FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8762502
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG91990310.498177347063658No Hit
AAGCTGCCAGTTGAAGAACTGT3625544.137562536362331No Hit
AACATTCAACGCTGTCGGTGAGT3337923.8093229536495397No Hit
TAGCAGCACGTAAATATTGGCG3004863.429226036125298No Hit
TTCAAGTAATCCAGGATAGGCT2944193.3599878208301694No Hit
CCCCCCACTGCTAAATTTGACTG2598832.965853816638216No Hit
AACATTCAACGCTGTCGGTGAG2200012.510709840636841No Hit
TATTGCACTTGTCCCGGCCTGTAA1984502.264764105046709No Hit
TATTGCACTTGTCCCGGCCTGTAT1943982.218521604902344No Hit
TAGCTTATCAGACTGATGTTGACT1835082.094242032697967No Hit
TAGCTTATCAGACTGATGTTGAC1762592.011514519483134No Hit
GCCTGTCTGAGCGTCGCT1360471.5526044958392022No Hit
TGAGGTAGTAGGTTGTATAGTT1170071.3353149591292532No Hit
TGTAAACATCCTTGACTGGAAGCT1080391.232969761376374No Hit
TAGCTTATCAGACTGATGTTGA1080241.2327985773926216No Hit
TATTGCACTTGTCCCGGCCTGT984211.1232065909942162No Hit
CCCCCACTGCTAAATTTGACTG883591.0083763746929815No Hit
CATAAAGTAGAAAGCACTACT863280.9851980632928815No Hit
TATTGCACTTGTCCCGGCCTGTA779350.889414918250518No Hit
AGCAGCATTGTACAGGGCTATGA735550.8394291949947629No Hit
TGAGGTAGTAGATTGTATAGTT732000.8353778407126184No Hit
CAACGGAATCCCAAAAGCAGCTGT651020.7429613140173891No Hit
CCCATAAAGTAGAAAGCACTAC638420.7285818593821719No Hit
TGTAAACATCCCCGACTGGAAGCT595140.6791895739367592No Hit
TTCAAGTAATTCAGGATAGGTT580150.6620825878270841No Hit
ACTGGACTTGGAGTCAGAAGGC480880.5487930273796228No Hit
CAACGGAATCCCAAAAGCAGCT454220.5183679273339966No Hit
CACCCGGCTGTGTGCACATGTGC451510.5152752033608665No Hit
CCCATAAAGTAGAAAGCACT365330.4169242985622143No Hit
TCGTACGACTCTTAGCGG341300.38950062436505006No Hit
TGTCAGTTTGTCAAATACCCCAA341160.389340852646881No Hit
CATTGCACTTGTCTCGGTCTGA339300.38721817124834895No Hit
TGAGGTAGTAGTTTGTACAGTT334440.38167181017476515No Hit
TCCTGTCTGAGCGTCGCT324500.37032801818476047No Hit
TGTAAACATCCTACACTCTCAGCT320060.36526097226568394No Hit
TTAATGCTAATCGTGATAGGGGTT281350.3210840921919333No Hit
TAGCTTATCAGACTGATGTTGACA259590.29625100228222484No Hit
AACATTCAACGCTGTCGGTGAGTT240240.2741682683781413No Hit
CAACACCAGTCGATGGGCTGT234310.26740079488712243No Hit
CAAAGAATTCTCCTTTTGGGCT232780.2656547182528461No Hit
TATTGCACTTGTCCCGGCCTGTAG226690.2587046485124911No Hit
TCGTTTCCCGGCCAATGCACCA223630.2552124952439383No Hit
CGCCTGTCTGAGCGTCGCT222910.25439081212192594No Hit
TGTCAGTTTGTCAAATACCCCA221510.2527930949402351No Hit
CCCCCACTGCTAAATTTGACTGGCTT203190.23188582439125266No Hit
AACGGAATCCCAAAAGCAGCTG200120.22838225885711635No Hit
TGAGGTAGTAGTTTGTGCTGTT199880.22810836448311222No Hit
TATTGCACTTGTCCCGGCCTGTT198070.2260427444124977No Hit
CAAAGAATTCTCCTTTTGGGCTT197830.22576885003849356No Hit
TAGCAGCACGTAAATATTGGC196450.22419395738796977No Hit
CACTAGATTGTGAGCTCCTGGA195440.2230413185640357No Hit
