FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8659371
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG89683410.356803051861387No Hit
CCCCCCACTGCTAAATTTGACTG7841269.055230454960297No Hit
TTCAAGTAATCCAGGATAGGCT3342293.859737618355883No Hit
TAGCAGCACGTAAATATTGGCG3081183.558203014976492No Hit
AAGCTGCCAGTTGAAGAACTGT2919263.371214837659687No Hit
AACATTCAACGCTGTCGGTGAGT2267672.6187467888833957No Hit
AACATTCAACGCTGTCGGTGAG2177312.5143974083106038No Hit
CCCCCACTGCTAAATTTGACTG2014742.3266585991061013No Hit
TAGCTTATCAGACTGATGTTGACT1625361.8769954538268425No Hit
TATTGCACTTGTCCCGGCCTGTAA1572171.81557066904744No Hit
TAGCTTATCAGACTGATGTTGAC1550341.7903609858037035No Hit
TATTGCACTTGTCCCGGCCTGTAT1263351.458939685111078No Hit
TAGCTTATCAGACTGATGTTGA1224471.4140403500439003No Hit
TTAATGCTAATCGTGATAGGGGTT1177501.3597985350206152No Hit
TGAGGTAGTAGGTTGTATAGTT917031.059003015346034No Hit
TGTAAACATCCTTGACTGGAAGCT885141.0221758601173228No Hit
TATTGCACTTGTCCCGGCCTGTA680230.7855420445665164No Hit
TATTGCACTTGTCCCGGCCTGT639330.7383099765560339No Hit
TTCAAGTAATTCAGGATAGGTT608330.7025106096043234No Hit
CAACGGAATCCCAAAAGCAGCTGT586280.6770468663370585No Hit
TGAGGTAGTAGATTGTATAGTT583700.6740674351520451No Hit
CAACGGAATCCCAAAAGCAGCT582550.6727393941199655No Hit
CATAAAGTAGAAAGCACTACT561640.6485921437018924No Hit
AGCAGCATTGTACAGGGCTATGA550500.635727467965052No Hit
GCCTGTCTGAGCGTCGCT539030.6224817021929191No Hit
TGTAAACATCCCCGACTGGAAGCT500170.5776054634915169No Hit
CCCATAAAGTAGAAAGCACTAC429640.49615612958493177No Hit
CAAAGAATTCTCCTTTTGGGCT427390.4935577884352108No Hit
TGTAAACATCCTACACTCTCAGCT343830.39706117222601967No Hit
CCCCCACTGCTAAATTTGACTGGCTT304540.35168836166044853No Hit
ACTGGACTTGGAGTCAGAAGGC295780.34157215345086844No Hit
TTCTCACTACTGCACTTGACTAG293780.33926251687333875No Hit
TAGCTTATCAGACTGATGTTGACA292600.3378998312925962No Hit
TGAGAACTGAATTCCATGGGTT280640.32408820455896853No Hit
CCCATAAAGTAGAAAGCACT272420.3145955982253214No Hit
CTCCCACTGCTTCACTTGACTAG251000.2898593904799783No Hit
TCGTTTCCCGGCCAATGCACCA248770.28728414569603267No Hit
TGAGGTAGTAGTTTGTACAGTT232150.2680910657367608No Hit
TGAGAACTGAATTCCATAGGCTGT225210.26007662681273275No Hit
CATTGCACTTGTCTCGGTCTGA222980.2575013820287871No Hit
AACCGGGCGGAAACACCA215870.24929062399566895No Hit
TAGCAGCACGTAAATATTGGC213520.24657680101707155No Hit
TTCACAGTGGCTAAGTTCCG213470.24651906010263333No Hit
AGCTCGGTCTGAGGCCCCTCAGT206700.23870094028769526No Hit
CAACGGAATCCCAAAAGCAGCTGA204610.2362873700641767No Hit
CTTCTCACTACTGCACTTGACTAG200760.23184131965243204No Hit
