FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11183976
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG140353012.54947256682239No Hit
CCCCCCACTGCTAAATTTGACTG8175327.309851165631972No Hit
AAGCTGCCAGTTGAAGAACTGT4171663.7300330401281263No Hit
TTCAAGTAATCCAGGATAGGCT3955583.536828047556612No Hit
TAGCAGCACGTAAATATTGGCG3467233.1001765382901394No Hit
AACATTCAACGCTGTCGGTGAG3240652.897583113554607No Hit
CCCCCACTGCTAAATTTGACTG2954232.6414845668481406No Hit
AACATTCAACGCTGTCGGTGAGT2768032.47499636980623No Hit
TAGCTTATCAGACTGATGTTGA1548111.3842214968987774No Hit
TAGCTTATCAGACTGATGTTGAC1452311.2985632301070746No Hit
TAGCTTATCAGACTGATGTTGACT1439161.2868053364921384No Hit
TATTGCACTTGTCCCGGCCTGTAA1424331.2735452937309595No Hit
GCCTGTCTGAGCGTCGCT1356681.2130569665027893No Hit
CATAAAGTAGAAAGCACTACT1293841.1568694353421358No Hit
TGAGGTAGTAGGTTGTATAGTT1195681.0691010066545208No Hit
TATTGCACTTGTCCCGGCCTGTAT1167881.0442440148297887No Hit
TATTGCACTTGTCCCGGCCTGT1096060.9800271388279089No Hit
TGTAAACATCCTTGACTGGAAGCT874720.7821189888104195No Hit
TCGTACGACTCTTAGCGG865610.7739734062376386No Hit
TATTGCACTTGTCCCGGCCTGTA850180.7601768816385157No Hit
CAACGGAATCCCAAAAGCAGCTGT728910.6517449608260962No Hit
TGAGGTAGTAGATTGTATAGTT681810.6096311365475033No Hit
CCCATAAAGTAGAAAGCACTAC626600.5602658660927027No Hit
CCCATAAAGTAGAAAGCACT549130.49099711945018476No Hit
AGCAGCATTGTACAGGGCTATGA539640.48251176504670606No Hit
TGTAAACATCCCCGACTGGAAGCT503780.45044803386559484No Hit
TTCAAGTAATTCAGGATAGGTT490430.438511312971344No Hit
CAACACCAGTCGATGGGCTGT479850.4290513498956006No Hit
ACTGGACTTGGAGTCAGAAGGC472290.422291678737508No Hit
CATTGCACTTGTCTCGGTCTGA402380.3597826032530828No Hit
CTCCCACTGCTTCACTTGACTAG379890.33967347569415385No Hit
AACGGAATCCCAAAAGCAGCTG370460.33124176947446954No Hit
CAACGGAATCCCAAAAGCAGCT345420.3088525941042792No Hit
CACCCGGCTGTGTGCACATGTGC337800.3020392747623922No Hit
TGAGGTAGTAGTTTGTACAGTT319950.2860789400835624No Hit
TTCACAGTGGCTAAGTTCCG318250.2845589082093882No Hit
TAGCAGCACGTAAATATTGGC300600.268777400809873No Hit
TCGTTTCCCGGCCAATGCACCA295530.2642441292792474No Hit
TGTAAACATCCTACACTCTCAGCT295470.264190481095453No Hit
TTCTCACTACTGCACTTGACTAG281940.252092815649819No Hit
CCCCCACTGCTAAATTTGACTGGCTT276760.24746118911557033No Hit
CAAAGAATTCTCCTTTTGGGCT267520.23919936881123494No Hit
CGCCTGTCTGAGCGTCGCT265640.2375183923856775No Hit
ATCCCGGACGAGCCCCCA251860.22519719284090023No Hit
GGAATACCGGGTGCTGTAGGCTTT242150.21651512843017545No Hit
TTCAAGTAATCCAGGATAGGC222840.19924935461234894No Hit
