FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9403021
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7496567.972501603474032No Hit
CCCCCCACTGCTAAATTTGACTG6336556.738844888254531No Hit
AACATTCAACGCTGTCGGTGAGT3863644.108934777450779No Hit
AAGCTGCCAGTTGAAGAACTGT3555993.7817526941607387No Hit
TTCAAGTAATCCAGGATAGGCT3407343.6236652029172327No Hit
TAGCAGCACGTAAATATTGGCG3216663.420879311021426No Hit
AACATTCAACGCTGTCGGTGAG2279692.4244229593872015No Hit
CCCCCACTGCTAAATTTGACTG2083572.215851692769802No Hit
TAGCTTATCAGACTGATGTTGAC1341091.4262331223124993No Hit
TATTGCACTTGTCCCGGCCTGTAA1192691.2684115030690668No Hit
TAGCTTATCAGACTGATGTTGACT1094741.1642428534403997No Hit
TATTGCACTTGTCCCGGCCTGTAT1036671.102486105263404No Hit
TGAGGTAGTAGGTTGTATAGTT963711.024894020762051No Hit
CGCCTGTCTGAGCGTCGCT961631.022681965721442No Hit
TATTGCACTTGTCCCGGCCTGT944811.0047940975565193No Hit
TGTAAACATCCTTGACTGGAAGCT929640.9886609845920794No Hit
TAGCTTATCAGACTGATGTTGA899610.9567244399432906No Hit
CATAAAGTAGAAAGCACTACT856260.9106222351306031No Hit
GCCTGTCTGAGCGTCGCT848000.9018378242481858No Hit
AGCAGCATTGTACAGGGCTATGA703550.7482169826059093No Hit
TATTGCACTTGTCCCGGCCTGTA627140.6669558645035463No Hit
TGAGGTAGTAGATTGTATAGTT579300.6160785985695448No Hit
TTCAAGTAATTCAGGATAGGTT543080.5775590631989442No Hit
TGTAAACATCCCCGACTGGAAGCT483590.5142921620615332No Hit
CCCATAAAGTAGAAAGCACTAC461820.4911400282951618No Hit
ACTGGACTTGGAGTCAGAAGGC453340.4821216500526799No Hit
CAACGGAATCCCAAAAGCAGCTGT426610.4536946158048567No Hit
TGTCAGTTTGTCAAATACCCCAA369590.39305453002816865No Hit
CATTGCACTTGTCTCGGTCTGA368050.3914167585077179No Hit
CCCATAAAGTAGAAAGCACT327290.3480689876157886No Hit
TCGTACGACTCTTAGCGG313050.3329249184916209No Hit
AACATTCAACGCTGTCGGTGAGTT306040.3254698676095693No Hit
TGTAAACATCCTACACTCTCAGCT303500.3227686080888259No Hit
TTCTCACTACTGCACTTGACTAG301650.32080115528828446No Hit
AACCGGGCGGAAACACCA295200.31394165768639676No Hit
CTACGCCTGTCTGAGCGTCGCT283140.3011159924028671No Hit
CAACACCAGTCGATGGGCTGT280690.29851044680215005No Hit
TACGCCTGTCTGAGCGTCGCT275300.2927782464805726No Hit
TTCACAGTGGCTAAGTTCCG262760.27944210695690247No Hit
CTCCCACTGCTTCACTTGACTAG247630.2633515335124743No Hit
CTTCTCACTACTGCACTTGACTAG235470.25041951942891544No Hit
TGAGGTAGTAGTTTGTACAGTT228900.24343240326699261No Hit
ACGCCTGTCTGAGCGTCGCT226910.24131606214641016No Hit
TAGCAGCACGTAAATATTGGC225590.23991225798602384No Hit
TGTCAGTTTGTCAAATACCCCA220890.23491386438464829No Hit
GCGTTCGCGCTTTCCCCTG208930.22219454790114793No Hit
CCCCCACTGCTAAATTTGACTGGCTT192670.20490223301638907No Hit
ACCCCCCACTGCTAAATTTGACTG190060.2021265293356252No Hit
CAAAGAATTCTCCTTTTGGGCT175540.18668468357137563No Hit
TTCAAGTAATCCAGGATAGGC174590.1856743699710976No Hit
