FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14617485
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG12804808.759920054646884No Hit
CAACGGAATCCCAAAAGCAGCTG11137237.619115052965678No Hit
AAGCTGCCAGTTGAAGAACTGT6655084.552821501099539No Hit
TAGCAGCACGTAAATATTGGCG6043374.134343219780968No Hit
TTCAAGTAATCCAGGATAGGCT5211603.565319205047927No Hit
AACATTCAACGCTGTCGGTGAGT4230592.894198283767693No Hit
CCCCCACTGCTAAATTTGACTG3376422.309850155481603No Hit
TAGCTTATCAGACTGATGTTGAC3357822.2971256683348744No Hit
TAGCTTATCAGACTGATGTTGA2793551.911101670362583No Hit
TAGCTTATCAGACTGATGTTGACT2464451.6859603413309472No Hit
AACATTCAACGCTGTCGGTGAG2405741.645796113353289No Hit
TATTGCACTTGTCCCGGCCTGT1957531.339170178727736No Hit
TATTGCACTTGTCCCGGCCTGTAT1951861.3352912624846203No Hit
TATTGCACTTGTCCCGGCCTGTAA1870661.2797413508548154No Hit
TGAGGTAGTAGGTTGTATAGTT1657041.133601300086848No Hit
AGCAGCATTGTACAGGGCTATGA1426480.975872388444387No Hit
TGTAAACATCCTTGACTGGAAGCT1409340.9641467051274553No Hit
CATAAAGTAGAAAGCACTACT1182970.809284223654069No Hit
TCGTACGACTCTTAGCGG1152630.7885282591362331No Hit
TATTGCACTTGTCCCGGCCTGTA1078230.7376303105493182No Hit
TGAGGTAGTAGATTGTATAGTT974820.6668862666867795No Hit
TTCAAGTAATTCAGGATAGGTT949970.6498860782138651No Hit
ACTGGACTTGGAGTCAGAAGGC894420.6118836448267263No Hit
TGTAAACATCCCCGACTGGAAGCT854340.5844644273621625No Hit
GCCTGTCTGAGCGTCGCT848970.5807907447827036No Hit
CGCCTGTCTGAGCGTCGCT787340.5386289091454515No Hit
CAACGGAATCCCAAAAGCAGCTGT729110.49879305502964427No Hit
TGTAAACATCCTACACTCTCAGCT723520.4949688677635038No Hit
CTCCCACTGCTTCACTTGACTAG665660.4553861351662068No Hit
CATTGCACTTGTCTCGGTCTGA638570.4368535353379874No Hit
CCCATAAAGTAGAAAGCACTAC611230.4181499074567205No Hit
CCCATAAAGTAGAAAGCACT535390.36626683728425236No Hit
CTTCTCACTACTGCACTTGACTAG450930.3084867198427089No Hit
TTCTCACTACTGCACTTGACTAG445370.3046830559429341No Hit
GCGTTCGCGCTTTCCCCTG389170.266235949617872No Hit
CAACGGAATCCCAAAAGCAGCT383660.2624664913287067No Hit
TGAGGTAGTAGTTTGTACAGTT362110.24772387315601826No Hit
CAACACCAGTCGATGGGCTGT360580.24667718147136802No Hit
TGAGGTAGTAGTTTGTGCTGTT357450.24453591024721422No Hit
AGCAGCATTGTACAGGGCTATGT354280.24236727453457282No Hit
TTCAAGTAATCCAGGATAGGCTT346690.23717486284405287No Hit
AACATTCAACGCTGTCGGTGAGTT345290.2362171057469873No Hit
TAGCTTATCAGACTGATGTTGACA342140.23406215227858965No Hit
CTACGCCTGTCTGAGCGTCGCT333150.22791198349100408No Hit
AGCTCGGTCTGAGGCCCCTCAGT330550.22613329173931082No Hit
TGTAAACATCCTACACTCAGCT323330.2211940015673011No Hit
CCCCCCACTGCTAAATTTGACTGG319530.21859437516098013No Hit
