FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941118
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG123450812.418200850246421No Hit
TAGCAGCACGTAAATATTGGCG3953063.9764742758309475No Hit
AACATTCAACGCTGTCGGTGAGT3933513.9568084796901113No Hit
TTCAAGTAATCCAGGATAGGCT3689833.7116851444676544No Hit
AAGCTGCCAGTTGAAGAACTGT3228813.2479344878513663No Hit
CCCCCCACTGCTAAATTTGACTG3079013.097247211027975No Hit
AACATTCAACGCTGTCGGTGAG2962482.9800269949516744No Hit
TATTGCACTTGTCCCGGCCTGTAA2616442.6319373736434875No Hit
TATTGCACTTGTCCCGGCCTGTAT2378282.392366733801973No Hit
TAGCTTATCAGACTGATGTTGACT1881121.8922620172097344No Hit
TAGCTTATCAGACTGATGTTGAC1730361.7406090542331356No Hit
CCCCCACTGCTAAATTTGACTG1147621.1544174407747698No Hit
TAGCTTATCAGACTGATGTTGA1106271.1128225215715173No Hit
TGTAAACATCCTTGACTGGAAGCT1083551.0899679492789445No Hit
TGAGGTAGTAGGTTGTATAGTT948610.9542286893687411No Hit
TATTGCACTTGTCCCGGCCTGT937600.9431534762991446No Hit
TATTGCACTTGTCCCGGCCTGTA846930.8519464309748661No Hit
CAACGGAATCCCAAAAGCAGCTGT807700.8124840686932798No Hit
CATAAAGTAGAAAGCACTACT795570.8002822217782748No Hit
AGCAGCATTGTACAGGGCTATGA761610.7661210741085661No Hit
TGTAAACATCCCCGACTGGAAGCT602210.6057769357531014No Hit
TTCAAGTAATTCAGGATAGGTT571950.5753377034655459No Hit
CAACGGAATCCCAAAAGCAGCT557610.5609127665520116No Hit
CCCATAAAGTAGAAAGCACTAC543440.5466588365614411No Hit
TGAGGTAGTAGATTGTATAGTT526300.5294173150343855No Hit
CCCCCACTGCTAAATTTGACTGGCTT444620.44725351816566306No Hit
CACCCGGCTGTGTGCACATGTGC428140.4306759058689375No Hit
ACTGGACTTGGAGTCAGAAGGC393350.39567984204593487No Hit
TGTAAACATCCTACACTCTCAGCT392730.39505616973865515No Hit
CATTGCACTTGTCTCGGTCTGA385520.3878034643588377No Hit
CAAAGAATTCTCCTTTTGGGCT356750.35886305745490593No Hit
CCCATAAAGTAGAAAGCACT352720.35480918745758777No Hit
GCCTGTCTGAGCGTCGCT337590.33958957131380996No Hit
TAGCTTATCAGACTGATGTTGACA325460.32738772439880504No Hit
TTCTCACTACTGCACTTGACTAG299010.30078105903179103No Hit
TATTGCACTTGTCCCGGCCTGTAG288610.2903194590387117No Hit
AACATTCAACGCTGTCGGTGAGTT274490.27611582520195416No Hit
CTTCTCACTACTGCACTTGACTAG252360.25385474752437304No Hit
AACGGAATCCCAAAAGCAGCTG247740.24920738291206282No Hit
TGAGGTAGTAGTTTGTACAGTT241670.24310142983917904No Hit
TAGCAGCACGTAAATATTGGC239950.24137124214801595No Hit
CAACGGAATCCCAAAAGCAGCTGA234980.23637180445901557No Hit
CACTAGATTGTGAGCTCCTGGAA234230.23561736215182236No Hit
TATTGCACTTGTCCCGGCCTGTAAA232650.2340280036913353No Hit
CAACACCAGTCGATGGGCTGT228950.23030608830918214No Hit
TATTGCACTTGTCCCGGCCTGTT223260.22458238600527625No Hit
TACGCCTGTCTGAGCGTCGCT221430.22274154677572483No Hit
CTACGCCTGTCTGAGCGTCGCT212560.21381900908931972No Hit
