FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8370911
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG115006513.738827231588054No Hit
AACATTCAACGCTGTCGGTGAGT4418915.27888780564027No Hit
AAGCTGCCAGTTGAAGAACTGT3994814.772252386866853No Hit
TTCAAGTAATCCAGGATAGGCT3467444.142249272510483No Hit
AACATTCAACGCTGTCGGTGAG2817263.3655357224560145No Hit
TAGCAGCACGTAAATATTGGCG2741753.275330486729581No Hit
CCCCCCACTGCTAAATTTGACTG2196922.6244694275210905No Hit
TATTGCACTTGTCCCGGCCTGTAA1692562.0219543607619292No Hit
TATTGCACTTGTCCCGGCCTGTAT1497351.7887539361008615No Hit
TAGCTTATCAGACTGATGTTGACT1396551.6683369348927495No Hit
TGAGGTAGTAGGTTGTATAGTT1356881.6209466329292No Hit
TAGCTTATCAGACTGATGTTGAC1273681.5215548224082183No Hit
CATAAAGTAGAAAGCACTACT1186511.41742039785156No Hit
TATTGCACTTGTCCCGGCCTGT1021011.219711928606098No Hit
CCCCCACTGCTAAATTTGACTG1019451.2178483321588296No Hit
TAGCTTATCAGACTGATGTTGA978651.1691081173841176No Hit
TGTAAACATCCTTGACTGGAAGCT744050.888851882429523No Hit
CAACGGAATCCCAAAAGCAGCTGT717940.8576605341999216No Hit
TGAGGTAGTAGATTGTATAGTT674650.8059457327882235No Hit
CCCATAAAGTAGAAAGCACTAC640340.7649585570793908No Hit
CCCATAAAGTAGAAAGCACT567860.6783729990678434No Hit
TATTGCACTTGTCCCGGCCTGTA564660.6745502371247287No Hit
AGCAGCATTGTACAGGGCTATGA538530.6433349966329829No Hit
TTCAAGTAATTCAGGATAGGTT518440.6193352193088661No Hit
CGCCTGTCTGAGCGTCGCT490330.5857546448648182No Hit
GCCTGTCTGAGCGTCGCT454580.5430472262815839No Hit
TGTAAACATCCCCGACTGGAAGCT432920.5171719063791265No Hit
TCGTACGACTCTTAGCGG419840.5015463669366452No Hit
CAACACCAGTCGATGGGCTGT410150.4899705659276511No Hit
CATTGCACTTGTCTCGGTCTGA389180.4649195290691778No Hit
CACCCGGCTGTGTGCACATGTGC370270.44232939521158454No Hit
ACTGGACTTGGAGTCAGAAGGC366390.437694296355558No Hit
TTAATGCTAATCGTGATAGGGGTT350470.4186760556885625No Hit
TGAGGTAGTAGTTTGTACAGTT326940.39056680927559734No Hit
TGATGTAGTAGGTTGTATAGTT263780.3151150454233715No Hit
ATCCCGGACGAGCCCCCA261620.3125346811117691No Hit
AACATTCAACGCTGTCGGTGAGTT252490.30162786344282005No Hit
TGTAAACATCCTACACTCTCAGCT252290.30138894082137535No Hit
TGTCAGTTTGTCAAATACCCCAA237900.2841984582084316No Hit
AACGGAATCCCAAAAGCAGCTG236440.28245432307188545No Hit
TGAGGTAGTAGTTTGTGCTGTT193780.23149212791773796No Hit
TATTGCACTTGTCCCGGCCTGTAG173370.2071100743993097No Hit
TTCACAGTGGCTAAGTTCCGC172850.20648887558355355No Hit
TCCTGTCTGAGCGTCGCT168330.20108922433890408No Hit
TATTGCACTTGTCCCGGCCTGTT168010.20070694814459264No Hit
TTCAAGTAATCCAGGATAGGCTT162030.19356316176339708No Hit
TAGCAGCACGTAAATATTGGC158340.18915503939774297No Hit