CAACGGAATCCCAAAAGCAGCT174070.18512135621094541No Hit
ATCCCACCGCTGCCACCA173310.18431310533072298No Hit
TGAGGTAGTAGTTTGTGCTGTT173220.18421739141069662No Hit
TAGCAGCACGTAAATATTGGCGT172900.18387707525060298No Hit
TTCACAGTGGCTAAGTTCCGC169120.17985709060949667No Hit
TCGTTTCCCGGCCAATGCACCA168170.17884677700921864No Hit
TGATGTAGTAGGTTGTATAGTT167930.1785915398891484No Hit
TTCAAGTAATCCAGGATAGGCTT167510.17814487492902548No Hit
GGAATACCGGGTGCTGTAGGCTTT165380.17587964548840207No Hit
TGTAAACATCCTACACTCAGCT163900.17430568324796894No Hit
AACGGAATCCCAAAAGCAGCTG162500.17281680004755917No Hit
CCCCCCACTGCTAAATTTGACTGG161320.17156188420721383No Hit
ACCGGGTGCTGTAGGCTT161030.17125347268712895No Hit
TATTGCACTTGTCCCGGCCTGTT160610.17080680772700602No Hit
ATCCCGGACGAGCCCCCA157160.1671377741259963No Hit
TGTAAACATCCCCGACTGGAAGC148980.15843844228360227No Hit
TAGCTTATCAGACTGATGTTGACA147030.15636464068303155No Hit
TTATAAAGCAATGAGACTGATT146450.1557478176428618No Hit
CAAAGTGCTGTTCGTGCAGGT144770.1539611578023701No Hit
AGCAGCATTGTACAGGGCTATGT141720.15071751940147746No Hit
TTCACAGTGGCTAAGTTCTGC140630.14955831748115844No Hit
CCCCCCACTGCTAAATTTGACTGGCT139250.14809070404075456No Hit
GGAATACCGGGTGCTGTAGGCTT138390.14717610436050288No Hit
ACTGGACTTGGAGTCAGAAGGCA134760.14331564291944046No Hit
ACCACAGGGTAGAACCACGGAC132710.1411354925188405No Hit
AGCAGCATTGTACAGGGCTATG132470.14088025539877025No Hit
TTCACAGTGGCTAAGTTCTG127470.13556281539730689No Hit
TATTGCACTTGTCCCGGCCTGTAG126690.1347332947570786No Hit
TGAGGTAGTAGGTTGTATAGT125920.13391440899685325No Hit
CAAAGAATTCTCCTTTTGGGCTT124490.13239362115643472No Hit
ACTGGACTTGGAGTCAGAAGG117120.12455571459427774No Hit
CTCCCACTGCTTCACTTGACTAGCCT116230.12360921027401725No Hit
AGCTCGGTCTGAGGCCCCTCAGT116200.12357730563400848No Hit
TGAGGTAGTAGGTTGTGTGGTT114670.12195016899356069No Hit
ACTGGACTTGGAGTCAGAAGGCT113810.121035569313309No Hit
CAAAGTGCTGTTCGTGCAGGTAG113010.12018477891307484No Hit
AACATTCAACGCTGTCGGTGAGA112310.11944033731286997No Hit
AACATTCAACGCTGTCGGTGA111740.11883414915270316No Hit
GGGCTACGCCTGTCTGAGCGTCGCT110480.1174941542723344No Hit
ATCACATTGCCAGGGATTTCC110480.1174941542723344No Hit
TAGCAGCACATCATGGTTTACA109710.11667526851210903No Hit
CACTAGATTGTGAGCTCCTGGA107060.11385702531133346No Hit
ACCGGGTGCTGTAGGCTTA104370.11099624259054616No Hit
ACCGGGTGCTGTAGGCTTT103720.11030497539035591No Hit
AGAGGTAGTAGGTTGCATAGTT100700.10709324162947206No Hit
CACCCGGCTGTGTGCACATGTGC99340.10564689794907403No Hit
TGATGTAGTAGATTGTATAGTT98170.1044026169887316No Hit
CACTAGATTGTGAGCTCCTGGAA96410.10253087810821651No Hit
ATCACATTGCCAGGGATTTCCA96230.10233945026816382No Hit
CCCATAAAGTAGAAAGCACTA96150.1022543712281404No Hit
TTCACCACCTTCTCCACCCAGC94360.10035072770761652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)