FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752686
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6212939.20067955181094No Hit
CAACGGAATCCCAAAAGCAGCTG3831985.674749277546742No Hit
AAGCTGCCAGTTGAAGAACTGT2232743.3064472418827116No Hit
AACATTCAACGCTGTCGGTGAGT2134733.161304997744601No Hit
TAGCTTATCAGACTGATGTTGAC1964022.908501890951245No Hit
TAGCAGCACGTAAATATTGGCG1847682.7362148928589307No Hit
TTCAAGTAATCCAGGATAGGCT1708882.530667056042588No Hit
CCCCCACTGCTAAATTTGACTG1680352.4884172016883355No Hit
TAGCTTATCAGACTGATGTTGACT1605262.377217006684451No Hit
AACATTCAACGCTGTCGGTGAG1250701.8521518696412065No Hit
TATTGCACTTGTCCCGGCCTGTAA1074771.5916185055842964No Hit
TATTGCACTTGTCCCGGCCTGTAT960841.422900457684542No Hit
TAGCTTATCAGACTGATGTTGA950161.407084529030374No Hit
TGTAAACATCCTTGACTGGAAGCT886311.3125295623104642No Hit
TGAGGTAGTAGGTTGTATAGTT666740.9873700628164851No Hit
CGCCTGTCTGAGCGTCGCT656660.9724426694799668No Hit
AGCAGCATTGTACAGGGCTATGA648530.9604030159258108No Hit
GCCTGTCTGAGCGTCGCT638990.9462753043751776No Hit
TATTGCACTTGTCCCGGCCTGT583240.8637155644435414No Hit
TTAATGCTAATCGTGATAGGGGTT489200.7244524623238812No Hit
TGTAAACATCCCCGACTGGAAGCT453920.6722065856460674No Hit
TATTGCACTTGTCCCGGCCTGTA448470.6641357231774141No Hit
TGAGGTAGTAGATTGTATAGTT445270.659396868149948No Hit
ACTGGACTTGGAGTCAGAAGGC431540.6390642182977263No Hit
CATAAAGTAGAAAGCACTACT370260.5483151445217503No Hit
TTCAAGTAATTCAGGATAGGTT318740.472019578579546No Hit
TGTAAACATCCTACACTCTCAGCT269590.3992337271420588No Hit
TGTCAGTTTGTCAAATACCCCAA265450.39310283345027447No Hit
TAGCTTATCAGACTGATGTTGACA246700.36533610477371525No Hit
TACGCCTGTCTGAGCGTCGCT245670.3638107858117496No Hit
GCGTTCGCGCTTTCCCCTG238800.35363705642465826No Hit
CAACGGAATCCCAAAAGCAGCTGT232570.3444110980430602No Hit
CCCATAAAGTAGAAAGCACTAC229380.33968705193755494No Hit
AACATTCAACGCTGTCGGTGAGTT200920.29754086003702823No Hit
CTACGCCTGTCTGAGCGTCGCT191390.28342795740835575No Hit
CATTGCACTTGTCTCGGTCTGA188260.27879276483461546No Hit
TGAGGTAGTAGTTTGTACAGTT185850.27522381464205503No Hit
CAACACCAGTCGATGGGCTGT169400.2508631380164871No Hit
TGTCAGTTTGTCAAATACCCCA161010.23843845249134935No Hit
CCCATAAAGTAGAAAGCACT159480.23617268743134212No Hit
ACTGGACTTGGAGTCAGAAGGCA158550.23479545768898483No Hit
CAAAGAATTCTCCTTTTGGGCT153920.22793892682111977No Hit
TTCTCACTACTGCACTTGACTAG141250.20917602269674615No Hit
CTTCTCACTACTGCACTTGACTAG140610.20822825169125292No Hit
GGAATACCGGGTGCTGTAGGCTTT137210.20319321822457018No Hit
TGAGGTAGTAGTTTGTGCTGTT137130.20307474684888352No Hit
CAAAGAATTCTCCTTTTGGGCTT136840.20264528811201943No Hit
ACGCCTGTCTGAGCGTCGCT136820.20261567026809776No Hit
TTATAAAGCAATGAGACTGATT134800.19962426803200978No Hit
TTCAAGTAATCCAGGATAGGCTT133960.19838031858729993No Hit