TTCTCACTACTGCACTTGACTAG194560.22203703919268722No Hit
ATCCCGGACGAGCCCCCA189810.21661621304052198No Hit
GGAATACCGGGTGCTGTAGGCTTT189800.2166048007749385No Hit
CACCCGTAGAACCGACCTTGCG180350.20582020979852556No Hit
CACTAGATTGTGAGCTCCTGGAA178700.20393718597724714No Hit
CTTCTCACTACTGCACTTGACTAG172960.19738654553231486No Hit
TTCAAGTAATCCAGGATAGGCTT171840.1961083717869622No Hit
TTCACAGTGGCTAAGTTCCGC169920.19391721679492913No Hit
TATTGCACTTGTCCCGGCCTGTAAT166230.1897060907946155No Hit
TATTGCACTTGTCCCGGCCTGTTT165840.18926101243685878No Hit
CTCCCACTGCTTCACTTGACTAG163270.18632806018189782No Hit
AGCAGCATTGTACAGGGCTATGT163050.1860769903390607No Hit
TGTAAACATCCCCGACTGGAAGC160060.18266472292959246No Hit
ACTGGACTTGGAGTCAGAAGGCA158970.18142078598099035No Hit
TGAGAACTGAATTCCATGGGTT157440.17967470934671398No Hit
TCGTTTCCCGGGCGGCGCACCA149660.17079596672274652No Hit
TGAGAACTGAATTCCATAGGCTGT148630.16962050336764545No Hit
CCCCCCACTGCTAAATTTGACTGGCT147900.16878740798004954No Hit
TTATAAAGCAATGAGACTGATT144240.16461051877648644No Hit
TATTGCACTTGTCCCGGCCTGTAAA138180.157694685832882No Hit
TTCACAGTGGCTAAGTTCTGC136810.15613120544794168No Hit
CAACGGAATCCCAAAAGCAGCTGA135370.15448783920391687No Hit
ACTGGACTTGGAGTCAGAAGGCT133240.15205702663463017No Hit
AGCTCGGTCTGAGGCCCCTCAGT130290.14869040828749597No Hit
ACCACAGGGTAGAACCACGGAC128620.14678455993505052No Hit
CCCATAAAGTAGAAAGCACTA126770.14467329080210195No Hit
TGATGTAGTAGGTTGTATAGTT126640.14452493134951638No Hit
CAAAGTGCTGTTCGTGCAGGTAG126050.14385160768008953No Hit
TCAGTGCACTACAGAACTTTGT124870.1425049603412359No Hit
TTCACAGTGGCTAAGTTCCG124240.14178598760947503No Hit
TTCAAGTAATCCAGGATAGGC123320.1407360591757925No Hit
TGAGGTAGTAGGTTGTGTGGTT123020.14039369120828732No Hit
GGAATACCGGGTGCTGTAGGCTT121850.13905845613501716No Hit
ATGCACCTGGGCAAGGATTCTGA115940.13231380717516528No Hit
AACCGGGCGGAAACACCA110100.12564904407439795No Hit
CTCCCACTGCTTCACTTGACTAGCCT106640.12170040018250494No Hit
CTACGCCTGTCTGAGCGTCGCT104950.11977172729889249No Hit
TATTGCACTTGTCCCGGCCTGA104500.11925817534763473No Hit
ATCACATTGCCAGGGATTTCC104310.11904134230154811No Hit
TAGCAGCACGTAAATATTGGCGT104200.11891580738012957No Hit
TAGCAGCACATCATGGTTTACA103950.1186305007405419No Hit
TGTCAGTTTGTCAAATACCCC101090.11536659278365928No Hit
TGAGGTAGTAGGTTGTATAGT100930.11518399653432318No Hit
CAAAGTGCTGTTCGTGCAGGT99240.11325532365071073No Hit
AGCAGCATTGTACAGGGCTATG98040.11188585178069003No Hit
AGCTACATCTGGCTACTGGGTCTC93960.10722964742261971No Hit
ATCACATTGCCAGGGATTTCCA93920.10718399836028566No Hit
AACATTCAACGCTGTCGGTGAGA92750.10584876328701551No Hit
TGTAAACATCCTACACTCAGCT90580.10337230165539477No Hit
ACTGGACTTGGAGTCAGAAGG90130.10285874970413701No Hit
TACGCCTGTCTGAGCGTCGCT88960.10152351463086685No Hit
AATGCACCCGGGCAAGGATTCT88610.10112408533544415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)