FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11282640
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9980808.846156573284267No Hit
GGAATACCGGGTGCTGTAGGCTTT7588256.725597909709075No Hit
GGAATACCGGGTGCTGTAGGCTT4651114.122359660504988No Hit
TAGCAGCACGTAAATATTGGCG4202743.7249615338254167No Hit
AAGCTGCCAGTTGAAGAACTGT4140783.670045308544809No Hit
TTCAAGTAATCCAGGATAGGCT4089383.6244885948678673No Hit
AACATTCAACGCTGTCGGTGAGT3943223.494944445626201No Hit
TGAATACCGGGTGCTGTAGGCTTT3244252.8754351818368753No Hit
CCCCCCACTGCTAAATTTGACTG3135982.7794735983776846No Hit
AACATTCAACGCTGTCGGTGAG2451922.1731793268242185No Hit
TGAATACCGGGTGCTGTAGGCTT1989571.7633904830784284No Hit
TATTGCACTTGTCCCGGCCTGTAA1838981.6299199478136321No Hit
TAGCTTATCAGACTGATGTTGACT1735691.5383722249402623No Hit
TATTGCACTTGTCCCGGCCTGTAT1704001.5102848269553935No Hit
TAGCTTATCAGACTGATGTTGAC1629401.4441655499067594No Hit
GGAATACCGGGTGCTGTAGGCTTA1158481.0267809661568568No Hit
TGTAAACATCCTTGACTGGAAGCT1115280.9884920550509456No Hit
TGAGGTAGTAGGTTGTATAGTT1101880.9766154020690193No Hit
CCCCCACTGCTAAATTTGACTG1070830.9490952472116456No Hit
TAGCTTATCAGACTGATGTTGA951630.8434462147157049No Hit
CATAAAGTAGAAAGCACTACT936270.8298323796558251No Hit
TATTGCACTTGTCCCGGCCTGT833300.7385682783462026No Hit
CAACGGAATCCCAAAAGCAGCTGT806850.715125183467699No Hit
GGAATACCGGGTGCTGTAGGCT704620.6245169570242426No Hit
AGCAGCATTGTACAGGGCTATGA664970.589374472641155No Hit
TGAGGTAGTAGATTGTATAGTT634390.5622708869555352No Hit
TTCAAGTAATTCAGGATAGGTT623670.5527695645699943No Hit
TGTAAACATCCCCGACTGGAAGCT619240.5488431785468649No Hit
CCCATAAAGTAGAAAGCACTAC582250.5160582984124283No Hit
TATTGCACTTGTCCCGGCCTGTA564070.4999450483220239No Hit
TGAATACCGGGTGCTGTAGGCTTA505540.44806889167783426No Hit
GCCTGTCTGAGCGTCGCT488930.433347159884566No Hit
TGTAAACATCCTACACTCTCAGCT477350.42308360454645366No Hit
CCCATAAAGTAGAAAGCACT409820.3632305914218658No Hit
ACTGGACTTGGAGTCAGAAGGC387750.343669566697156No Hit
CACCCGGCTGTGTGCACATGTGC346990.30754327001481924No Hit
TGTCAGTTTGTCAAATACCCCAA324940.28799997163784363No Hit
CATTGCACTTGTCTCGGTCTGA318520.2823098140151596No Hit
CAAAGAATTCTCCTTTTGGGCT305340.27062815085830977No Hit
TGAATACCGGGTGCTGTAGGCT300760.2665688172271738No Hit
TGAGGTAGTAGTTTGTACAGTT298930.26494685640949284No Hit
TTCTCACTACTGCACTTGACTAG295640.2620308722072139No Hit
CTTCTCACTACTGCACTTGACTAG258480.22909531811703643No Hit
AACATTCAACGCTGTCGGTGAGTT257190.22795196868817935No Hit
TCGTACGACTCTTAGCGG245760.21782136095807367No Hit
CAACGGAATCCCAAAAGCAGCT241240.21381520637014031No Hit