CAACGGAATCCCAAAAGCAGCT132710.19652920334219595No Hit
AGCAGCATTGTACAGGGCTATGT130720.1935822278719905No Hit
CCCCCACTGCTAAATTTGACTGGCTT129930.1924123230370848No Hit
TCGTACGACTCTTAGCGG128030.1895986278645268No Hit
CGCGACCTCAGATCAGACGTGGCG126890.187910410760992No Hit
TATTGCACTTGTCCCGGCCTGTAG123780.18330483603117337No Hit
CCCCCCACTGCTAAATTTGACTGG120370.1782549936425298No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT119780.17738126724684075No Hit
TAGCAGCACGTAAATATTGGC116570.1726276032974138No Hit
ACCCCCCACTGCTAAATTTGACTG114690.16984352596877747No Hit
TGTAAACATCCCCGACTGGAAGC110390.1634756895256199No Hit
GGAATACCGGGTGCTGTAGGCTT106400.1575669296632481No Hit
TATTGCACTTGTCCCGGCCTGTT105670.15648587836010738No Hit
CACTAGATTGTGAGCTCCTGGAA104940.15540482705696668No Hit
TTCACAGTGGCTAAGTTCCG104280.15442743820755178No Hit
TTAATGCTAATCGTGATAGGGGTTT104150.15423492222206098No Hit
TGTAAACATCCTACACTCAGCT103320.15300578169931195No Hit
CCAAGTCCTTCTGATCGAGGCCC102850.15230976236715288No Hit
TAGCAGCACGTAAATATTGGCGT99870.14789670362282506No Hit
ACTGGACTTGGAGTCAGAAGG98980.14657870956831104No Hit
GGGCTACGCCTGTCTGAGCGTCGCT97280.14406119283496965No Hit
ACTGGACTTGGAGTCAGAAGGCT96670.14315784859535893No Hit
CACTAGATTGTGAGCTCCTGGA96490.14289128800006398No Hit
AACGGAATCCCAAAAGCAGCTG95340.14118826197456835No Hit
CTCCCACTGCTTCACTTGACTAG93320.13819685973848037No Hit
TATTGCACTTGTCCCGGCCTGTAAT92970.13767854746985125No Hit
ATCACATTGCCAGGGATTTCCA90390.1338578456039567No Hit
TGAGAACTGAATTCCATAGGCTGT90330.1337689920721917No Hit
AGCAGCATTGTACAGGGCTATG88490.13104415043139872No Hit
ACCACAGGGTAGAACCACGGAC84470.1250909638031444No Hit
CCTCGACACAAGGGTTTGT84140.12460226937843696No Hit
AACCGGGCGGAAACACCA83720.12398029465608203No Hit
CACCCGTAGAACCGACCTTGCG81460.12063347829293411No Hit
ACCGGGTGCTGTAGGCTT81440.12060386044901245No Hit
CAAGTCCTTCTGATCGAGGCCC81430.1205890515270516No Hit
TCGTTTCCCGGCCAATGCACCA80710.11952280914587174No Hit
ATCCCGGACGAGCCCCCA80260.11885640765763432No Hit
TATTGCACTTGTCCCGGCCTGTAAA79150.117212617319982No Hit
CTGCGTTCGCGCTTTCCCCTG79150.117212617319982No Hit
CAAAGTGCTGTTCGTGCAGGTAG79080.11710895486625619No Hit
TTCACAGTGGCTAAGTTCCGC76950.11395465448859904No Hit
TGAGAACTGAATTCCATGGGTT75870.11235529091682923No Hit
CCCCCCACTGCTAAATTTGACTGGCT75170.11131866637957104No Hit
TGAGGTAGTAGGTTGTGTGGTT74010.10960083143211456No Hit
TTCAAGTAATCCAGGATAGGC73250.10847535336309137No Hit
ATAGGCGATAGAAATTGAAACCT73240.10846054444113053No Hit
TGTAAACATCCTTGACTGGAAGC71050.10521739053170841No Hit
TATTGCACTTGTCCCGGCCTGTTT71000.10514334592190426No Hit
ATGCACCTGGGCAAGGATTCTGA70680.10466946041915764No Hit
CCCCCCACTGCTAAATTTGACTGTA69500.10292200762777952No Hit
GACCCGCGGGCGCTCTCCAGTCCT68730.1017817206367955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)