FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422478
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4712458.690583899095579No Hit
TTCAAGTAATCCAGGATAGGCT1857373.4253158795665004No Hit
CCCCCCACTGCTAAATTTGACTG1854933.420816091831078No Hit
AACATTCAACGCTGTCGGTGAGT1819503.3554769609023776No Hit
TAGCAGCACGTAAATATTGGCG1521212.8053779102469387No Hit
TCGTACGACTCTTAGCGG1494102.755382317825909No Hit
AAGCTGCCAGTTGAAGAACTGT1393162.5692312629023113No Hit
GCCTGTCTGAGCGTCGCT1392492.5679956654503715No Hit
AACATTCAACGCTGTCGGTGAG1337672.4668979754274707No Hit
TCCACCACGTTCCCGTGG757841.3975898104150908No Hit
TGAGGTAGTAGGTTGTATAGTT757101.3962251206920526No Hit
TATTGCACTTGTCCCGGCCTGT640641.1814524650906837No Hit
CCCCCACTGCTAAATTTGACTG627421.1570724676061386No Hit
TATTGCACTTGTCCCGGCCTGTAA564531.0410922829009173No Hit
TATTGCACTTGTCCCGGCCTGTAT502950.9275279678405335No Hit
CGCCTGTCTGAGCGTCGCT457360.8434520158495803No Hit
TAGCTTATCAGACTGATGTTGA444060.8189244843409231No Hit
TAGCTTATCAGACTGATGTTGAC413530.7626218123890959No Hit
ACCGGGTGCTGTAGGCTT357130.6586103253899785No Hit
TAGCTTATCAGACTGATGTTGACT340110.627222461760103No Hit
TTAATGCTAATCGTGATAGGGGTT331800.6118973650054459No Hit
CATAAAGTAGAAAGCACTACT330840.6101269567160992No Hit
TGAGGTAGTAGATTGTATAGTT316720.5840872014602917No Hit
CTACGCCTGTCTGAGCGTCGCT305560.5635062050966366No Hit
TATTGCACTTGTCCCGGCCTGTA295770.5454517288959033No Hit
CAACGGAATCCCAAAAGCAGCTGT290020.5348477209128373No Hit
ACTGGACTTGGAGTCAGAAGGC241710.44575561210206843No Hit
TCCTGTCTGAGCGTCGCT240300.44315532492709053No Hit
TTCAAGTAATTCAGGATAGGTT235740.4347458855526938No Hit
ATCCCGGACGAGCCCCCA232870.42945310243766777No Hit
AGCAGCATTGTACAGGGCTATGA228290.421006779557243No Hit
ACCGGGTGCTGTAGGCTTT218980.403837507501183No Hit
CATTGCACTTGTCTCGGTCTGA217920.4018826816816961No Hit
TGAGGTAGTAGGTTGTGTGGTT204460.3770600821248145No Hit
TGTAAACATCCTTGACTGGAAGCT193820.3574380569178888No Hit
TACGCCTGTCTGAGCGTCGCT193590.3570138965985662No Hit
ACCGGGTGCTGTAGGCTTA193370.3566081780322576No Hit
CACCCGGCTGTGTGCACATGTGC193230.3563499934900612No Hit
TGAGAACTGAATTCCATGGGTT191630.3533993130078167No Hit
CCCATAAAGTAGAAAGCACT181360.3344596326624101No Hit
CCCATAAAGTAGAAAGCACTAC178330.3288717814991596No Hit
ATCCCACCGCTGCCACCA163500.3015226617793562No Hit
TGAGGTAGTAGTTTGTACAGTT163270.3010985014600336No Hit
CAACACCAGTCGATGGGCTGT157430.29032851769984125No Hit
AGCTCGGTCTGAGGCCCCTCAGT157270.29003344965161687No Hit
AACCGGGCGGAAACACCA150880.27824916947565304No Hit
TCAGGCTCAGTCCCCTCCCGA148570.27398912452941254No Hit
CTCCCACTGCTTCACTTGACTAG146770.2706696089868876No Hit
AACGGAATCCCAAAAGCAGCTG145220.26781113726971323No Hit
CCCCCACTGCTAAATTTGACTGGCTT142770.2632929077812764No Hit