TTCAAGTAATCCAGGATAGGC155590.1858698533528788No Hit
TGAGGTAGTAGGTTGTGTGGTT152290.18192763009904178No Hit
CACTAGATTGTGAGCTCCTGGA152200.1818201149193917No Hit
TGAGAACTGAATTCCATGGGTT146040.17446129817889594No Hit
TAGCTTATCAGACTGATGTTGACA145580.1739117761495732No Hit
CAAAGAATTCTCCTTTTGGGCT143430.17134335796904304No Hit
TTATAAAGCAATGAGACTGATT139930.16716221209376136No Hit
CAACGGAATCCCAAAAGCAGCT139620.16679188203052214No Hit
ATCCCACCGCTGCCACCA139360.16648128262264406No Hit
AACATTCAACGCTGTCGGTGA139170.16625430613227163No Hit
ACCACAGGGTAGAACCACGGAC137640.1644265480782199No Hit
CTACGCCTGTCTGAGCGTCGCT135570.16195369894626763No Hit
TTCTCACTACTGCACTTGACTAG134810.1610457929847779No Hit
TGATGTAGTAGATTGTATAGTT132120.1578322837263471No Hit
CAAAGAATTCTCCTTTTGGGCTT127370.15215787146703627No Hit
TGTCAGTTTGTCAAATACCCCA126950.15165613396200248No Hit
CCCATAAAGTAGAAAGCACTA126310.1508915815733795No Hit
AGCTCGGTCTGAGGCCCCTCAGT126030.150557089903357No Hit
TTCACAGTGGCTAAGTTCTGC124390.1485979244075107No Hit
AACATTCAACGCTGTCGGTGAGA123420.14743914969350408No Hit
TTCACAGTGGCTAAGTTCCG120940.1444765091875902No Hit
TGAGGTAGTAGGTTGTATAGT119390.14262485887139403No Hit
CACTAGATTGTGAGCTCCTGGAA119020.1421828520217214No Hit
TATTGCACTTGTCCCGGCCTGTAAT118060.14103602343878702No Hit
TTCACAGTGGCTAAGTTCTG112400.13427451325190293No Hit
AGCAGCATTGTACAGGGCTATGT112010.13380861414008582No Hit
CAAAGTGCTGTTCGTGCAGGT111240.13288876204752387No Hit
CCCCCACTGCTAAATTTGACTGGCTT108920.13011725963876572No Hit
TACGCCTGTCTGAGCGTCGCT106580.12732186496786313No Hit
ACTGGACTTGGAGTCAGAAGGCT105900.12650952805495125No Hit
ACGCCTGTCTGAGCGTCGCT104500.12483706970483857No Hit
TGTAAACATCCCCGACTGGAAGC104230.12451452416588828No Hit
TATTGCACTTGTCCCGGCCTGTAAA103680.12385748695691544No Hit
AGAGGTAGTAGGTTGCATAGTT101200.12089484645100158No Hit
TATTGCACTTGTCCCGGCCTGTTT99520.11888789643086638No Hit
TCCTGTACTGAGCTGCCCCGAG95740.11437225888556216No Hit
AGCAGCATTGTACAGGGCTATG94350.11271174666652173No Hit
CAAAGTGCTGTTCGTGCAGGTAG93970.11225779368577685No Hit
TCCTGTACTGAGCTGCCCCGAGT93900.11217417076827123No Hit
TGAGGTAGTAAGTTGTATTGTT93510.11170827165645411No Hit
TGAGAACTGAATTCCATAGGCTGT93300.11145740290393721No Hit
TCAGTGCACTACAGAACTTTGT93220.11136183385535936No Hit
AACCGGGCGGAAACACCA90820.10849476239802336No Hit
CTCCCACTGCTTCACTTGACTAG90060.10758685643653361No Hit
ACTGGACTTGGAGTCAGAAGGCA87650.10470783884812537No Hit
ATCACATTGCCAGGGATTTCC85910.10262921204155677No Hit
CTTCTCACTACTGCACTTGACTAG84340.10075366946321614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)