FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7184030
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTT5911808.22908590303771No Hit
GGAATACCGGGTGCTGTAGGCTT4277445.954095403276434No Hit
CAACGGAATCCCAAAAGCAGCTG3063294.264027293872659No Hit
AAGCTGCCAGTTGAAGAACTGT1781552.4798755016334844No Hit
TTCAAGTAATCCAGGATAGGCT1721392.396134203225766No Hit
TAGCAGCACGTAAATATTGGCG1574592.1917920721377833No Hit
TAGCTTATCAGACTGATGTTGAC1554842.164300538834053No Hit
CCCCCCACTGCTAAATTTGACTG1487362.0703699733993317No Hit
AACATTCAACGCTGTCGGTGAGT1310351.8239762361794147No Hit
TAGCTTATCAGACTGATGTTGACT1151081.602276159759912No Hit
GGAATACCGGGTGCTGTAGGCTTA1118501.5569255696315298No Hit
GCCTGTCTGAGCGTCGCT958921.3347939805373865No Hit
TAGCTTATCAGACTGATGTTGA818631.1395136156168613No Hit
AACATTCAACGCTGTCGGTGAG765921.0661425411642211No Hit
TATTGCACTTGTCCCGGCCTGTAA759861.057707164363178No Hit
TATTGCACTTGTCCCGGCCTGTAT735101.0232418294467032No Hit
TGTAAACATCCTTGACTGGAAGCT721971.0049651797111092No Hit
GGGCTACGCCTGTCTGAGCGTCGCT705150.9815521371709194No Hit
GGAATACCGGGTGCTGTAGGCT673880.9380250360869874No Hit
TGAGGTAGTAGGTTGTATAGTT639150.8896816967635158No Hit
TGAATACCGGGTGCTGTAGGCTTT639130.8896538572361196No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT477560.6647522351660559No Hit
TATTGCACTTGTCCCGGCCTGT467240.6503870390296255No Hit
CTACGCCTGTCTGAGCGTCGCT467030.6500947239919656No Hit
AGCAGCATTGTACAGGGCTATGA460160.6405318463313767No Hit
TGAATACCGGGTGCTGTAGGCTT458250.6378731714650412No Hit
CCCCCACTGCTAAATTTGACTG451220.6280875775852829No Hit
TGAGGTAGTAGATTGTATAGTT448160.6238281298936669No Hit
AACCGGGCGGAAACACCA438760.610743552017461No Hit
TTCAAGTAATTCAGGATAGGTT367400.5114121182678802No Hit
ACTGGACTTGGAGTCAGAAGGC338320.4709334454338303No Hit
TGTAAACATCCCCGACTGGAAGCT323830.4507637078352958No Hit
TATTGCACTTGTCCCGGCCTGTA315990.4398506130959921No Hit
GGGAATACCGGGTGCTGTAGGCTTT292560.4072366067513638No Hit
CATAAAGTAGAAAGCACTACT281440.3917578295190861No Hit
TACGCCTGTCTGAGCGTCGCT267430.37225624057805995No Hit
CGCCTGTCTGAGCGTCGCT265460.36951404712953595No Hit
ATCCCGGACGAGCCCCCA262330.36515716109203333No Hit
ATACCGGGTGCTGTAGGCTTA258480.3597980520682681No Hit
TGTAAACATCCTACACTCTCAGCT239110.3328354697850649No Hit
GGGAATACCGGGTGCTGTAGGCTT237090.33002367751805045No Hit
GAATACCGGGTGCTGTAGGCTTT236460.3291467324050707No Hit
ATACCGGGTGCTGTAGGCTTT225560.313974189974151No Hit
CCCATAAAGTAGAAAGCACTAC221920.30890739598804573No Hit
CAACGGAATCCCAAAAGCAGCTGT212040.29515466945433133No Hit
GGAATACCGGGTGCTGTAGGCTA211120.2938740511941069No Hit
ATACCGGGTGCTGTAGGCTT203830.28372654345819825No Hit
TAGCTTATCAGACTGATGTTGACA194130.27022437267104954No Hit
CTCCTGGCTGGCTCGCCA185200.25779402368865384No Hit
GAATACCGGGTGCTGTAGGCTT184930.2574181900688054No Hit
TCGTACGACTCTTAGCGG174550.24296947535018648No Hit
TGAGGTAGTAGTTTGTACAGTT169160.23546672271691516No Hit
CATTGCACTTGTCTCGGTCTGA168890.23509088909706666No Hit
