FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836566
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG63174510.823915980732506No Hit
AAGCTGCCAGTTGAAGAACTGT2418104.143018343320371No Hit
TTCAAGTAATCCAGGATAGGCT2163503.7068029385772387No Hit
CCCCCCACTGCTAAATTTGACTG2126343.6431353641850364No Hit
TAGCAGCACGTAAATATTGGCG2018523.45840345161864No Hit
AACATTCAACGCTGTCGGTGAGT1736622.9754139677337665No Hit
AACATTCAACGCTGTCGGTGAG1226502.101406888913789No Hit
GCCTGTCTGAGCGTCGCT1033371.7705102623700306No Hit
TGAGGTAGTAGGTTGTATAGTT980721.6803031097395282No Hit
CCCCCACTGCTAAATTTGACTG763851.3087318810410093No Hit
TATTGCACTTGTCCCGGCCTGTAA689021.1805229307781322No Hit
TAGCTTATCAGACTGATGTTGAC687971.178723927734219No Hit
TAGCTTATCAGACTGATGTTGA668241.1449198038709747No Hit
TATTGCACTTGTCCCGGCCTGT663011.135959055376055No Hit
TATTGCACTTGTCCCGGCCTGTAT639671.095969787714214No Hit
TAGCTTATCAGACTGATGTTGACT636151.0899388441765243No Hit
CGCCTGTCTGAGCGTCGCT523610.8971199845936806No Hit
TGAGGTAGTAGATTGTATAGTT462780.7928977415829788No Hit
TCCTGTCTGAGCGTCGCT441720.7568148805307778No Hit
CATAAAGTAGAAAGCACTACT418670.7173224803763035No Hit
CAACGGAATCCCAAAAGCAGCTGT417090.7146154091292722No Hit
TGTAAACATCCTTGACTGGAAGCT399880.6851288925714196No Hit
TCGTACGACTCTTAGCGG396510.6793549494685744No Hit
ACTGGACTTGGAGTCAGAAGGC368590.6315186018628077No Hit
AGCAGCATTGTACAGGGCTATGA356490.610787233451999No Hit
TATTGCACTTGTCCCGGCCTGTA315330.5402663141306034No Hit
TTCAAGTAATTCAGGATAGGTT303920.5207171477200806No Hit
TTAATGCTAATCGTGATAGGGGTT302230.5178216094874966No Hit
CCCATAAAGTAGAAAGCACTAC266940.4573579738496918No Hit
CAACGGAATCCCAAAAGCAGCT260290.4459642879049085No Hit
CACCCGGCTGTGTGCACATGTGC238360.4083908243306081No Hit
CCCATAAAGTAGAAAGCACT237370.40669462146063284No Hit
TGTAAACATCCCCGACTGGAAGCT207980.3563396695933876No Hit
CATTGCACTTGTCTCGGTCTGA206360.3535640648970645No Hit
AACGGAATCCCAAAAGCAGCTG203440.3485611230987536No Hit
TGAGGTAGTAGTTTGTACAGTT200790.3440207820831633No Hit
AACCGGGCGGAAACACCA181400.3107991925389005No Hit
CAACACCAGTCGATGGGCTGT178880.3064815852335089No Hit
AGCTCGGTCTGAGGCCCCTCAGT163520.2801647407054079No Hit
TGAGAACTGAATTCCATGGGTT151540.25963897264247504No Hit
TACGCCTGTCTGAGCGTCGCT150300.2575144357144252No Hit
ACCGGGTGCTGTAGGCTT148850.25503009817759276No Hit
TGAGGTAGTAGTTTGTGCTGTT135090.23145459162116902No Hit
TGAGGTAGTAGGTTGTGTGGTT133620.22893598735969062No Hit
CCCCCACTGCTAAATTTGACTGGCTT132350.22676005034467187No Hit
ATCCCGGACGAGCCCCCA131120.224652646778945No Hit
TGTAAACATCCTACACTCTCAGCT130530.22364177840188906No Hit
CTACGCCTGTCTGAGCGTCGCT129230.22141444129990134No Hit
TAGCAGCACGTAAATATTGGC127600.2186217032412552No Hit
CTCCCACTGCTTCACTTGACTAG124770.21377296170385118No Hit
TTCTCACTACTGCACTTGACTAG119950.20551468106417367No Hit
TTCAAGTAATCCAGGATAGGCTT117680.2016254078168567No Hit
ACGCCTGTCTGAGCGTCGCT115830.19845573578710496No Hit
TTCAAGTAATCCAGGATAGGC105730.18115103984089276No Hit
ACCGGGTGCTGTAGGCTTT103040.17654216537601047No Hit
CCCCCCACTGCTAAATTTGACTGGCT102520.1756512305352154No Hit
AACATTCAACGCTGTCGGTGAGTT101030.17309835954909103No Hit
TATTGCACTTGTCCCGGCCTGTT100490.17217315798365No Hit
TGAGGTAGTAGGTTGTATAGT100070.17145355676608473No Hit
TTCACAGTGGCTAAGTTCCG96430.16521701288051913No Hit
ACCGGGTGCTGTAGGCTTA94190.1613791397201711No Hit
TTCACAGTGGCTAAGTTCTGC92270.15808953415415847No Hit
AATGACACGATCACTCCCGTTG87010.14907738557226974No Hit
ACCACAGGGTAGAACCACGGAC85960.14727838252835657No Hit
CAACGGAATCCCAAAAGCAGCTGA84870.1454108460351515No Hit
TTCACAGTGGCTAAGTTCCGC84140.1441601105855738No Hit
AGCAGCATTGTACAGGGCTATGT80440.13782076652607028No Hit
ACTGGACTTGGAGTCAGAAGGCT78990.13533642898923784No Hit
CACTAGATTGTGAGCTCCTGGA77240.13233809058271592No Hit
CCCCCCACTGCTAAATTTGACTGG76290.1307104211620326No Hit
CTCCCACTGCTTCACTTGACTAGCCT75250.12892855148044244No Hit
CAAAGTGCTGTTCGTGCAGGT75100.12867155104559772No Hit
TATTGCACTTGTCCCGGCCTGTAG74930.128380283886107No Hit
CGCGACCTCAGATCAGACGTGG74500.12764354930621877No Hit
AGAGGTAGTAGGTTGCATAGTT74120.12699248153794543No Hit
CTTCTCACTACTGCACTTGACTAG74110.12697534817562245No Hit
TAGCTTATCAGACTGATGTTGACA73300.12558754582746087No Hit
GCGTTCGCGCTTTCCCCTG73280.1255532791028149No Hit
TGTAAACATCCCCGACTGGAAGC69390.11888840115917476No Hit
ATCCCACCGCTGCCACCA69030.11827160011554741No Hit
CAAAGAATTCTCCTTTTGGGCT68330.11707226475293864No Hit
TCGTTTCCCGGCCAATGCACCA66400.11376552582460303No Hit
TTATAAAGCAATGAGACTGATT65810.11275465744754708No Hit
AGCAGCATTGTACAGGGCTATG61000.10451351017019256No Hit
CAAAGTGCTGTTCGTGCAGGTAG60580.10379390895262727No Hit
TGAGAACTGAATTCCATAGGCTGT60470.10360544196707448No Hit
TTCACAGTGGCTAAGTTCTG59760.10238897324214272No Hit
ACTGGACTTGGAGTCAGAAGG58540.10029870303873886No Hit
CACTAGATTGTGAGCTCCTGGAA58380.10002456924157116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)