FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7889541
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG93152511.807087383157018No Hit
CCCCCCACTGCTAAATTTGACTG3074623.897083493196879No Hit
TTCAAGTAATCCAGGATAGGCT3063673.8832043587833565No Hit
TAGCAGCACGTAAATATTGGCG2768853.509519755331774No Hit
AAGCTGCCAGTTGAAGAACTGT2312312.9308549128523445No Hit
AACATTCAACGCTGTCGGTGAGT2244092.8443860042048073No Hit
AACATTCAACGCTGTCGGTGAG1962552.4875338121698083No Hit
TATTGCACTTGTCCCGGCCTGTAA1586592.0110041889635912No Hit
TATTGCACTTGTCCCGGCCTGTAT1386721.7576687921388583No Hit
TAGCTTATCAGACTGATGTTGACT1057311.3401413339508597No Hit
TGAGGTAGTAGGTTGTATAGTT1045421.3250707487292352No Hit
CCCCCACTGCTAAATTTGACTG965201.2233918297655086No Hit
TAGCTTATCAGACTGATGTTGAC952011.206673493426297No Hit
TAGCTTATCAGACTGATGTTGA875201.1093167523940872No Hit
GCCTGTCTGAGCGTCGCT831361.0537495147056084No Hit
TATTGCACTTGTCCCGGCCTGT762190.9660764802413728No Hit
TGTAAACATCCTTGACTGGAAGCT642510.8143819773545762No Hit
TATTGCACTTGTCCCGGCCTGTA599910.7603864407321035No Hit
CAACGGAATCCCAAAAGCAGCTGT581600.7371784999913176No Hit
TGAGGTAGTAGATTGTATAGTT574150.7277356185866832No Hit
CATAAAGTAGAAAGCACTACT573840.7273426933201818No Hit
CCCCCACTGCTAAATTTGACTGGCTT495950.6286170513595151No Hit
AGCAGCATTGTACAGGGCTATGA484380.613952066412989No Hit
CCCATAAAGTAGAAAGCACTAC466580.5913905511106413No Hit
ACTGGACTTGGAGTCAGAAGGC454200.5756988904677725No Hit
TGTAAACATCCCCGACTGGAAGCT407060.5159488999423414No Hit
TTCAAGTAATTCAGGATAGGTT393740.49906578849137107No Hit
TCGTACGACTCTTAGCGG374080.4741467215900139No Hit
CACCCGGCTGTGTGCACATGTGC325550.4126349048696242No Hit
CCCCCCACTGCTAAATTTGACTGGCT268850.34076760612562884No Hit
CCCATAAAGTAGAAAGCACT254490.32256629378058876No Hit
TGAGGTAGTAGTTTGTACAGTT254120.3220973184625062No Hit
CTCCCACTGCTTCACTTGACTAG243420.30853505926390395No Hit
CATTGCACTTGTCTCGGTCTGA235850.2989400777561077No Hit
AACGGAATCCCAAAAGCAGCTG227400.288229695491791No Hit
CAACGGAATCCCAAAAGCAGCT219790.27858401394960747No Hit
TTCTCACTACTGCACTTGACTAG212300.26909043251058584No Hit
TAGCAGCACGTAAATATTGGC207540.2630571284184974No Hit
TGTAAACATCCTACACTCTCAGCT205510.26048410167334196No Hit
CAAAGAATTCTCCTTTTGGGCT189770.24053363814194006No Hit
CGCCTGTCTGAGCGTCGCT188210.23855633680083546No Hit
TATTGCACTTGTCCCGGCCTGTAG182580.2314203069608232No Hit
AGCTCGGTCTGAGGCCCCTCAGT181320.2298232558776233No Hit
CTACGCCTGTCTGAGCGTCGCT178730.22654042865104573No Hit
TTCAAGTAATCCAGGATAGGC167890.21280071933208788No Hit
ACTGGACTTGGAGTCAGAAGGCT162860.20642519000788512No Hit
GTCAGGATGGCCGAGCGGTCTAAGG161630.20486616395047572No Hit
CTTCTCACTACTGCACTTGACTAG160900.2039408883229075No Hit
CAACACCAGTCGATGGGCTGT159460.20211568708496477No Hit
