FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13745989
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTT9914617.212729473303085No Hit
CAACGGAATCCCAAAAGCAGCTG9566776.959681111340915No Hit
CCCCCCACTGCTAAATTTGACTG9127186.639886006019646No Hit
GGAATACCGGGTGCTGTAGGCTT6888615.01136004109999No Hit
AACATTCAACGCTGTCGGTGAGT4117322.9952882982810474No Hit
TTCAAGTAATCCAGGATAGGCT3955322.8774357377995865No Hit
AAGCTGCCAGTTGAAGAACTGT3828922.785481641226397No Hit
TAGCAGCACGTAAATATTGGCG3458162.5157593244109244No Hit
AACATTCAACGCTGTCGGTGAG2947112.1439781451883895No Hit
CCCCCACTGCTAAATTTGACTG2749051.9998924777256843No Hit
TGAATACCGGGTGCTGTAGGCTTT2339781.7021547158229213No Hit
TATTGCACTTGTCCCGGCCTGTAA2209151.6071233579482713No Hit
TATTGCACTTGTCCCGGCCTGTAT2184131.5889216847183567No Hit
GGAATACCGGGTGCTGTAGGCTTA1856201.3503575479363472No Hit
TGAATACCGGGTGCTGTAGGCTT1608761.170348674075034No Hit
TAGCTTATCAGACTGATGTTGACT1579041.1487278216212744No Hit
TAGCTTATCAGACTGATGTTGAC1530981.1137648953451076No Hit
TATTGCACTTGTCCCGGCCTGT1348450.9809770690199155No Hit
TATTGCACTTGTCCCGGCCTGTA1098540.7991713073537305No Hit
CATAAAGTAGAAAGCACTACT1084690.789095640917507No Hit
GGAATACCGGGTGCTGTAGGCT1067050.7762628065539701No Hit
TAGCTTATCAGACTGATGTTGA1066080.7755571461609638No Hit
TGTAAACATCCTTGACTGGAAGCT984570.716259848600199No Hit
TGAGGTAGTAGGTTGTATAGTT823950.5994112173376539No Hit
AGCAGCATTGTACAGGGCTATGA726070.5282049912887316No Hit
TCGTACGACTCTTAGCGG721660.5249967826978474No Hit
CCCATAAAGTAGAAAGCACTAC702660.5111745688142192No Hit
TGAGGTAGTAGATTGTATAGTT627210.45628582999739054No Hit
TTCAAGTAATTCAGGATAGGTT621610.4522119143264264No Hit
TGTAAACATCCCCGACTGGAAGCT600790.43706567784973493No Hit
TTAATGCTAATCGTGATAGGGGTT592340.4309184300962266No Hit
ATCCCGGACGAGCCCCCA578320.4207190912199915No Hit
CAACGGAATCCCAAAAGCAGCTGT556610.4049253931455932No Hit
ACTGGACTTGGAGTCAGAAGGC551100.40091695111934106No Hit
CCCATAAAGTAGAAAGCACT527530.3837701310542297No Hit
CATTGCACTTGTCTCGGTCTGA487630.3547434818986106No Hit
TGAATACCGGGTGCTGTAGGCTTA446970.3251639441876463No Hit
TGTAAACATCCTACACTCTCAGCT409500.2979050834392491No Hit
CAACGGAATCCCAAAAGCAGCT393380.2861780261864024No Hit
CAAAGAATTCTCCTTTTGGGCT375510.2731778702863795No Hit
CAACACCAGTCGATGGGCTGT340050.24738125426988192No Hit
CACTAGATTGTGAGCTCCTGGA312890.22762276326570607No Hit
CTCCCACTGCTTCACTTGACTAG308860.22469099895249442No Hit
CGCCTGTCTGAGCGTCGCT307230.22350519849826742No Hit
AACATTCAACGCTGTCGGTGAGTT304060.22119907123452523No Hit
TTCTCACTACTGCACTTGACTAG298910.2174525237871207No Hit
GGAATACCGGGTGCTGTAGGCTA298050.21682688673765127No Hit
TTCACAGTGGCTAAGTTCCG296790.21591025571168435No Hit
CACTAGATTGTGAGCTCCTGGAA289150.21035227076058333No Hit
CTTCTCACTACTGCACTTGACTAG277320.2017461239056717No Hit
TGAGAACTGAATTCCATAGGCTGT275050.2000947330890487No Hit
