FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8456633
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6295927.444948834837694No Hit
CAACGGAATCCCAAAAGCAGCTG3802524.496494053839158No Hit
AAGCTGCCAGTTGAAGAACTGT2595563.0692593612611545No Hit
AACATTCAACGCTGTCGGTGAGT2584473.0561453949816673No Hit
CGCCTGTCTGAGCGTCGCT2178612.5762144342789854No Hit
TTCAAGTAATCCAGGATAGGCT2108522.4933327483881587No Hit
TAGCAGCACGTAAATATTGGCG2002962.3685076554699727No Hit
CCCCCACTGCTAAATTTGACTG1957152.3143371599547953No Hit
AACATTCAACGCTGTCGGTGAG1518231.7953126261953192No Hit
TAGCTTATCAGACTGATGTTGAC1421891.6813902175960576No Hit
TAGCTTATCAGACTGATGTTGA991531.1724879156988366No Hit
TAGCTTATCAGACTGATGTTGACT965461.1416600436604025No Hit
TATTGCACTTGTCCCGGCCTGT917901.0854201666313295No Hit
TGAGGTAGTAGGTTGTATAGTT821000.9708355559476212No Hit
TATTGCACTTGTCCCGGCCTGTAA721780.8535075366283484No Hit
TGTAAACATCCTTGACTGGAAGCT682290.80681046463764No Hit
TATTGCACTTGTCCCGGCCTGTAT673090.79593143039316No Hit
CATAAAGTAGAAAGCACTACT606360.7170229570090129No Hit
TACGCCTGTCTGAGCGTCGCT592270.7003614795628473No Hit
GCCTGTCTGAGCGTCGCT583590.6900973472539249No Hit
AGCAGCATTGTACAGGGCTATGA573890.6786270611483317No Hit
TATTGCACTTGTCCCGGCCTGTA554730.6559702898304798No Hit
TTAATGCTAATCGTGATAGGGGTT550290.6507199733037959No Hit
TGAGGTAGTAGATTGTATAGTT496240.5868056471174757No Hit
GCGTTCGCGCTTTCCCCTG490510.5800299007891202No Hit
ACCGGGTGCTGTAGGCTT475580.5623751202162847No Hit
ACGCCTGTCTGAGCGTCGCT422680.4998206733105244No Hit
CTACGCCTGTCTGAGCGTCGCT410010.48483835114991986No Hit
ACTGGACTTGGAGTCAGAAGGC389700.46082170055150795No Hit
TCGTACGACTCTTAGCGG389040.4600412480948387No Hit
TTCAAGTAATTCAGGATAGGTT370560.43818857930810046No Hit
TGTAAACATCCCCGACTGGAAGCT350570.41455032990080093No Hit
ATCCCGGACGAGCCCCCA338630.40043123545742143No Hit
CAACACCAGTCGATGGGCTGT331540.3920472840668384No Hit
TTCACAGTGGCTAAGTTCCG312200.36917766207898584No Hit
CATTGCACTTGTCTCGGTCTGA272640.3223978148277216No Hit
AACATTCAACGCTGTCGGTGAGTT272630.32238598979049937No Hit
ACCGGGTGCTGTAGGCTTT260340.3078530190443407No Hit
CCCATAAAGTAGAAAGCACT250070.29570870581707875No Hit
TCCTGTCTGAGCGTCGCT229790.2717275303303336No Hit
CCCATAAAGTAGAAAGCACTAC225720.2669147401808734No Hit
TTCTCACTACTGCACTTGACTAG222860.2635327795353068No Hit
TGAGGTAGTAGTTTGTACAGTT221990.2625040012969701No Hit
TCGTTCGCGCTTTCCCCTG216760.2563195068297276No Hit
ACCGGGTGCTGTAGGCTTA215870.2552670785169464No Hit
CAACGGAATCCCAAAAGCAGCTGT209010.2471551029824754No Hit
TGTAAACATCCTACACTCTCAGCT205250.2427088889869053No Hit
AACCGGGCGGAAACACCA202900.23993000523967398No Hit
TGTCAGTTTGTCAAATACCCCAA199930.23641796918466249No Hit