CAAAGAATTCTCCTTTTGGGCTT240720.21335432132905066No Hit
CCCCCACTGCTAAATTTGACTGGCTT238380.2112803386441471No Hit
TAGCTTATCAGACTGATGTTGACA235970.20914431374217382No Hit
CAACACCAGTCGATGGGCTGT233030.20653854062524374No Hit
TAGCAGCACGTAAATATTGGC230750.2045177369835429No Hit
TGATGTAGTAGGTTGTATAGTT208420.1847262697382882No Hit
CCCCCCACTGCTAAATTTGACTGGCT203310.18019718789219544No Hit
CTCCCACTGCTTCACTTGACTAG202490.1794704076350925No Hit
TATTGCACTTGTCCCGGCCTGTAG201580.17866385881318556No Hit
AACGGAATCCCAAAAGCAGCTG201240.17836251090170385No Hit
TGTCAGTTTGTCAAATACCCCA201010.17815865790276036No Hit
GGAATACCGGGTGCTGTAGGCTA193630.1716176355888338No Hit
CGCCTGTCTGAGCGTCGCT193050.17110357150454147No Hit
CGAATACCGGGTGCTGTAGGCTTT187510.16619337318216304No Hit
TTCAAGTAATCCAGGATAGGCTT185390.16431438032233592No Hit
TCCTGTCTGAGCGTCGCT181620.16097296377443576No Hit
TGTAAACATCCCCGACTGGAAGC175510.1555575645416321No Hit
TTCAAGTAATCCAGGATAGGC175040.15514099536987797No Hit
AGCTCGGTCTGAGGCCCCTCAGT175020.15512326902214377No Hit
TTCACAGTGGCTAAGTTCCGC172570.15295179142470203No Hit
TATTGCACTTGTCCCGGCCTGTAAT171160.15170208390943965No Hit
TGAGGTAGTAGTTTGTGCTGTT167240.14822771975353286No Hit
CACTAGATTGTGAGCTCCTGGAA165960.1470932334985429No Hit
TGTAAACATCCTACACTCAGCT164510.14580807328781206No Hit
TATTGCACTTGTCCCGGCCTGTT161480.14312253160607802No Hit
CACTAGATTGTGAGCTCCTGGA158450.14043698992434395No Hit
AACCGGGCGGAAACACCA157240.13936454588642375No Hit
AACATTCAACGCTGTCGGTGA148420.1315472265356335No Hit
TTCACAGTGGCTAAGTTCTGC142960.12670793360419194No Hit
TATTGCACTTGTCCCGGCCTGTTT141020.12498847787397276No Hit
CTACGCCTGTCTGAGCGTCGCT139870.1239692128792552No Hit
TAGCAGCACATCATGGTTTACA137150.12155842958740153No Hit
ATCACATTGCCAGGGATTTCC133050.1179245283018868No Hit
TATTGCACTTGTCCCGGCCTGTAAA131660.11669254713435862No Hit
TGAGGTAGTAGGTTGTGTGGTT129320.11461856444945509No Hit
TTCACAGTGGCTAAGTTCCG126070.11173803294264463No Hit
TGATGTAGTAGATTGTATAGTT121760.10791800500592061No Hit
CCCCCCACTGCTAAATTTGACTGG119240.1056844851914091No Hit
AACATTCAACGCTGTCGGTGAGA118740.10524132649805365No Hit
CAAAGTGCTGTTCGTGCAGGT117590.1042220615033361No Hit
AGCAGCATTGTACAGGGCTATGT116010.10282168003233286No Hit
ATGCACCTGGGCAAGGATTCTGA115730.1025735111640538No Hit
CGAATACCGGGTGCTGTAGGCTT115180.1020860366013628No Hit
ACTGGACTTGGAGTCAGAAGGCT114550.10152765664773493No Hit
ATCACATTGCCAGGGATTTCCA113870.10092496082477151No Hit
CAAAGTGCTGTTCGTGCAGGTAG113760.10082746591223331No Hit
CCCATAAAGTAGAAAGCACTA112850.10002091709032637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)