FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8850847
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG125760614.208877410263673No Hit
CCCCCCACTGCTAAATTTGACTG6574627.428238223980145No Hit
TTCAAGTAATCCAGGATAGGCT3232153.6517973929500758No Hit
TCGTACGACTCTTAGCGG3075473.4747747870909977No Hit
CCCCCACTGCTAAATTTGACTG2618852.958869360186658No Hit
AACATTCAACGCTGTCGGTGAG1971072.2269846038463887No Hit
TAGCAGCACGTAAATATTGGCG1933042.184016964704056No Hit
TGAGGTAGTAGATTGTATAGTT1704161.9254202450906674No Hit
TAGCTTATCAGACTGATGTTGAC1689231.9085518030082318No Hit
TAGCTTATCAGACTGATGTTGA1508781.7046730103909828No Hit
AAGCTGCCAGTTGAAGAACTGT1450951.639334630911595No Hit
TAGCTTATCAGACTGATGTTGACT1105551.2490894939207513No Hit
TATTGCACTTGTCCCGGCCTGTAA896811.0132476586704076No Hit
TATTGCACTTGTCCCGGCCTGT867530.9801660790204598No Hit
TGAGGTAGTAGGTTGTATAGTT837050.9457286969258423No Hit
TATTGCACTTGTCCCGGCCTGTAT726830.8211982423829041No Hit
TTCAAGTAATTCAGGATAGGTT620810.701413096396311No Hit
TGTAAACATCCTTGACTGGAAGCT581090.6565360354777344No Hit
CATAAAGTAGAAAGCACTACT572390.6467064677538772No Hit
TGAGGTAGTAGTTTGTACAGTT535930.6055126701433208No Hit
TGAGAACTGAATTCCATGGGTT531680.6007108698184479No Hit
TATTGCACTTGTCCCGGCCTGTA526860.5952650633323567No Hit
AACATTCAACGCTGTCGGTGAGT521310.5889944770257581No Hit
CAACGGAATCCCAAAAGCAGCTGT484550.5474617288040342No Hit
AGCAGCATTGTACAGGGCTATGA470880.5320168792885019No Hit
TTCTCACTACTGCACTTGACTAG443460.5010367934277928No Hit
CATTGCACTTGTCTCGGTCTGA439400.496449661823326No Hit
GCCTGTCTGAGCGTCGCT436630.4933200178468795No Hit
CAACGGAATCCCAAAAGCAGCT424400.4795021312649512No Hit
CCCCCACTGCTAAATTTGACTGGCTT393290.4443529528868819No Hit
TCGTTTCCCGGCCAATGCACCA363260.4104239967090155No Hit
CCCATAAAGTAGAAAGCACT348320.3935442562728742No Hit
CCCATAAAGTAGAAAGCACTAC333900.3772520302294232No Hit
CTCCCACTGCTTCACTTGACTAG315350.3562935841055664No Hit
TGTAAACATCCCCGACTGGAAGCT283090.31984509505135494No Hit
TTCACAGTGGCTAAGTTCCGC270940.3061175952990714No Hit
AACGGAATCCCAAAAGCAGCTG269790.3048182846229293No Hit
ACTGGACTTGGAGTCAGAAGGC262950.29709021068831043No Hit
CAACACCAGTCGATGGGCTGT258050.29155401737257464No Hit
TAGCTTATCAGACTGATGTTGACA240910.272188639121205No Hit
CTTCTCACTACTGCACTTGACTAG240230.27142035106922535No Hit
TGATGTAGTAGATTGTATAGTT230720.2606756166952157No Hit
TAGCAGCACGTAAATATTGGC221300.2500325675045564No Hit
CAAAGAATTCTCCTTTTGGGCT217390.2456149112056733No Hit
CCCATAAAGTAGAAAGCACTA201960.2281815514379584No Hit
TGAGGTAGTAGATTGTATAGT200020.22598967081907528No Hit
ACCCCCCACTGCTAAATTTGACTG191230.21605841791186767No Hit
TTAATGCTAATCGTGATAGGGGTT183960.20784451476790863No Hit
GGAATACCGGGTGCTGTAGGCTTT179430.20272636053927945No Hit
TGTCAGTTTGTCAAATACCCCA178400.2015626301076044No Hit
TTCACAGTGGCTAAGTTCTG176480.19939334619613241No Hit
CACCCGGCTGTGTGCACATGTGC172340.19471582776202095No Hit
TGAGAACTGAATTCCATAGGCTGT170250.19235447183755408No Hit
GGAATACCGGGTGCTGTAGGCTT169520.1915296920170465No Hit
TTCACAGTGGCTAAGTTCTGC162640.18375642466760525No Hit
AGAATTACTCTGAGACCT159430.18012965312811305No Hit
TTCACAGTGGCTAAGTTCCG158960.17959863050395064No Hit
CTCCCACTGCTTCACTTGACTAGCCT157490.1779377725092299No Hit
TCGTTTCCCGGCCAACGCACCA153870.17384776846780878No Hit
TGTAAACATCCCCGACTGGA146730.16578074392202238No Hit
TTCAAGTAATCCAGGATAGGC143690.16234604439552508No Hit
CACTAGATTGTGAGCTCCTGGA137710.15558962887958633No Hit
ACCGGGTGCTGTAGGCTT135370.15294581411247984No Hit
CCCCCCACTGCTAAATTTGACTGG129400.14620069695024668No Hit
TGTAAACATCCTACACTCTCAGCT129230.14600862493725175No Hit
TGAGGTAGTAGTTTGTGCTGTT127070.1435681805368458No Hit
TGGCTCAGTTCAGCAGGAAC126480.1429015776682164No Hit
CCCCCCACTGCTAAATTTGACTGGCT124180.14030295631593223No Hit
TATTGCACTTGTCCCGGCCTGTT122310.13819016417298818No Hit
CACTAGATTGTGAGCTCCTGGAA121010.13672137819126237No Hit
TGAGGTAGTAAGTTGTATTGTT120920.13661969300791213No Hit
TCCCACTGCTTCACTTGACTAG119240.13472156958537415No Hit
TGATGTAGTAGGTTGTATAGTT114090.12890291742699878No Hit
CAAAGTGCTGTTCGTGCAGGTAG112980.12764880016567906No Hit
ATGCACCTGGGCAAGGATTCTGA109860.12412371380953709No Hit
TTCAAGTAATCCAGGATAGGCTT106890.1207681027589789No Hit
AGCTCGGTCTGAGGCCCCTCAGT106790.12065511922192305No Hit
ATCACATTGCCAGGGATTTCC105290.11896036616608557No Hit
AGCAGCATTGTACAGGGCTATGT104750.11835025506598408No Hit
TCCTGTCTGAGCGTCGCT104300.11784182914923284No Hit
ACTGGACTTGGAGTCAGAAGGCT102130.11539008639512129No Hit
TTATAAAGCAATGAGACTGATT101880.11510762755248169No Hit
TATTGCACTTGTCCCGGCCTGTAG98680.11149215436669507No Hit
CAAAGAATTCTCCTTTTGGGCTT97750.11044140747207584No Hit
CAAAGAATTCTCCTTTTGGGC96470.1089952181977612No Hit
TCGATTCCCGGCCAACGCACCA93680.10584297751390347No Hit
AATCCTTGGAACCTAGGTGTGAGT91450.10332344463755841No Hit
TACCACCCTGAACGCGCCCGA89830.10149311133725394No Hit
TGAGGTAGTAGATTGTATAGTTA88610.10011471218517279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)