FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8750042
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG91859310.498155323140162No Hit
AACATTCAACGCTGTCGGTGAGT3949924.514172617685721No Hit
TAGCAGCACGTAAATATTGGCG3388433.872472840701793No Hit
AAGCTGCCAGTTGAAGAACTGT3299573.770919042445739No Hit
TTCAAGTAATCCAGGATAGGCT2939023.3588638774533885No Hit
CCCCCCACTGCTAAATTTGACTG2930873.3495496364474597No Hit
AACATTCAACGCTGTCGGTGAG2886103.2983841677559944No Hit
TATTGCACTTGTCCCGGCCTGTAA2367792.7060327253286327No Hit
TATTGCACTTGTCCCGGCCTGTAT2351602.6875299569990636No Hit
TAGCTTATCAGACTGATGTTGACT2136902.442159706204839No Hit
TAGCTTATCAGACTGATGTTGAC1754152.004733234423332No Hit
TATTGCACTTGTCCCGGCCTGT1110691.2693539071012458No Hit
TAGCTTATCAGACTGATGTTGA1105281.2631710796359605No Hit
TGAGGTAGTAGGTTGTATAGTT1088861.2444054554252424No Hit
TGTAAACATCCTTGACTGGAAGCT1060681.2121998957262148No Hit
TATTGCACTTGTCCCGGCCTGTA1019071.1646458382714049No Hit
CCCCCACTGCTAAATTTGACTG1015211.1602344308747319No Hit
CATAAAGTAGAAAGCACTACT770670.8807614866305784No Hit
AGCAGCATTGTACAGGGCTATGA745530.852030195969345No Hit
TGTAAACATCCCCGACTGGAAGCT667510.7628649096770049No Hit
TGAGGTAGTAGATTGTATAGTT665140.760156351249514No Hit
CAACGGAATCCCAAAAGCAGCTGT554840.6340998134637525No Hit
CCCATAAAGTAGAAAGCACTAC550780.6294598357356457No Hit
TTCAAGTAATTCAGGATAGGTT537650.6144541934770141No Hit
ACTGGACTTGGAGTCAGAAGGC510860.5838371975814516No Hit
TTAATGCTAATCGTGATAGGGGTT505730.5779743685801737No Hit
TGAGAACTGAATTCCATAGGCTGT408550.46691204453647195No Hit
CCCATAAAGTAGAAAGCACT378720.43282077960311505No Hit
CATTGCACTTGTCTCGGTCTGA363590.41552943403014525No Hit
ATCCCGGACGAGCCCCCA354640.405300911698481No Hit
CACCCGGCTGTGTGCACATGTGC348450.3982266599406037No Hit
TGTCAGTTTGTCAAATACCCCAA337500.3857124342946011No Hit
CAACACCAGTCGATGGGCTGT328540.3754724834463652No Hit
TATTGCACTTGTCCCGGCCTGTAG322170.36819251839019745No Hit
AACATTCAACGCTGTCGGTGAGTT321260.36715252338217347No Hit
TGAGAACTGAATTCCATGGGTT315710.3608096966848845No Hit
TGTAAACATCCTACACTCTCAGCT291340.33295840179967134No Hit
TGAGGTAGTAGTTTGTACAGTT291000.3325698322362338No Hit
CAAAGAATTCTCCTTTTGGGCT286780.32774699824297987No Hit
CACTAGATTGTGAGCTCCTGGAA286360.32726700054696883No Hit
CGCCTGTCTGAGCGTCGCT275770.3151642014975471No Hit
CACTAGATTGTGAGCTCCTGGA273800.3129127837329238No Hit
TATTGCACTTGTCCCGGCCTGTT259700.29679857536683824No Hit
TAGCTTATCAGACTGATGTTGACA247960.28338149691167197No Hit
TTCACAGTGGCTAAGTTCCG241400.2758843900406421No Hit
AACGGAATCCCAAAAGCAGCTG217600.24868452060001542No Hit
TATTGCACTTGTCCCGGCCTGTAAT202770.23173603052419633No Hit
TCGTACGACTCTTAGCGG202760.23172460200762465No Hit
TATTGCACTTGTCCCGGCCTGTTT198000.22628462811949934No Hit
TGTCAGTTTGTCAAATACCCCA197710.22595320113892023No Hit