TGAGGTAGTAGTTTGTGCTGTT219990.19670106588211564No Hit
AGCTCGGTCTGAGGCCCCTCAGT215420.19261486254977658No Hit
TATTGCACTTGTCCCGGCCTGTT213220.19064776247731577No Hit
CCCATAAAGTAGAAAGCACTA212260.1897893915366056No Hit
CTTCTCACTACTGCACTTGACTAG198020.1770568892494047No Hit
TAGCTTATCAGACTGATGTTGACA193790.17327469229190048No Hit
TATTGCACTTGTCCCGGCCTGTAG193550.17306009955672294No Hit
GGAATACCGGGTGCTGTAGGCTT190990.1707711103814958No Hit
TCCTGTCTGAGCGTCGCT185620.16596959793189828No Hit
CTACGCCTGTCTGAGCGTCGCT179550.16054219000469958No Hit
AACATTCAACGCTGTCGGTGAGTT176270.15760942262393982No Hit
ATCACATTGCCAGGGATTTCC175180.15663481395167514No Hit
ACCCCCCACTGCTAAATTTGACTG174520.1560446839299369No Hit
ACTGGACTTGGAGTCAGAAGGCT174380.1559195048344167No Hit
TTCACAGTGGCTAAGTTCTG171110.15299567881762263No Hit
TTCACAGTGGCTAAGTTCCGC171060.152950971997794No Hit
CACTAGATTGTGAGCTCCTGGA169570.15161870876690006No Hit
CCCCCCACTGCTAAATTTGACTGG168610.1507603378261899No Hit
TGAGGTAGTAGGTTGTGTGGTT163070.14580682218917493No Hit
TCGTTTCCCGGGCGGCGCACCA160210.1432495920949759No Hit
AGAATTACTCTGAGACCT160110.14316017845531856No Hit
CACTAGATTGTGAGCTCCTGGAA159720.14281146526065508No Hit
TGTAAACATCCCCGACTGGAAGC155740.13925280240229415No Hit
TGTCAGTTTGTCAAATACCCCAA151380.13535436771323545No Hit
TTCACAGTGGCTAAGTTCTGC151330.1353096608934068No Hit
TGTAAACATCCCCGACTGGA149430.1336108017399179No Hit
TGTCAGTTTGTCAAATACCCCA145210.12983754614637943No Hit
ACTGGACTTGGAGTCAGAAGG140650.1257602841780061No Hit
ACTGGACTTGGAGTCAGAAGGCA140420.1255546328067943No Hit
TGATGTAGTAGGTTGTATAGTT140200.12535792279954822No Hit
CCCCCCACTGCTAAATTTGACTGGCT137880.12328352635949862No Hit
TGTAAACATCCTACACTCAGCT130140.11636291065002285No Hit
ATCCCACCGCTGCCACCA129060.1153972433417239No Hit
AATGACACGATCACTCCCGTTG128930.11528100561016941No Hit
TTATAAAGCAATGAGACTGATT127540.1140381560189328No Hit
TTCAAGTAATCCAGGATAGGCTT124820.11160610502025398No Hit
ACCACAGGGTAGAACCACGGAC122690.10970159449555329No Hit
AGCAGCATTGTACAGGGCTATGT122390.10943335357658135No Hit
AACCGGGCGGAAACACCA122160.10922770220536955No Hit
TACGCCTGTCTGAGCGTCGCT120780.10799379397809868No Hit
TATTGCACTTGTCCCGGCCTGTAAA118110.10560644979924849No Hit
CAAAGAATTCTCCTTTTGGGCTT116870.10449772066749786No Hit
TATTGCACTTGTCCCGGCCTGA115760.10350522926730171No Hit
AGCAGCATTGTACAGGGCTATG115540.10330851926005563No Hit
TGAGGTAGTAGGTTGTATAGT114130.10204778694088758No Hit
TAGCAGCACGTAAATATTGGCGT114060.10198519739312746No Hit
TGTCAGTTTGTCAAATACC112330.10043834142705599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)