FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6795723
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT121956617.946081675194826No Hit
CAACGGAATCCCAAAAGCAGCTG4709066.929446653431872No Hit
CCCCCCACTGCTAAATTTGACTG3445915.0707040295786046No Hit
TTCAAGTAATCCAGGATAGGCT2118283.117078197566322No Hit
AAGCTGCCAGTTGAAGAACTGT2051963.0194874040628203No Hit
TAGCAGCACGTAAATATTGGCG1948172.8667589894408585No Hit
AACATTCAACGCTGTCGGTGAGT1635912.4072640983159554No Hit
CCCCCACTGCTAAATTTGACTG1311001.9291545579476974No Hit
AACATTCAACGCTGTCGGTGAG1268621.8667918041980227No Hit
TAGCTTATCAGACTGATGTTGA870521.2809821706976579No Hit
TGAGGTAGTAGGTTGTATAGTT847441.2470196327896237No Hit
TAGCTTATCAGACTGATGTTGAC833321.2262418582982266No Hit
TATTGCACTTGTCCCGGCCTGT694781.0223783400235709No Hit
TAGCTTATCAGACTGATGTTGACT675370.9938162576667707No Hit
TATTGCACTTGTCCCGGCCTGTAA628260.9244932437652329No Hit
TGAGGTAGTAGATTGTATAGTT576350.8481069637476395No Hit
CATAAAGTAGAAAGCACTACT571860.8414998669015791No Hit
TATTGCACTTGTCCCGGCCTGTAT558840.8223407575617782No Hit
TTCAAGTAATTCAGGATAGGTT486890.7164653415096525No Hit
TCGTACGACTCTTAGCGG451780.6648004928982538No Hit
AACCGGGCGGAAACACCA391790.5765243815853001No Hit
TGTAAACATCCTTGACTGGAAGCT377540.5555553103032599No Hit
CAACGGAATCCCAAAAGCAGCT373110.5490365042836501No Hit
TATTGCACTTGTCCCGGCCTGTA350330.5155154205078694No Hit
TCCTGTCTGAGCGTCGCT332320.489013457434919No Hit
AGCAGCATTGTACAGGGCTATGA301580.44377912401667935No Hit
CGCCTGTCTGAGCGTCGCT297110.4372014574461025No Hit
CAACGGAATCCCAAAAGCAGCTGT293920.4325073285064739No Hit
CCCATAAAGTAGAAAGCACT269290.39626394424846334No Hit
CTCCCACTGCTTCACTTGACTAG253170.37254314220870977No Hit
TGAGAACTGAATTCCATGGGTT242960.3575189865743498No Hit
TTAATGCTAATCGTGATAGGGGTT241250.35500269802050494No Hit
CCCATAAAGTAGAAAGCACTAC240310.3536194750727774No Hit
CATTGCACTTGTCTCGGTCTGA238050.3502938539431345No Hit
TGAGGTAGTAGTTTGTACAGTT232070.34149420157354854No Hit
TGTAAACATCCCCGACTGGAAGCT209750.3086500141338898No Hit
ACTGGACTTGGAGTCAGAAGGC209320.30801726321099315No Hit
CAACACCAGTCGATGGGCTGT203780.2998650769020456No Hit
TTCTCACTACTGCACTTGACTAG181120.26652057477916624No Hit
GGAATACCGGGTGCTGTAGGCTTT179440.26404843163854674No Hit
TGAGGTAGTAGTTTGTGCTGTT164190.2416078465823283No Hit
TGTAAACATCCTACACTCTCAGCT152000.22367009367509536No Hit
AACGGAATCCCAAAAGCAGCTG146500.21557676791711494No Hit
ATCCCGGACGAGCCCCCA142580.20980843392233617No Hit
CACCCGTAGAACCGACCTTGCG142130.20914625272395593No Hit
