FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9710463
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8465468.717874729557181No Hit
CCCCCCACTGCTAAATTTGACTG5871486.0465499945780135No Hit
AAGCTGCCAGTTGAAGAACTGT4725974.866884308194161No Hit
TAGCAGCACGTAAATATTGGCG3929994.047170562309954No Hit
TTCAAGTAATCCAGGATAGGCT3842983.957566184022327No Hit
AACATTCAACGCTGTCGGTGAGT2602932.6805415972441273No Hit
CCCCCACTGCTAAATTTGACTG2181172.2462059739067026No Hit
TAGCTTATCAGACTGATGTTGAC2151092.215229078160331No Hit
AACATTCAACGCTGTCGGTGAG1893151.9495980778671418No Hit
GCCTGTCTGAGCGTCGCT1859621.9150683134264557No Hit
TAGCTTATCAGACTGATGTTGACT1793411.846884129006001No Hit
TAGCTTATCAGACTGATGTTGA1760181.8126633096691682No Hit
TGAGGTAGTAGGTTGTATAGTT1640541.6894560022524159No Hit
CATAAAGTAGAAAGCACTACT1220151.2565312282225884No Hit
TATTGCACTTGTCCCGGCCTGTAA1142151.1762055012206936No Hit
TGTAAACATCCTTGACTGGAAGCT1120131.153528930597851No Hit
TGAGGTAGTAGATTGTATAGTT983801.0131339772367187No Hit
TATTGCACTTGTCCCGGCCTGTAT931310.959078882232495No Hit
TATTGCACTTGTCCCGGCCTGT781130.8044209632434622No Hit
TCCTGTCTGAGCGTCGCT772280.7953070826797857No Hit
AGCAGCATTGTACAGGGCTATGA618680.6371271895068237No Hit
CAACGGAATCCCAAAAGCAGCTGT580710.5980250375291065No Hit
TTCAAGTAATTCAGGATAGGTT560370.5770785594878431No Hit
TATTGCACTTGTCCCGGCCTGTA531470.5473168478166283No Hit
TGTAAACATCCCCGACTGGAAGCT516370.5317666109226717No Hit
CCCATAAAGTAGAAAGCACTAC469460.4834578948501219No Hit
GGAATACCGGGTGCTGTAGGCTTT420130.4326570216064878No Hit
CCCATAAAGTAGAAAGCACT413870.42621036710607924No Hit
TGAGGTAGTAGTTTGTACAGTT410640.4228840581545905No Hit
TTAATGCTAATCGTGATAGGGGTT410640.4228840581545905No Hit
ACTGGACTTGGAGTCAGAAGGC408040.4202065339211941No Hit
CAACGGAATCCCAAAAGCAGCT395170.40695278896588144No Hit
TGTAAACATCCTACACTCTCAGCT392240.40393542511824615No Hit
TCGTACGACTCTTAGCGG387310.39885842724492127No Hit
CGCCTGTCTGAGCGTCGCT370370.3814133270473303No Hit
CATTGCACTTGTCTCGGTCTGA319680.32921190266622713No Hit
TTCTCACTACTGCACTTGACTAG288050.29663879054994596No Hit
AACCGGGCGGAAACACCA286890.295444202815046No Hit
GGAATACCGGGTGCTGTAGGCTT280780.2891520208665642No Hit
CAACACCAGTCGATGGGCTGT280550.2889151629536099No Hit
TAGCTTATCAGACTGATGTTGACA262330.2701518969795776No Hit
TAGCAGCACGTAAATATTGGC261540.2693383415394302No Hit
TGAGGTAGTAGTTTGTGCTGTT257270.2649410228945829No Hit
CACCCGTAGAACCGACCTTGCG253250.2608011585029468No Hit
AACGGAATCCCAAAAGCAGCTG245690.25301574188584003No Hit
TGAGAACTGAATTCCATGGGTT224350.23103944683173194No Hit
CAAAGAATTCTCCTTTTGGGCT219300.22583887091686566No Hit
TTCACAGTGGCTAAGTTCCG203810.20988700538789964No Hit
CTACGCCTGTCTGAGCGTCGCT192200.19793082986877147No Hit