TATTGCACTTGTCCCGGCCTGTT274380.19960731817841557No Hit
TATTGCACTTGTCCCGGCCTGTAG273840.199214476310144No Hit
TGTCAGTTTGTCAAATACCCCAA264810.1926452872907144No Hit
TGAATACCGGGTGCTGTAGGCT251870.1832316321510224No Hit
TGAGGTAGTAGTTTGTACAGTT236750.17223205983941933No Hit
GCCTGTCTGAGCGTCGCT233710.17002050561803883No Hit
ACCGGGTGCTGTAGGCTTA225810.1642733745822145No Hit
ACCCCCCACTGCTAAATTTGACTG220010.1600539619230017No Hit
CAAAGAATTCTCCTTTTGGGCTT215300.15662750784974439No Hit
TCGTTTCCCGGCCAATGCACCA214750.1562273911320604No Hit
CCCCCACTGCTAAATTTGACTGGCTT214340.15592912230615055No Hit
TAGCAGCACGTAAATATTGGC212460.1545614506166126No Hit
AACCGGGCGGAAACACCA210880.15341202440944773No Hit
CTACGCCTGTCTGAGCGTCGCT209750.15258996642584247No Hit
AACGGAATCCCAAAAGCAGCTG209610.15248811853406838No Hit
CCCATAAAGTAGAAAGCACTA204080.14846512680899135No Hit
CGAATACCGGGTGCTGTAGGCTTT202280.14715565391475288No Hit
AGCTACATCTGGCTACTGGGTCTC199820.14536604095929365No Hit
TTCAAGTAATCCAGGATAGGCTT198160.1441584159568293No Hit
AGCTCGGTCTGAGGCCCCTCAGT197730.14384559743209455No Hit
TGTCAGTTTGTCAAATACCCCA192690.14017907332822688No Hit
TATTGCACTTGTCCCGGCCTGTTT191220.1391096704645988No Hit
ATACCGGGTGCTGTAGGCTTA189160.13761105148563701No Hit
ACTGGACTTGGAGTCAGAAGGCA186660.13579233913252803No Hit
TCGTTTCCCGGGCGGCGCACCA186130.13540677211366894No Hit
CCCCCCACTGCTAAATTTGACTGG185220.13474476081713727No Hit
TTCAAGTAATCCAGGATAGGC184020.13387177888764498No Hit
TGAGGTAGTAGTTTGTGCTGTT181340.13192211924511216No Hit
ACTGGACTTGGAGTCAGAAGGCT172510.12549842721393129No Hit
CCCCCCACTGCTAAATTTGACTGGCT171300.12461817043502654No Hit
TGTAAACATCCCCGACTGGAAGC170440.12399253338555706No Hit
TATTGCACTTGTCCCGGCCTGTAAT170220.12383248669848347No Hit
ACCGGGTGCTGTAGGCTT164770.11986769376870592No Hit
CACCCGGCTGTGTGCACATGTGC163830.11918385792393693No Hit
TAGCTTATCAGACTGATGTTGACA162430.11816537900619592No Hit
TTCACAGTGGCTAAGTTCTG158340.11518996559650965No Hit
GTAATACCGGGTGCTGTAGGCTTT157740.11475347463176348No Hit
TTCACAGTGGCTAAGTTCCGC153560.11171258757736528No Hit
TATTGCACTTGTCCCGGCCTGA152410.11087597989493517No Hit
AGCAGCATTGTACAGGGCTATGT151870.11048313802666362No Hit
ACCGGGTGCTGTAGGCTTT149130.10848982928765621No Hit
TACGCCTGTCTGAGCGTCGCT146310.10643832175334929No Hit
TGAGAACTGAATTCCATGGGTT145930.10616187747567672No Hit
ATACCGGGTGCTGTAGGCTTT143940.10471418244260197No Hit
TTAATACCGGGTGCTGTAGGCTTT143800.10461233455082788No Hit
TTCACAGTGGCTAAGTTCTGC141830.10317918921657801No Hit
ACTGGACTTGGAGTCAGAAGG141490.1029318443365552No Hit
TGTAAACATCCTACACTCAGCT141410.10287364554125569No Hit
CAAAGTGCTGTTCGTGCAGGT140980.10256082701652096No Hit
ACCACAGGGTAGAACCACGGAC140670.10233530668473545No Hit
CGAATACCGGGTGCTGTAGGCTT139790.10169511993644109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)