FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10675219
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9009138.43929290818296No Hit
CCCCCCACTGCTAAATTTGACTG6920466.48273351581827No Hit
TAGCAGCACGTAAATATTGGCG4888264.579072335658875No Hit
AAGCTGCCAGTTGAAGAACTGT4518994.233159057439477No Hit
AACATTCAACGCTGTCGGTGAGT4446934.1656569293800905No Hit
TTCAAGTAATCCAGGATAGGCT4243543.975131564045665No Hit
AACATTCAACGCTGTCGGTGAG2628342.462094688642922No Hit
CCCCCACTGCTAAATTTGACTG2416672.2638130421492995No Hit
TAGCTTATCAGACTGATGTTGAC1874031.755495601542226No Hit
TAGCTTATCAGACTGATGTTGACT1672741.5669374089655679No Hit
TGTAAACATCCTTGACTGGAAGCT1615051.512896363062903No Hit
TATTGCACTTGTCCCGGCCTGTAA1549361.451361325702077No Hit
TATTGCACTTGTCCCGGCCTGTAT1327191.2432438154196181No Hit
TAGCTTATCAGACTGATGTTGA1108661.0385360712506226No Hit
CATAAAGTAGAAAGCACTACT1078981.0107333629408446No Hit
TGAGGTAGTAGGTTGTATAGTT992300.9295359654916682No Hit
TATTGCACTTGTCCCGGCCTGT933270.8742396760197613No Hit
AGCAGCATTGTACAGGGCTATGA883300.827430331874222No Hit
TTCAAGTAATTCAGGATAGGTT735850.6893067018109886No Hit
TGTAAACATCCCCGACTGGAAGCT732860.6865058225035008No Hit
TATTGCACTTGTCCCGGCCTGTA663280.6213268317961439No Hit
TGAGAACTGAATTCCATAGGCTGT614100.575257519307098No Hit
TTAATGCTAATCGTGATAGGGGTT607330.5689157290356291No Hit
ACTGGACTTGGAGTCAGAAGGC599360.5614498400454361No Hit
CAACGGAATCCCAAAAGCAGCTGT589350.5520729832334118No Hit
TGTCAGTTTGTCAAATACCCCAA580970.544223027180988No Hit
TGAGGTAGTAGATTGTATAGTT566160.5303497754940671No Hit
TGTAAACATCCTACACTCTCAGCT556920.521694215359891No Hit
CCCATAAAGTAGAAAGCACTAC528560.4951280156407095No Hit
CGCCTGTCTGAGCGTCGCT448070.4197290940822853No Hit
CCCATAAAGTAGAAAGCACT444040.4159539958852367No Hit
CATTGCACTTGTCTCGGTCTGA394070.36914465173969735No Hit
TAGCAGCACGTAAATATTGGC340860.3193002410536027No Hit
TGTCAGTTTGTCAAATACCCCA335800.31456029145631575No Hit
TTCTCACTACTGCACTTGACTAG321810.30145517389385645No Hit
AACATTCAACGCTGTCGGTGAGTT319160.29897278922334053No Hit
CAAAGAATTCTCCTTTTGGGCT315400.2954506132380048No Hit
CTACGCCTGTCTGAGCGTCGCT290580.27220050473905966No Hit
CAACGGAATCCCAAAAGCAGCT280430.2626925030765177No Hit
CCCCCACTGCTAAATTTGACTGGCTT273840.2565193276128574No Hit
CTTCTCACTACTGCACTTGACTAG258360.24201845414131548No Hit
TGTAAACATCCCCGACTGGAAGC254930.2388054053036289No Hit
TGAGGTAGTAGTTTGTACAGTT251960.23602326097478657No Hit
CAAAGAATTCTCCTTTTGGGCTT249570.2337844310266609No Hit
TACGCCTGTCTGAGCGTCGCT234550.21971446206396328No Hit
TGTAAACATCCTACACTCAGCT233500.21873087568507962No Hit
TTCAAGTAATCCAGGATAGGCTT221940.2079020580280367No Hit
CAACACCAGTCGATGGGCTGT217300.20355554298230322No Hit
TAGCTTATCAGACTGATGTTGACA215860.2022066245198342No Hit