TCAGGCTCAGTCCCCTCCCGAT135030.24901899094841878No Hit
CGCGACCTCAGATCAGACGTGG134470.2479862527796332No Hit
ACGCCTGTCTGAGCGTCGCT128950.23780640511588982No Hit
TGTAAACATCCCCGACTGGAAGCT128690.2373269195375251No Hit
CCCCCCACTGCTAAATTTGACTGGCT118800.2190880258066515No Hit
TTCACAGTGGCTAAGTTCCG116040.2139981019747798No Hit
TGTAAACATCCTACACTCTCAGCT115460.2129284802999662No Hit
TGAGGTAGTAGTTTGTGCTGTT112550.20756193017288405No Hit
TTCAAGTAATCCAGGATAGGC111080.20485099247982194No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109720.20234291406991417No Hit
TCGTTTCCCGGCCAATGCACCA102010.18812432249609864No Hit
TCGTTTCCCGGGCGGCGCACCA100200.1847863652005596No Hit
AACATTCAACGCTGTCGGTGAGTT98760.1821307527665396No Hit
TATTGCACTTGTCCCGGCCTGTT95740.17656134335630314No Hit
TAGCAGCACATCATGGTTTACA93550.17252259944623105No Hit
CTCGTACGACTCTTAGCGG93020.17154518653648756No Hit
TGAGGTAGTAGGTTGTATAGT90830.16750644262641545No Hit
AATGACACGATCACTCCCGTTG89250.16459264565019904No Hit
TTCACAGTGGCTAAGTTCCGC88970.16407627656580626No Hit
CCCCCCACTGCTAAATTTGACTGG84870.15651515783005482No Hit
CACTAGATTGTGAGCTCCTGGA81890.1510195154318745No Hit
TAGCAGCACGTAAATATTGGC81340.15000521901610298No Hit
CAACGGAATCCCAAAAGCAGCT79970.14747869885318113No Hit
TTCTCACTACTGCACTTGACTAG78460.14469399414806294No Hit
GGAATACCGGGTGCTGTAGGCTT77200.14237033326829543No Hit
TGAGAACTGAATTCCATAGGCTGT76700.14144824561759403No Hit
GGGCTACGCCTGTCTGAGCGTCGCT75180.13864509915946177No Hit
GGAATACCGGGTGCTGTAGGCTTT74860.13805496306301288No Hit
TATTGCACTTGTCCCGGCCTGTAG74760.13787054553287262No Hit
CGCGACCTCAGATCAGACGTGGCG74690.13774145326177442No Hit
AGAGGTAGTAGGTTGCATAGTT74240.13691157437614315No Hit
TCCCCGGCACCTCCACCA72150.13305724799621133No Hit
GCGTTCGCGCTTTCCCCTG70380.12979305771272837No Hit
TTCACCACCTTCTCCACCCAGC67960.12533015348333362No Hit
GTCCGGCCGTGTCGGCGG67950.1253117117303196No Hit
AACATTCAACGCTGTCGGTGA66230.1221397302119068No Hit
ACCACAGGGTAGAACCACGGAC65450.12070127347681263No Hit
CTCCCACTGCTTCACTTGACTAGCCT63620.11732643267524552No Hit
TTCAAGTAATCCAGGATAGGCTT62040.11441263569902911No Hit
CTCGACACAAGGGTTTGT61810.11398847537970648No Hit
ACTGGACTTGGAGTCAGAAGGCT61750.11387782486162232No Hit
TGTAAACATCCTACACTCAGCT60660.11186767378309327No Hit
CAAAGTGCTGTTCGTGCAGGT59830.11033700828292894No Hit
TGAGGTAGTAGGTTGTGTGGTTT59330.10941492063222755No Hit
TCGTTTCCCGGCCAACGCACCA59260.10928582836112936No Hit
AACATTCAACGCTGTCGGTGAGA56980.105081108673931No Hit
AGCAGCATTGTACAGGGCTATGT56070.10340290914965446No Hit
ATCACATTGCCAGGGATTTCC55800.1029049818182757No Hit
CACTAGATTGTGAGCTCCTGGAA55050.10152185034222362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)