CACTAGATTGTGAGCTCCTGGA210310.21155568216774007No Hit
TATTGCACTTGTCCCGGCCTGTAAT208830.2100669160148788No Hit
TATTGCACTTGTCCCGGCCTGTTT206250.20747163447813413No Hit
TCGTTTCCCGGCCAATGCACCA205400.20661659986331518No Hit
CTCCCACTGCTTCACTTGACTAG200610.2017982283280412No Hit
CAAAGAATTCTCCTTTTGGGCTT198570.19974614525247564No Hit
TTCACAGTGGCTAAGTTCCG198060.19923312448358427No Hit
CCCCCCACTGCTAAATTTGACTGGCT192410.1935496591027287No Hit
AGCTCGGTCTGAGGCCCCTCAGT191110.19224195910359376No Hit
TGATGTAGTAGGTTGTATAGTT183470.18455670680098554No Hit
ATGCACCTGGGCAAGGATTCTGA175700.17674068449846383No Hit
TAGCAGCACATCATGGTTTACA175480.1765193814216872No Hit
TTCACAGTGGCTAAGTTCCGC170190.17119804834828437No Hit
TGTCAGTTTGTCAAATACCCCA166190.1671743560432539No Hit
TGTCAGTTTGTCAAATACCCCAA164970.1659471298902196No Hit
ATCACATTGCCAGGGATTTCC164230.16520274681378894No Hit
TGTAAACATCCCCGACTGGAAGC163310.16427729758363194No Hit
TTCAAGTAATCCAGGATAGGCTT161320.16227551066187929No Hit
AGCAGCATTGTACAGGGCTATGT161310.1622654514311167No Hit
TGAGGTAGTAGTTTGTGCTGTT156190.15711512528067767No Hit
TTCAAGTAATCCAGGATAGGC155560.1564813937426354No Hit
TTCACAGTGGCTAAGTTCTGC154690.15560624066629125No Hit
CGCCTGTCTGAGCGTCGCT154560.15547547066637776No Hit
CCCATAAAGTAGAAAGCACTA146920.14779021836376954No Hit
GGAATACCGGGTGCTGTAGGCTTT140450.14128189606038274No Hit
ACTGGACTTGGAGTCAGAAGGCA135870.13667476837112286No Hit
TCAGTGCACTACAGAACTTTGT135080.13588008914087932No Hit
GTCAGGATGGCCGAGCGGTCTAAGG133590.13438126375725545No Hit
ATCACATTGCCAGGGATTTCCA132080.13286231991210645No Hit
AACATTCAACGCTGTCGGTGAGA129200.12996526145248452No Hit
ACTGGACTTGGAGTCAGAAGGCT125810.1265551822239712No Hit
TCCTGTCTGAGCGTCGCT124880.12561967376305158No Hit
TATTGCACTTGTCCCGGCCTGA123060.12378889376426273No Hit
CGTTCGCGCTTTCCCCTG118410.1191113514596648No Hit
CAAAGTGCTGTTCGTGCAGGTAG115810.11649595146139499No Hit
AATGCACCCGGGCAAGGATTCT115410.11609358223089195No Hit
TCGTACGACTCTTAGCGG115170.1158521606925901No Hit
TAGCAGCACGTAAATATTGGCGT113810.11448410530887974No Hit
TTCACAGTGGCTAAGTTCTG111540.11220065992577496No Hit
TGAGGTAGTAGGTTGTGTGGTT108640.10928348300462784No Hit
AACATTCAACGCTGTCGGTGA107590.10822726377455735No Hit
TGTAAACATCCTACACTCAGCT107490.10812667146693158No Hit
ATCCCGGACGAGCCCCCA106250.10687932685237214No Hit
CCCCCACTGCTAAATTTGACTGGCTA104390.10500830993053296No Hit
CTCCCACTGCTTCACTTGACTAGCCT102610.1032177668547944No Hit
AGCAGCATTGTACAGGGCTATG102070.10267456839361529No Hit
CAAAGTGCTGTTCGTGCAGGT101650.10225208070158709No Hit
ACCACAGGGTAGAACCACGGAC100780.10137692762524296No Hit
TGATGTAGTAGATTGTATAGTT100460.10105503224084052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)