FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388827
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT106831914.458573735722869No Hit
CAACGGAATCCCAAAAGCAGCTG6045068.18135273704473No Hit
AAGCTGCCAGTTGAAGAACTGT2485743.364187576729026No Hit
AACATTCAACGCTGTCGGTGAGT1985212.686772880187884No Hit
TCCTGTCTGAGCGTCGCT1974602.6724133614171777No Hit
TAGCAGCACGTAAATATTGGCG1947622.6358987698588696No Hit
TTCAAGTAATCCAGGATAGGCT1893042.5620304819696007No Hit
CCCCCCACTGCTAAATTTGACTG1658262.244280452093411No Hit
AACATTCAACGCTGTCGGTGAG1291061.7473138835162876No Hit
TAGCTTATCAGACTGATGTTGA996231.348292496224367No Hit
TATTGCACTTGTCCCGGCCTGT972731.3164877185512667No Hit
TAGCTTATCAGACTGATGTTGAC919061.243851019925084No Hit
TGAGGTAGTAGGTTGTATAGTT888891.203019098972002No Hit
TCGTACGACTCTTAGCGG868251.1750850304114577No Hit
TATTGCACTTGTCCCGGCCTGTAA838981.1354711647735156No Hit
TATTGCACTTGTCCCGGCCTGTAT772551.045565148568237No Hit
TAGCTTATCAGACTGATGTTGACT727120.9840804230495585No Hit
CCCCCACTGCTAAATTTGACTG691990.9365356639152602No Hit
AACCGGGCGGAAACACCA634520.8587560650695978No Hit
CGCCTGTCTGAGCGTCGCT552500.7477506240165049No Hit
CATAAAGTAGAAAGCACTACT484950.6563288056412743No Hit
TATTGCACTTGTCCCGGCCTGTA428480.5799026015902118No Hit
TGAGGTAGTAGATTGTATAGTT413160.5591685933369397No Hit
TTAATGCTAATCGTGATAGGGGTT403320.5458511885580756No Hit
AGCAGCATTGTACAGGGCTATGA402540.5447955406182875No Hit
ACTGGACTTGGAGTCAGAAGGC397710.5382586437603696No Hit
TGTAAACATCCTTGACTGGAAGCT360480.48787175555741125No Hit
CAACGGAATCCCAAAAGCAGCTGT358010.4845288704147492No Hit
CATTGCACTTGTCTCGGTCTGA340440.46074972387362706No Hit
TTCAAGTAATTCAGGATAGGTT339230.459112116172161No Hit
CAACGGAATCCCAAAAGCAGCT305610.4136109831777087No Hit
CCCATAAAGTAGAAAGCACT304760.4124605976022987No Hit
CACCCGGCTGTGTGCACATGTGC273820.37058656265737444No Hit
CCCTGTCTGAGCGTCGCT259320.3509623381356743No Hit
CCCATAAAGTAGAAAGCACTAC232830.3151109100267201No Hit
TGTAAACATCCCCGACTGGAAGCT227920.30846574158523404No Hit
CAACACCAGTCGATGGGCTGT225240.304838643535706No Hit
ACCGGGTGCTGTAGGCTT213920.2895182144608339No Hit
AACGGAATCCCAAAAGCAGCTG191900.2597164610837417No Hit
CTACGCCTGTCTGAGCGTCGCT166660.2255567764680375No Hit
TGAGAACTGAATTCCATGGGTT164360.22244396844045747No Hit
ATCCCGGACGAGCCCCCA156750.21214463405355138No Hit
ACCGGGTGCTGTAGGCTTT154950.20970852342327137No Hit
TGAGGTAGTAGTTTGTACAGTT150110.20315809261740736No Hit
TACGCCTGTCTGAGCGTCGCT148190.20055957461177532No Hit
TGTAAACATCCTACACTCTCAGCT142870.1933595143045033No Hit
TATTGCACTTGTCCCGGCCTGTT141530.19154596527973924No Hit
AGCTCGGTCTGAGGCCCCTCAGT139820.18923166018097326No Hit
CACTAGATTGTGAGCTCCTGGA139270.18848729304394324No Hit
TGAGGTAGTAGTTTGTGCTGTT133320.18043459401607317No Hit
TTCAAGTAATCCAGGATAGGCTT130530.17665862253913917No Hit
CGCGACCTCAGATCAGACGTGG124460.1684435161359171No Hit
ACGCCTGTCTGAGCGTCGCT123970.1677803526865631No Hit
CAACGGAATCCCAAAAGCAGCTGA122220.16541191179601308No Hit
AACATTCAACGCTGTCGGTGAGTT120070.16250211298762307No Hit
TGAGGTAGTAGGTTGTGTGGTT116990.15833365702025504No Hit
ACCGGGTGCTGTAGGCTTA116770.15803591016544305No Hit
TAGCAGCACGTAAATATTGGC114670.15519378109678303No Hit
ACCACAGGGTAGAACCACGGAC111320.150659908534873No Hit
GCCTGTCTGAGCGTCGCTT109480.14816966211280896No Hit
GCGTTCGCGCTTTCCCCTG106490.14412301167695496No Hit
AGCAGCATTGTACAGGGCTATGT105260.14245833607959693No Hit
CTCGACACAAGGGTTTGT104630.14160569735899894No Hit
TTCAAGTAATCCAGGATAGGC96820.13103568401317287No Hit
CACTAGATTGTGAGCTCCTGGAA96240.13025071503230484No Hit
AATGACACGATCACTCCCGTTG94750.12823415678835084No Hit
TCGTTTCCCGGCCAATGCACCA94200.12748978965132085No Hit
ATCCCACCGCTGCCACCA93080.12597398748136882No Hit
TATTGCACTTGTCCCGGCCTGTAG91680.1240792347689288No Hit
ACCTGTCTGAGCGTCGCT91280.1235378768510888No Hit
TAGCTTATCAGACTGATGTTGACA91090.12328073184011482No Hit
TTATAAAGCAATGAGACTGATT90110.1219544049414068No Hit
CCCCCACTGCTAAATTTGACTGGCTT90030.12184613335783881No Hit
GTCCGGCCGTGTCGGCGG88950.12038446697967078No Hit
GGAATACCGGGTGCTGTAGGCTTT85850.11618894311641076No Hit
TTCACAGTGGCTAAGTTCCG84000.11368516274640075No Hit
TGAGGTAGTAGGTTGTATAGT83640.11319794062034473No Hit
AGCAGCATTGTACAGGGCTATG81640.11049115103114472No Hit
ACTGGACTTGGAGTCAGAAGG81320.11005806469687271No Hit
TTCACAGTGGCTAAGTTCTGC77900.10542945449934069No Hit
CCCATAAAGTAGAAAGCACTA77530.10492869842533868No Hit
CAAAGTGCTGTTCGTGCAGGT74990.10149107564705467No Hit
TATTGCACTTGTCCCGGCCTGA74780.10120686274018866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)