ACTGGACTTGGAGTCAGAAGGCA211120.19776643458087373No Hit
AACGGAATCCCAAAAGCAGCTG211020.1976727596876467No Hit
TCGTACGACTCTTAGCGG207350.19423489110621522No Hit
TTCACAGTGGCTAAGTTCCGC194580.18227260724112546No Hit
CCCCCCACTGCTAAATTTGACTGG189480.1774951876865477No Hit
TCGTTTCCCGGCCAATGCACCA189440.1774577177292569No Hit
TGAGAACTGAATTCCATGGGTT187580.1757153647152344No Hit
TAGCAGCACGTAAATATTGGCGT187430.17557485237539389No Hit
TGAGGTAGTAGTTTGTGCTGTT182510.17096604762862477No Hit
CTCCCACTGCTTCACTTGACTAG181070.16961712916615576No Hit
TTCAAGTAATCCAGGATAGGC177290.16607621820217458No Hit
TTCACAGTGGCTAAGTTCCG177090.16588886841572056No Hit
TATTGCACTTGTCCCGGCCTGTAG174170.16315356153349173No Hit
TATTGCACTTGTCCCGGCCTGTT171910.16103650894656119No Hit
ACCCCCCACTGCTAAATTTGACTG169960.1592098485286344No Hit
AGCAGCATTGTACAGGGCTATGT168560.1578984000234562No Hit
GCCTGTCTGAGCGTCGCT167380.15679303628337743No Hit
ATCACATTGCCAGGGATTTCC166750.15620288445604721No Hit
CCCCCCACTGCTAAATTTGACTGGCT165830.1553410754383587No Hit
ACTGGACTTGGAGTCAGAAGGCT164680.15426381416624801No Hit
CACTAGATTGTGAGCTCCTGGA164020.15364555987094972No Hit
CACTAGATTGTGAGCTCCTGGAA162240.15197814677150887No Hit
ATCACATTGCCAGGGATTTCCA151530.14194556570689557No Hit
TTATAAAGCAATGAGACTGATT149340.13989408554522395No Hit
ACCACAGGGTAGAACCACGGAC141890.13291530599981136No Hit
AACATTCAACGCTGTCGGTGA139620.1307888859235581No Hit
CAAAGTGCTGTTCGTGCAGGT138300.1295523773329615No Hit
AACATTCAACGCTGTCGGTGAGA135170.12662035317495593No Hit
TGTCAGTTTGTCAAATACCCC134910.1263767984525657No Hit
AACCGGGCGGAAACACCA134780.12625502109137057No Hit
TATTGCACTTGTCCCGGCCTGTAAT134430.12592715896507603No Hit
TGTAAACATCCTTGACTGGAAGC134020.1255430919028453No Hit
TTCACAGTGGCTAAGTTCTGC132700.12430658331224868No Hit
AGCTCGGTCTGAGGCCCCTCAGT132570.12418480595105356No Hit
GGAATACCGGGTGCTGTAGGCTTT131960.12361338910236877No Hit
ACTGGACTTGGAGTCAGAAGG129700.12149633651543823No Hit
TAGCAGCACATCATGGTTTACA128680.12054085260452267No Hit
AGCAGCATTGTACAGGGCTATG127750.11966967609751145No Hit
AATGACACGATCACTCCCGTTG126360.11836759508165592No Hit
ACGCCTGTCTGAGCGTCGCT124730.1168406943220556No Hit
CAAAGTGCTGTTCGTGCAGGTAG123590.11577280053926764No Hit
CCCATAAAGTAGAAAGCACTA121790.11408665246118135No Hit
CAGTGCAATAGTATTGTCAAAGC121180.11351523561249656No Hit
CTCCCACTGCTTCACTTGACTAGCCT121050.11339345825130144No Hit
TGAGGTAGTAGGTTGTGTGGTT121020.11336535578333334No Hit
CACCCGGCTGTGTGCACATGTGC120180.11257848668022642No Hit
CGCGACCTCAGATCAGACGTGGCG115250.10796031444413459No Hit
GGGCTACGCCTGTCTGAGCGTCGCT114440.10720154780899578No Hit
TGAGGTAGTAGGTTGTATAGT107370.10057873285784583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)