FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12752360
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9363387.342468374481273No Hit
CCCCCCACTGCTAAATTTGACTG9078357.118956804858081No Hit
AAGCTGCCAGTTGAAGAACTGT4088953.206426104658275No Hit
AACATTCAACGCTGTCGGTGAGT4022973.154686661919833No Hit
TTCAAGTAATCCAGGATAGGCT3998253.1353020146859087No Hit
TAGCAGCACGTAAATATTGGCG3417302.679739279631378No Hit
CCCCCACTGCTAAATTTGACTG2732672.1428739464695163No Hit
AACATTCAACGCTGTCGGTGAG2637902.0685582903870343No Hit
TAGCTTATCAGACTGATGTTGA2231971.750240739753269No Hit
TGAGGTAGTAGGTTGTATAGTT2181391.7105774931071582No Hit
TAGCTTATCAGACTGATGTTGAC2168761.7006734439742919No Hit
TAGCTTATCAGACTGATGTTGACT2042491.6016564777029507No Hit
TATTGCACTTGTCCCGGCCTGTAT1923161.5080816413589329No Hit
TATTGCACTTGTCCCGGCCTGTAA1853441.4534094081409246No Hit
TGAGGTAGTAGATTGTATAGTT1817741.4254145899268842No Hit
TATTGCACTTGTCCCGGCCTGT1625851.2749404816049736No Hit
CATAAAGTAGAAAGCACTACT1430141.121470849317303No Hit
TTCAAGTAATTCAGGATAGGTT1164720.913336825497398No Hit
AGCAGCATTGTACAGGGCTATGA965640.7572245451038083No Hit
TGTAAACATCCTTGACTGGAAGCT910960.7143462072902584No Hit
TATTGCACTTGTCCCGGCCTGTA868730.6812307682656387No Hit
TCGTACGACTCTTAGCGG824110.6462411663409753No Hit
ACTGGACTTGGAGTCAGAAGGC729690.5721999692605917No Hit
GCCTGTCTGAGCGTCGCT709720.5565401227694324No Hit
CCCATAAAGTAGAAAGCACTAC647820.5080000878268807No Hit
TGAGGTAGTAGTTTGTACAGTT636550.49916250795931105No Hit
ATCCCGGACGAGCCCCCA615660.48278122637692167No Hit
CGCCTGTCTGAGCGTCGCT602890.47276739364321585No Hit
CATTGCACTTGTCTCGGTCTGA553880.4343352916636607No Hit
TGTAAACATCCCCGACTGGAAGCT548840.4303830820334432No Hit
CAACGGAATCCCAAAAGCAGCTGT547920.42966164694221304No Hit
CCCATAAAGTAGAAAGCACT541720.4247998017621836No Hit
CAACACCAGTCGATGGGCTGT534110.4188322788879862No Hit
TTCTCACTACTGCACTTGACTAG494610.3878576200797343No Hit
CTCCCACTGCTTCACTTGACTAG438400.3437795043427256No Hit
TGAGGTAGTAGTTTGTGCTGTT395410.3100680971992635No Hit
CTTCTCACTACTGCACTTGACTAG373300.29273012995241665No Hit
CAACGGAATCCCAAAAGCAGCT353520.277219275490968No Hit
TGTAAACATCCTACACTCTCAGCT287250.22525242386507283No Hit
TGAGGTAGTAGGTTGTATAGT286280.22449178034497141No Hit
CACTAGATTGTGAGCTCCTGGA274410.21518369933094736No Hit
TTATAAAGCAATGAGACTGATT270350.21199997490660552No Hit
AACCGGGCGGAAACACCA269150.2110589726136966No Hit
AACATTCAACGCTGTCGGTGAGTT264810.207655680987676No Hit
TATTGCACTTGTCCCGGCCTGTT259480.20347606247000555No Hit
AGCTCGGTCTGAGGCCCCTCAGT246390.19321129579152407No Hit
TTCACAGTGGCTAAGTTCCG246230.19308582881913622No Hit
CAAAGAATTCTCCTTTTGGGCT245230.19230166024171214No Hit
TTCACAGTGGCTAAGTTCCGC240480.18857685949894765No Hit
TGTCAGTTTGTCAAATACCCCAA238800.18725945628887516No Hit
CGCGACCTCAGATCAGACGTGGCG238100.1867105382846783No Hit
ACCGGGTGCTGTAGGCTT237860.18652233782609648No Hit