AATACCGGGTGCTGTAGGCTT144910.20171129574904334No Hit
CTCGACACAAGGGTTTGT144750.2014885795298739No Hit
ATCTCGGTGGAACCTCCA143170.19928925686557544No Hit
TTCAAGTAATCCAGGATAGGCTT139760.19454261744452625No Hit
CACCCGGCTGTGTGCACATGTGC133860.1863299568626523No Hit
TGAATACCGGGTGCTGTAGGCTTA133680.18607940111608665No Hit
CCCATAAAGTAGAAAGCACT132860.18493798049284316No Hit
CCCCCACTGCTAAATTTGACTGGCTT127720.17778322195202415No Hit
CAAAGAATTCTCCTTTTGGGCT127530.1775187464417604No Hit
ACGCCTGTCTGAGCGTCGCT124560.17338457662342724No Hit
ACCGGGTGCTGTAGGCTTT120970.16838738145581242No Hit
ACCGGGTGCTGTAGGCTT117960.16419753258268688No Hit
TAGCAGCACGTAAATATTGGC117710.1638495384902346No Hit
ACCGGGTGCTGTAGGCTTA115310.16050879520269262No Hit
AATACCGGGTGCTGTAGGCTTA115030.16011904181914607No Hit
GTTCGATTCCCCGACGGGGAGCCA114530.1594230536342415No Hit
TGAGGTAGTAGTTTGTGCTGTT114270.15906113977809114No Hit
AACATTCAACGCTGTCGGTGAGTT114160.15890802237741214No Hit
AATACCGGGTGCTGTAGGCTTT113460.15793363891854573No Hit
CCAAGTCCTTCTGATCGAGGCCC111210.15480169208647512No Hit
CCTCGACACAAGGGTTTGT110630.15399434579198581No Hit
AGCTCGGTCTGAGGCCCCTCAGT107680.14988801550104885No Hit
CAAAGAATTCTCCTTTTGGGCTT105480.14682566748746875No Hit
CAACGGAATCCCAAAAGCAGCT104460.1454058515902634No Hit
TGTCAGTTTGTCAAATACCCCAA104390.14530841324437677No Hit
AGCAGCATTGTACAGGGCTATGT102660.14290029412460692No Hit
TTCTCACTACTGCACTTGACTAG101000.14058961335072376No Hit
TGTAAACATCCTACACTCAGCT100980.14056177382332757No Hit
CTTCTCACTACTGCACTTGACTAG100700.140172020439781No Hit
TCCTGTCTGAGCGTCGCT100370.139712668237744No Hit
GTTCGTTTCCCGGCCAATGCACCA98890.13765254321042647No Hit
TAGCAGCACGTAAATATTGGCGT98690.13737414793646463No Hit
TGTCAGTTTGTCAAATACCCCA97820.13616312849473067No Hit
CTCCCACTGCTTCACTTGACTAG92040.1281175050772338No Hit
TGTAAACATCCCCGACTGGAAGC91440.1272823192553483No Hit
CCCCCCACTGCTAAATTTGACTGGCT91430.12726839949165022No Hit
TATTGCACTTGTCCCGGCCTGTT90550.12604346028621818No Hit
TACCGGGTGCTGTAGGCTTT90320.12572330572116208No Hit
ACTGGACTTGGAGTCAGAAGGCA89820.1250273175362575No Hit
TATTGCACTTGTCCCGGCCTGTAG89410.12445660722463575No Hit
GCGTTCGCGCTTTCCCCTG83080.11564539680374386No Hit
TTATAAAGCAATGAGACTGATT82170.11437869830721754No Hit
AGGTCCCTGTTCGGGCGCCA81880.11397502515997288No Hit
GAATACCGGGTGCTGTAGGCTTA81540.11350175319423779No Hit
CAACACCAGTCGATGGGCTGT81050.1128196847730313No Hit
ACTGGACTTGGAGTCAGAAGGCT80550.11212369658812672No Hit
AACGGAATCCCAAAAGCAGCTG79860.1111632328929584No Hit
TACCGGGTGCTGTAGGCTT78830.109729497232055No Hit
TTCCCGGCCAACGCACCA78060.10865767542730194No Hit
CACTAGATTGTGAGCTCCTGGAA76490.10647227252670159No Hit
TACCGGGTGCTGTAGGCTTA76360.1062913155986264No Hit
TTCAAGTAATCCAGGATAGGC75080.10450958584527069No Hit
ATCACATTGCCAGGGATTTCCA73140.10180915168784095No Hit
TGAATACCGGGTGCTGTAGGCT72780.10130804019470964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)