TAGCAGCACGTAAATATTGGC197440.22564463119148456No Hit
AGCTCGGTCTGAGGCCCCTCAGT195540.2234732130428631No Hit
TCGTTTCCCGGGCGGCGCACCA191120.2184218087181753No Hit
CAAAGAATTCTCCTTTTGGGCTT190200.21737038519357965No Hit
TGAGGTAGTAGTTTGTGCTGTT188250.2151418244620997No Hit
TATTGCACTTGTCCCGGCCTGTAAA184310.2106389889328531No Hit
ACTGGACTTGGAGTCAGAAGGCA181280.20717614841163048No Hit
CCCCCACTGCTAAATTTGACTGGCTT180440.20621615301960836No Hit
TTCTCACTACTGCACTTGACTAG179540.2051875865281561No Hit
ACTGGACTTGGAGTCAGAAGGCT173340.1981019062537071No Hit
AGCTACATCTGGCTACTGGGTCTC162650.1858848220385685No Hit
TTCAAGTAATCCAGGATAGGCTT161600.18468482779854084No Hit
AGCAGCATTGTACAGGGCTATGT155400.1775991475240919No Hit
TGTAAACATCCCCGACTGGAAGC148540.16975918515591126No Hit
TTCACAGTGGCTAAGTTCTG141620.16185065168830048No Hit
TTCAAGTAATCCAGGATAGGC135230.15454782959898936No Hit
TTATAAAGCAATGAGACTGATT134210.15338212090867678No Hit
CTTCTCACTACTGCACTTGACTAG133610.15269640991437528No Hit
CAACGGAATCCCAAAAGCAGCT132550.15148498715777595No Hit
TCAGTGCACTACAGAACTTTGT129370.1478507188879779No Hit
CCCATAAAGTAGAAAGCACTA129200.14765643410625914No Hit
CCCCCCACTGCTAAATTTGACTGGCT128920.1473364356422518No Hit
ACTGGACTTGGAGTCAGAAGG128010.1462964406342278No Hit
CAAAGTGCTGTTCGTGCAGGTAG125320.1432221696764427No Hit
ACCACAGGGTAGAACCACGGAC124880.14271931494728826No Hit
AGCAGCATTGTACAGGGCTATG120200.13737076919173646No Hit
TGAGGTAGTAGGTTGTGTGGTT116570.1332222176762123No Hit
AGCTACATTGTCTGCTGGGTTT113840.1301022326521404No Hit
TTCACAGTGGCTAAGTTCCGC112600.1286850965972506No Hit
CTCCCACTGCTTCACTTGACTAG109130.12471940134687354No Hit
CTACGCCTGTCTGAGCGTCGCT108650.12417083255143233No Hit
TATTGCACTTGTCCCGGCCTGA107270.12259369726453885No Hit
TTCACAGTGGCTAAGTTCTGC105620.1207079920302097No Hit
AACATTCAACGCTGTCGGTGAGA105340.1203879935662023No Hit
TACGCCTGTCTGAGCGTCGCT105290.12033085098334384No Hit
CAAAGTGCTGTTCGTGCAGGT105170.12019370878448354No Hit
AACATTCAACGCTGTCGGTGA104010.1188680008621673No Hit
ATGCACCTGGGCAAGGATTCTGA102810.11749657887356425No Hit
GAATACCGGGTGCTGTAGGCTTT101430.11591944358667078No Hit
TTCACCACCTTCTCCACCCAGC97420.11133660844142235No Hit
TAGCAGCACATCATGGTTTACA95940.10964518798881194No Hit
TATTGCACTTGTCCCGGCCTG95510.1091537617762292No Hit
ATCACATTGCCAGGGATTTCC93920.10733662764133019No Hit
ATCCCACCGCTGCCACCA93780.1071766284093265No Hit
GGAATACCGGGTGCTGTAGGCTTT93700.10708520027675296No Hit
TTAATGCTAATCGTGATAGGGGTTT93530.10689091549503418No Hit
TGAGGTAGTAGGTTGTATAGT92120.10527949465842565No Hit
TAGCAGCACGTAAATATTGGCGT92000.10514235245956532No Hit
TGAGGGGCAGAGAGCGAGACTTT90890.10387378712010753No Hit
TCAGTGCACTACAGAACTTTGTC87760.10029666143316798No Hit
TCCTGTACTGAGCTGCCCCGAG87520.10002237703544738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)