CCTCGACACAAGGGTTTGT198540.23477428901076824No Hit
TGAGGTAGTAGTTTGTGCTGTT186440.22046599397183253No Hit
CGCGACCTCAGATCAGACGTGGCG164180.1941434611150797No Hit
CTTCTCACTACTGCACTTGACTAG163450.1932802333978547No Hit
TACCTGGTTGATCCTGCC158660.1876160405683917No Hit
CTCGACACAAGGGTTTGT155610.1840094042156021No Hit
TTATAAAGCAATGAGACTGATT150050.17743468352002506No Hit
CTCCCACTGCTTCACTTGACTAG149930.1772927830733579No Hit
CCAAGTCCTTCTGATCGAGGCCC146610.1733668707155673No Hit
TATTGCACTTGTCCCGGCCTGTT145090.17156946505778364No Hit
ACTGGACTTGGAGTCAGAAGG144760.17117923882944902No Hit
TTCACAGTGGCTAAGTTCTG141340.1671350760994358No Hit
CAACGGAATCCCAAAAGCAGCT140170.16575154674443127No Hit
AGCAGCATTGTACAGGGCTATG139210.16461634317109422No Hit
CCAAGTCCTTCTGATCGAGGCC136970.16196753483330778No Hit
CCCCCCACTGCTAAATTTGACTGG131300.15526273872828583No Hit
TAGCTTATCAGACTGATGTTGACA130630.15447046123439437No Hit
CAAGTCCTTCTGATCGAGGCCC127810.15113580073771676No Hit
TGAGGTAGTAGGTTGTGTGGTT127810.15113580073771676No Hit
CTGCGTTCGCGCTTTCCCCTG127040.1502252728716027No Hit
ACTGGACTTGGAGTCAGAAGGCA123400.1459209593226997No Hit
TGTCAGTTTGTCAAATACCCCA119010.14072976798212716No Hit
ACCACAGGGTAGAACCACGGAC115620.13672108036378072No Hit
AACGGAATCCCAAAAGCAGCTG112090.13254684222432261No Hit
TAGCAGCACGTAAATATTGGC111840.13225121629376607No Hit
AGCTACATTGTCTGCTGGGTTT110400.13054841093376052No Hit
AGCAGCATTGTACAGGGCTATGT108900.12877465535042137No Hit
CCTGTCTGAGCGTCGCTT108510.1283134788987532No Hit
TTCAAGTAATCCAGGATAGGC108480.12827800378708643No Hit
TTCAAGTAATCCAGGATAGGCTT107920.1276158017026398No Hit
CAAAGAATTCTCCTTTTGGGCT107410.1270127248043045No Hit
ATCCCACCGCTGCCACCA106930.12644512301763597No Hit
CAAGTCCTTCTGATCGAGGCC106080.1254399948537438No Hit
AGCTCGGTCTGAGGCCCCTCAGT98370.11632289115538062No Hit
CACTAGATTGTGAGCTCCTGGA97380.1151522124703768No Hit
TATTGCACTTGTCCCGGCCTG96820.1144900103859302No Hit
CGCGACCTCAGATCAGACGTGG96540.11415890934370687No Hit
TGTAAACATCCTACACTCAGCT96410.11400518385981748No Hit
GGGCTACGCCTGTCTGAGCGTCGCT96190.11374503304092776No Hit
CAAAGTGCTGTTCGTGCAGGT96160.11370955792926096No Hit
TGAGGTAGTAGGTTGTATAGT95850.11334298177537087No Hit
ACCCCCCACTGCTAAATTTGACTG93990.11114352485203036No Hit
TATTGCACTTGTCCCGGCCTGTAG91680.10841194125368808No Hit
TAGCAGCACGTAAATATTGGCGT91590.10830551591868773No Hit
AATGACACGATCACTCCCGTTG91360.10803354006257573No Hit
CAAAGTGCTGTTCGTGCAGGTAG89980.10640168492590374No Hit
CCCCCACTGCTAAATTTGACTGGCTT88020.1040839776303406No Hit
TGTAAACATCCCCGACTGGAAGC87040.10292512398255901No Hit
TGAAAAGAACTTTGAAGAGAG84850.10033544083088387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)