TGTCAGTTTGTCAAATACCCCA186800.1923698179994095No Hit
TGAATACCGGGTGCTGTAGGCTTT186800.1923698179994095No Hit
TGTCAGTTTGTCAAATACCCCAA186260.1918137168124733No Hit
AGCTCGGTCTGAGGCCCCTCAGT180100.18547004401334932No Hit
TCGTTTCCCGGCCAATGCACCA179240.18458440138230278No Hit
TTCAAGTAATCCAGGATAGGCTT174040.1792293529155098No Hit
CTTCTCACTACTGCACTTGACTAG171840.1769637554872512No Hit
AACATTCAACGCTGTCGGTGAGTT171190.17629437442890208No Hit
TACGCCTGTCTGAGCGTCGCT170490.17557350251991075No Hit
TTCAAGTAATCCAGGATAGGC167750.17275180390471598No Hit
TTCACAGTGGCTAAGTTCCGC164910.16982712358823673No Hit
CTCCCACTGCTTCACTTGACTAG163290.16815882002742816No Hit
TGTAAACATCCTACACTCAGCT163220.16808673283652903No Hit
CACCCGGCTGTGTGCACATGTGC162950.16780868224306092No Hit
TGTAAACATCCCCGACTGGAAGC161630.16644932378610577No Hit
TGAGAACTGAATTCCATAGGCTGT160320.16510026349927906No Hit
CAAAGAATTCTCCTTTTGGGCTT155520.160157141837624No Hit
TTATAAAGCAATGAGACTGATT155190.15981730222338525No Hit
CCCCCACTGCTAAATTTGACTGGCTT154920.15953925162991714No Hit
TGAGGTAGTAGGTTGTGTGGTT154700.15931269188709127No Hit
TTCACAGTGGCTAAGTTCTGC152620.1571706725003741No Hit
AATGCACCCGGGCAAGGATTCT148890.15332945504246295No Hit
CCCCCCACTGCTAAATTTGACTGG144530.1488394528664596No Hit
TGAGGTAGTAGGTTGTATAGT142120.14635759386550362No Hit
ATCACATTGCCAGGGATTTCC135890.13994183387548048No Hit
TAGCAGCACGTAAATATTGGCGT134340.13834561750557106No Hit
TATTGCACTTGTCCCGGCCTGTT132550.13650224505257885No Hit
CAACGGAATCCCAAAAGCAGCTGA131030.13493692319305475No Hit
AGCAGCATTGTACAGGGCTATGT129220.13307295439980565No Hit
TATTGCACTTGTCCCGGCCTGTAG128710.1325477477232548No Hit
AGAGGTAGTAGGTTGCATAGTT123760.127450153509673No Hit
TGAATACCGGGTGCTGTAGGCTT123270.12694554317337908No Hit
ACTGGACTTGGAGTCAGAAGGCA121980.1256170792268093No Hit
AATGACACGATCACTCCCGTTG116170.11963384238218096No Hit
CCCATAAAGTAGAAAGCACTA111480.11480400059193882No Hit
ACTGGACTTGGAGTCAGAAGGCT111410.11473191340103969No Hit
CACTAGATTGTGAGCTCCTGGA111080.1143920737868009No Hit
TGAGGTAGTAAGTTGTATTGTT110960.11426849574525952No Hit
ATCCCGGACGAGCCCCCA110830.11413461953358969No Hit
ACGCCTGTCTGAGCGTCGCT109720.11299152264933196No Hit
ACCACAGGGTAGAACCACGGAC106300.10946954846540273No Hit
ACTGGACTTGGAGTCAGAAGG104760.10788363026562173No Hit
TTCACAGTGGCTAAGTTCTG104230.107337827248814No Hit
AGCAGCATTGTACAGGGCTATG103830.10692590044367606No Hit
CACTAGATTGTGAGCTCCTGGAA102730.10579310172954678No Hit
CCCCCCACTGCTAAATTTGACTGGCT99920.10289931592345287No Hit
TGTCAGTTTGTCAAATACCCC99800.1027757378819115No Hit
CAAAGTGCTGTTCGTGCAGGT98450.10138548491457101No Hit
ACCCCCCACTGCTAAATTTGACTG97350.10025268620044174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)