TACGCCTGTCTGAGCGTCGCT314570.21520117858851917No Hit
TATTGCACTTGTCCCGGCCTGTT313380.2143870850560134No Hit
TTCACAGTGGCTAAGTTCCG303720.20777856108626075No Hit
CACCCGGCTGTGTGCACATGTGC292500.20010282206549213No Hit
CAAAGAATTCTCCTTTTGGGCT288970.1976879059564624No Hit
TAGCAGCACGTAAATATTGGC283280.19379530746910292No Hit
AGCAGCATTGTACAGGGCTATG276400.1890886154492377No Hit
CACTAGATTGTGAGCTCCTGGA272410.18635900772260072No Hit
AACGGAATCCCAAAAGCAGCTG271890.18600326937226205No Hit
ACCACAGGGTAGAACCACGGAC266290.18217224098399964No Hit
TGTAAACATCCCCGACTGGAAGC264500.18094768012418005No Hit
CCCCCCACTGCTAAATTTGACTGGCT258350.1767403900192133No Hit
TTCAAGTAATCCAGGATAGGC251740.17221840829663929No Hit
TGATGTAGTAGGTTGTATAGTT246710.16877732386932498No Hit
TTATAAAGCAATGAGACTGATT244850.16750487515465212No Hit
TAGCAGCACGTAAATATTGGCGT243300.16644450122575807No Hit
CAAAGAATTCTCCTTTTGGGCTT241210.16501470670228155No Hit
TATTGCACTTGTCCCGGCCTGTAG224880.15384315427722348No Hit
CACTAGATTGTGAGCTCCTGGAA221360.1514350792903157No Hit
TGAGGTAGTAGGTTGTGTGGTT219630.15025156516322746No Hit
CAAAGTGCTGTTCGTGCAGGTAG216390.1480350415957328No Hit
ACGCCTGTCTGAGCGTCGCT211330.14457343380205281No Hit
ACCCCCCACTGCTAAATTTGACTG208580.14269212521853109No Hit
CCCCCACTGCTAAATTTGACTGGCTT208560.1426784429742873No Hit
AATGACACGATCACTCCCGTTG199380.13639829286638572No Hit
TATTGCACTTGTCCCGGCCTGTAC197850.13535160118173542No Hit
AACCGGGCGGAAACACCA194800.13326505893455678No Hit
TGTCAGTTTGTCAAATACCCCAA191750.13117851668737818No Hit
CAAAGTGCTGTTCGTGCAGGT187100.12799739490069598No Hit
CTCCCACTGCTTCACTTGACTAGCCT184580.12627343212597789No Hit
CCCATAAAGTAGAAAGCACTA181340.12405690855848321No Hit
TATTGCACTTGTCCCGGCCTGA175600.12013010446051424No Hit
GGGCTACGCCTGTCTGAGCGTCGCT175560.12010273997202665No Hit
TCGTTTCCCGGGCGGCGCACCA173560.11873451554764722No Hit
TGTCAGTTTGTCAAATACCCCA171670.11744154346660866No Hit
TCGTTTCCCGGCCAATGCACCA170160.11640853402620217No Hit
GGAATACCGGGTGCTGTAGGCTTT162710.1113118980453888No Hit
TATTGCACTTGTCCCGGCCTGTTT162690.111298215801145No Hit
TTCACAGTGGCTAAGTTCTGC161670.11060042134471149No Hit
ACTGGACTTGGAGTCAGAAGG161520.11049780451288303No Hit
ATCCCACCGCTGCCACCA159140.1088696174478715No Hit
TGAGGTAGTAGGTTGTATAGT158620.10851387909753285No Hit
TAGCTTATCAGACTGATGTTG154720.10584584146999296No Hit
CAACGGAATCCCAAAAGCAGCTGA154510.10570217790543313No Hit
ACTGGACTTGGAGTCAGAAGGCA154250.10552430873026379No Hit
ATCCCGGACGAGCCCCCA153990.10534643955509447No Hit
TTCACAGTGGCTAAGTTCCGC152890.10459391612168578No Hit
ATCACATTGCCAGGGATTTCCA150080.10267156080543269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)