GGAATACCGGGTGCTGTAGGCTT140870.2072921453684913No Hit
TAGCAGCACGTAAATATTGGC138240.20342206414240252No Hit
TCGTTTCCCGGCCAATGCACCA137380.20215656229660922No Hit
CACCCGGCTGTGTGCACATGTGC135780.19980214025792398No Hit
GCCTGTCTGAGCGTCGCTT118160.17387406755690307No Hit
TGAGGTAGTAGGTTGTGTGGTT113710.16732583126180983No Hit
CTACGCCTGTCTGAGCGTCGCT110920.16322030783185246No Hit
TTCAAGTAATCCAGGATAGGC110620.162778853699599No Hit
TTCACAGTGGCTAAGTTCCG107030.15749611925029905No Hit
AGCTCGGTCTGAGGCCCCTCAGT105020.15453837656420075No Hit
CCCCCACTGCTAAATTTGACTGGCTT104570.15387619536582053No Hit
TATTGCACTTGTCCCGGCCTGTT104210.15334645040711636No Hit
TAGCTTATCAGACTGATGTTGACA102440.15074187102682082No Hit
CTTCTCACTACTGCACTTGACTAG101670.14960880542070357No Hit
TGAGAACTGAATTCCATAGGCTGT101580.14947636918102752No Hit
ACCGGGTGCTGTAGGCTT98220.14453208289978858No Hit
TTCAAGTAATCCAGGATAGGCTT95180.14005868102628669No Hit
CAACGGAATCCCAAAAGCAGCTGA93750.1379544163292118No Hit
AATGACACGATCACTCCCGTTG91010.13392246858796333No Hit
TGAGGTAGTAGGTTGTATAGT90990.13389303831247978No Hit
GCCTGTCTGAGCGTCGCA89980.13240680940055974No Hit
TTCACAGTGGCTAAGTTCCGC89380.13152390113605278No Hit
TTCACAGTGGCTAAGTTCTGC87190.1283012859706024No Hit
AATGCACCCGGGCAAGGATTCT86370.12709464467577622No Hit
AACATTCAACGCTGTCGGTGAGTT85940.12646189375287956No Hit
CCCCCCACTGCTAAATTTGACTGG85250.12544654924869655No Hit
TACGCCTGTCTGAGCGTCGCT84740.12469607722386565No Hit
CAAAGAATTCTCCTTTTGGGCT84200.12390145978580941No Hit
CACTAGATTGTGAGCTCCTGGA80590.11858929506102589No Hit
GCCTGTCTGAGCGTCGCG78650.11573455833912005No Hit
TGATGTAGTAGGTTGTATAGTT77510.11405703263655685No Hit
CAAAGTGCTGTTCGTGCAGGT76980.11327713033624236No Hit
ACCGGGTGCTGTAGGCTTT74230.10923046745725216No Hit
GCCGGTCTGAGCGTCGCT74150.1091127463553179No Hit
CTCCCACTGCTTCACTTGACTAGCCT73920.1087742981872569No Hit
TTATAAAGCAATGAGACTGATT73490.10814154726436025No Hit
TGTCAGTTTGTCAAATACCCCAA73130.10761180230565608No Hit
TATTGCACTTGTCCCGGCCTGTAG71740.1055663981595483No Hit
CCCATAAAGTAGAAAGCACTA71610.10537510136890511No Hit
CCCCCCACTGCTAAATTTGACTGGCT71350.10499250778761876No Hit
ACGCCTGTCTGAGCGTCGCT70800.10418317521182073No Hit
TCCCACTGCTTCACTTGACTAG70770.10413902979859538No Hit
TTCACAGTGGCTAAGTTCTG70550.10381529676827617No Hit
ATCCCACCGCTGCCACCA70550.10381529676827617No Hit
TGTCAGTTTGTCAAATACCCCA69640.10247621923377395No Hit
AGCAGCATTGTACAGGGCTATGT68410.10066625729153468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)