TTCAAGTAATCCAGGATAGGCTT231110.18122919992848383No Hit
TAGCTTATCAGACTGATGTTGACA227750.17859439350833883No Hit
TGAGGTAGTAGATTGTATAGT227500.17839835136398283No Hit
CTACGCCTGTCTGAGCGTCGCT219860.1724073034324627No Hit
AACGGAATCCCAAAAGCAGCTG218150.17106637516506748No Hit
TATTGCACTTGTCCCGGCCTGTAG218010.1709565915642281No Hit
ACCGGGTGCTGTAGGCTTA213160.1671533739637212No Hit
TCCTGTCTGAGCGTCGCT212580.16669855618881524No Hit
TTCAAGTAATCCAGGATAGGC212080.1663064719001032No Hit
CACCCGGCTGTGTGCACATGTGC210090.16474597643102923No Hit
CACTAGATTGTGAGCTCCTGGAA209930.16462050945864137No Hit
ACTGGACTTGGAGTCAGAAGGCA207300.162558146100016No Hit
CCCCCCACTGCTAAATTTGACTGG205770.16135836817655713No Hit
TGAGAACTGAATTCCATGGGTT204870.16065261645687542No Hit
ACTGGACTTGGAGTCAGAAGG202110.1584883111831849No Hit
CAAAGAATTCTCCTTTTGGGCTT198570.1557123544191036No Hit
TACGCCTGTCTGAGCGTCGCT197680.15501444438519615No Hit
AGCAGCATTGTACAGGGCTATGT189390.14851368687835037No Hit
ACCCCCCACTGCTAAATTTGACTG188460.14778441010134594No Hit
TTCACAGTGGCTAAGTTCTGC187690.14718060029672939No Hit
TGAGGTAGTAGGTTGTGTGGTT186580.14631017317578862No Hit
TAGCAGCACGTAAATATTGGC184550.14471831096361773No Hit
TTCACAGTGGCTAAGTTCTG182840.1433773826962225No Hit
TGTCAGTTTGTCAAATACCCCA179800.14099351022085324No Hit
TGATGTAGTAGGTTGTATAGTT175960.13798230288354468No Hit
TATTGCACTTGTCCCGGCCTGTTT171470.13446138597091048No Hit
CAAAGTGCTGTTCGTGCAGGTAG169530.13294009893070774No Hit
CCCCCACTGCTAAATTTGACTGGCTT168720.13230492238299418No Hit
ACCACAGGGTAGAACCACGGAC166220.13034450093943395No Hit
TGTAAACATCCCCGACTGGAAGC163870.12850170478248732No Hit
TATTGCACTTGTCCCGGCCTGA162520.1274430772029648No Hit
GACCCGCGGGCGCTCTCCAGTCCT161150.12636876625189375No Hit
TCGTTTCCCGGGCGGCGCACCA160150.12558459767446967No Hit
ATCCCACCGCTGCCACCA159810.12531798035814548No Hit
ACGCCTGTCTGAGCGTCGCT159780.12529445530082275No Hit
AGCAGCATTGTACAGGGCTATG155440.12189116367480216No Hit
TACCACCCTGAACGCGCCCGA153160.12010325931827522No Hit
CCCATAAAGTAGAAAGCACTA151720.11897405656678449No Hit
ACCGGGTGCTGTAGGCTTT151610.11888779802326785No Hit
CAAAGTGCTGTTCGTGCAGGT151590.11887211465171937No Hit
CCCCCCACTGCTAAATTTGACTGGCT150660.11814283787471495No Hit
AACATTCAACGCTGTCGGTGA149900.11754686975587263No Hit
TCGTTTCCCGGCCAATGCACCA144670.1134456680959446No Hit
TGATGTAGTAGATTGTATAGTT144550.11335156786665371No Hit
TGAGGTAGTAAGTTGTATTGTT141460.11092848696241324No Hit
GGAATACCGGGTGCTGTAGGCTTT139800.10962676712388923No Hit
ACTGGACTTGGAGTCAGAAGGCT133560.10473355520076283No Hit
GGAATACCGGGTGCTGTAGGCTT132070.10356514402040094No Hit
ACTGGACTTGGAGTCAGAAGGA131830.10337694356181915No Hit
AATGACACGATCACTCCCGTTG130440.10228694923919966No Hit
CGCGACCTCAGATCAGACGTGG130020.10195759843668152No Hit
TATTGCACTTGTCCCGGCCTGTAAT129330.1014165221182589No Hit
TAGCTTATCAGACTGATGTTG129010.10116558817348319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)