896No Hit
TGTAAACATCCTACACTCTCAGCT218950.21905271381750901No Hit
CACTAGATTGTGAGCTCCTGGAA213490.21359015242247092No Hit
CCCATAAAGTAGAAAGCACTA208540.20863783027861765No Hit
GGAATACCGGGTGCTGTAGGCTTT199930.20002379115567293No Hit
CAACACCAGTCGATGGGCTGT194140.19423107495104455No Hit
TGAGGTAGTAGATTGTATAGT189090.18917870589519425No Hit
TTCACAGTGGCTAAGTTCTGC180250.18033455887465633No Hit
TCGTTTCCCGGCCAACGCACCA174510.1745918661260265No Hit
CCCCCCACTGCTAAATTTGACTGGCT170640.17072005063174123No Hit
TGTAAACATCCCCGACTGGA168660.16873912177419992No Hit
TATTGCACTTGTCCCGGCCTGTAG166490.16656810378386427No Hit
GCCTGTCTGAGCGTCGCT166000.16607787391507878No Hit
GGAATACCGGGTGCTGTAGGCTT163280.16335659790875942No Hit
TAGCTTATCAGACTGATGTTGACA160560.16063532190244006No Hit
AATCCTTGGAACCTAGGTGTGAGT158720.15879445872169462No Hit
AACCGGGCGGAAACACCA154450.15452245557942118No Hit
ATCACATTGCCAGGGATTTCC151910.1519812640146965No Hit
TGTCAGTTTGTCAAATACCCCA151060.15113086526272168No Hit
TCCCACTGCTTCACTTGACTAG147570.14763922803402516No Hit
TTCAAGTAATCCAGGATAGGC146150.14621856188366728No Hit
TTCAAGTAATCCAGGATAGGCTT142870.1429370231701645No Hit
TAGCAGCACATCATGGTTTACA141810.1418765259029959No Hit
CAAAGAATTCTCCTTTTGGGCTT141130.14119620690141607No Hit
AGCTCGGTCTGAGGCCCCTCAGT140610.14067596295903148No Hit
TATTGCACTTGTCCCGGCCTGTT137950.13801471509991034No Hit
TGGCTCAGTTCAGCAGGAAC133110.13317244455925384No Hit
CAAAGTGCTGTTCGTGCAGGTAG129990.13005098090494632No Hit
ATGCACCTGGGCAAGGATTCTGA129080.12914055400577332No Hit
AATGCACCCGGGCAAGGATTCT124800.12485854617230019No Hit
ACTGGACTTGGAGTCAGAAGGCT123410.1234678940955414No Hit
TATTGCACTTGTCCCGGCCTGTAAT122320.12237738275477372No Hit
AGCAGCATTGTACAGGGCTATGT118940.11899579712927391No Hit
TGAGGTAGTAGTTTGTGCTGTTA117650.11770519196451217No Hit
CAAAGAATTCTCCTTTTGGGC117280.11733501839012314No Hit
TTCACAGTGGCTAAGTTCTG113100.11315305746864704No Hit
TGTAAACATCCCCGACTGGAAGC107250.10730031311682049No Hit
TGATGTAGTAGGTTGTATAGTT105770.10581961881926435No Hit
TATTGCACTTGTCCCGGCCTGTAAA102200.1022479440609702No Hit
ACTGGACTTGGAGTCAGAAGGCA101150.10119745148500132No Hit
CAACGGAATCCCAAAAGCAGCTGA101030.10107739519060488No Hit
TAGCAGCACGTAAATATTGGCGT100910.10095733889620842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)