AACGGAATCCCAAAAGCAGCTG196610.2270488237540579No Hit
TATTGCACTTGTCCCGGCCTGTAG187770.21684023008137657No Hit
CACTAGATTGTGAGCTCCTGGAA179110.20683950370067294No Hit
CCCCCCACTGCTAAATTTGACTGGCT179060.20678176278623472No Hit
CACCCGTAGAACCGACCTTGCG175210.20233571237449002No Hit
ACCCCCCACTGCTAAATTTGACTG173260.20008381671139855No Hit
CCCCCCACTGCTAAATTTGACTGG170360.19673484367398048No Hit
AACATTCAACGCTGTCGGTGAGTT164820.1903371503542232No Hit
TTAATGCTAATCGTGATAGGGGTTT162590.18776190557027755No Hit
GGAATACCGGGTGCTGTAGGCTTT159630.18434364343553358No Hit
TCGTACGACTCTTAGCGG156270.18046345398528368No Hit
TGAGGTAGTAGTTTGTGCTGTT153650.17743783006871977No Hit
CAAAGAATTCTCCTTTTGGGCTT153260.1769874509361015No Hit
TATTGCACTTGTCCCGGCCTGTT152740.17638694542594374No Hit
CACCCGGCTGTGTGCACATGTGC152120.17567095808690955No Hit
CAACACCAGTCGATGGGCTGT151790.17528986805161714No Hit
CACTAGATTGTGAGCTCCTGGA150930.17429672432327936No Hit
TATTGCACTTGTCCCGGCCTGTAAA149000.1720679250259632No Hit
TTCAAGTAATCCAGGATAGGC147470.17030105304415297No Hit
TTAATGCTAATCGTGATAGGGGT139930.161593723146866No Hit
ATCACATTGCCAGGGATTTCC138470.1599076884452693No Hit
TATTGCACTTGTCCCGGCCTGTAAT138060.1594342129468757No Hit
CCCATAAAGTAGAAAGCACTA137310.15856809923030207No Hit
AATGCACCCGGGCAAGGATTCT130140.15028805209985807No Hit
TGATGTAGTAGGTTGTATAGTT129340.1493641974688462No Hit
TTCAAGTAATCCAGGATAGGCTT126810.1464425071982711No Hit
AGCTACATTGTCTGCTGGGTTT123460.14257386593090884No Hit
TGTAAACATCCCCGACTGGAAGC122260.141188083984391No Hit
TCGTTTCCCGGGCGGCGCACCA118180.13647642536623042No Hit
ATGCACCTGGGCAAGGATTCTGA117110.13524076979725203No Hit
TCCTGTCTGAGCGTCGCT116130.13410904787426245No Hit
CTACGCCTGTCTGAGCGTCGCT115150.1329773259512729No Hit
TGTCAGTTTGTCAAATACCCCA111300.12853127553952823No Hit
AGCAGCATTGTACAGGGCTATGT110750.12789612548070756No Hit
GGGAATACCGGGTGCTGTAGGCTTT109410.12634866897376265No Hit
TGAGGTAGTAGGTTGTGTGGTT107940.1246510860892783No Hit
ATCACATTGCCAGGGATTTCCA106970.1235309123491764No Hit
CTCCCACTGCTTCACTTGACTAGCCT106690.12320756322832223No Hit
TATTGCACTTGTCCCGGCCTGTTT105820.12220287131709683No Hit
TTCACAGTGGCTAAGTTCTG103850.11992787928823007No Hit
GGAATACCGGGTGCTGTAGGCTT102820.11873841645080226No Hit
CCCCCCACTGCTAAATTTGACTGTA99960.11543563614493477No Hit
TTCACAGTGGCTAAGTTCTGC96280.11118590484228011No Hit
TGTCAGTTTGTCAAATACCCCAA94940.1096384483353352No Hit
CAAAGTGCTGTTCGTGCAGGTAG91710.10590838526262474No Hit
TGTAAACATCCTACACTCAGCT87680.10125446755890237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)