TATTGCACTTGTCCCGGCCTGTT157990.20025246082123155No Hit
CACTAGATTGTGAGCTCCTGGAA153220.19420648172054622No Hit
AACATTCAACGCTGTCGGTGAGTT150020.19015047896956236No Hit
TTCAAGTAATCCAGGATAGGCTT149820.18989697879762585No Hit
CACTAGATTGTGAGCTCCTGGA143920.1824187237254994No Hit
ACTGGACTTGGAGTCAGAAGGCA142900.18112587284862325No Hit
TAGCTTATCAGACTGATGTTGACA142460.18056817247036297No Hit
GGAATACCGGGTGCTGTAGGCTTT142380.18046677240158837No Hit
CTCCCACTGCTTCACTTGACTAGCCT139340.1766135697881537No Hit
TGAGGTAGTAGTTTGTGCTGTT137470.17424334318054752No Hit
TGTAAACATCCCCGACTGGAAGC134150.17003524032640174No Hit
TCGTTTCCCGGCCAATGCACCA133500.16921136476760815No Hit
TTCACAGTGGCTAAGTTCCG130660.16561166232610997No Hit
TGATGTAGTAGGTTGTATAGTT129110.16364703599360217No Hit
TATTGCACTTGTCCCGGCCTGTAAA124680.15803200718520888No Hit
TTAATGCTAATCGTGATAGGGGTT121980.15460975486406625No Hit
CCCCCCACTGCTAAATTTGACTGG121140.15354505414193298No Hit
AGCAGCATTGTACAGGGCTATGT120000.15210010316189498No Hit
TGAGGTAGTAGGTTGTGTGGTT119710.15173252791258707No Hit
TATTGCACTTGTCCCGGCCTGTAAT116550.1477272251959905No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC114330.14491337328749543No Hit
TATTGCACTTGTCCCGGCCTGTTT113370.14369657246220027No Hit
GGAATACCGGGTGCTGTAGGCTT113120.14337969724727964No Hit
CAAAGAATTCTCCTTTTGGGCTT112330.14237837156813052No Hit
ACTGGACTTGGAGTCAGAAGG110410.13994476991754018No Hit
CCCATAAAGTAGAAAGCACTA110040.1394757945994577No Hit
TTCACAGTGGCTAAGTTCCGC107520.13628169243305788No Hit
ATCACATTGCCAGGGATTTCC106990.1356099169774262No Hit
TCGTTTCCCGGGCGGCGCACCA106790.1353564168054897No Hit
TAGCAGCACGTAAATATTGGCGT102730.13021036331517893No Hit
ACCCCCCACTGCTAAATTTGACTG102270.12962731291972499No Hit
TTCACAGTGGCTAAGTTCTGC102150.1294752128165631No Hit
CCCCCACTGCTAAATTTGACTGGCT99720.12639518572753472No Hit
TACGCCTGTCTGAGCGTCGCT97260.12327713361271588No Hit
TGAGGTAGTAGGTTGTATAGT96020.12170543254670961No Hit
ATGCACCTGGGCAAGGATTCTGA92440.11716777946904644No Hit
ATCCCGGACGAGCCCCCA92050.11667345413377027No Hit
TGTCAGTTTGTCAAATACCCCAA91290.1157101534804116No Hit
AACCGGGCGGAAACACCA91190.11558340339444335No Hit
AGAGGTAGTAGGTTGCATAGTT87790.111273900471523No Hit
TGAGAACTGAATTCCATGGGTT86780.1099937246032437No Hit
CCCCCACTGCTAAATTTGACTGGCTA83280.1055574715943551No Hit
TGTCAGTTTGTCAAATACCCCA82640.10474627104415833No Hit
TATTGCACTTGTCCCGGCCTGA82530.10460684594959327No Hit
ACCACAGGGTAGAACCACGGAC82240.10423927070028535No Hit
AACATTCAACGCTGTCGGTGAGA82040.10398577052834887No Hit
ATCACATTGCCAGGGATTTCCA80900.10254081954831086No Hit
TTCACAGTGGCTAAGTTCTG79290.10050014316422211No Hit
ATCCCACCGCTGCCACCA78920.10003116784613958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)