FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675140
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG79315810.33411768384681No Hit
CCCCCCACTGCTAAATTTGACTG6752598.798002381715513No Hit
AACATTCAACGCTGTCGGTGAGT3213314.186646758235029No Hit
AACATTCAACGCTGTCGGTGAG2581143.362987515537176No Hit
TAGCAGCACGTAAATATTGGCG2528823.294819377887569No Hit
AAGCTGCCAGTTGAAGAACTGT2376423.0962562246421563No Hit
TTCAAGTAATCCAGGATAGGCT2108192.7467772574832514No Hit
TAGCTTATCAGACTGATGTTGAC1924242.5071073622109825No Hit
CCCCCACTGCTAAATTTGACTG1817412.367917718764739No Hit
TAGCTTATCAGACTGATGTTGACT1733712.258864333419325No Hit
TATTGCACTTGTCCCGGCCTGTAA1583942.0637278277660083No Hit
TATTGCACTTGTCCCGGCCTGTAT1371571.787029291973827No Hit
TAGCTTATCAGACTGATGTTGA1250621.6294425899723002No Hit
TATTGCACTTGTCCCGGCCTGT984181.2822958278285477No Hit
TGTAAACATCCTTGACTGGAAGCT774251.0087763871408209No Hit
GCCTGTCTGAGCGTCGCT763710.9950437386158427No Hit
TATTGCACTTGTCCCGGCCTGTA711410.9269016591228304No Hit
TGAGGTAGTAGGTTGTATAGTT637910.8311379336402984No Hit
AGCAGCATTGTACAGGGCTATGA583040.7596473810249715No Hit
CATAAAGTAGAAAGCACTACT553810.7215633851630068No Hit
TGTAAACATCCCCGACTGGAAGCT508800.6629195037484659No Hit
TGAGGTAGTAGATTGTATAGTT419090.5460356423465891No Hit
CAACGGAATCCCAAAAGCAGCTGT369790.4818022863426596No Hit
ACTGGACTTGGAGTCAGAAGGC347280.45247383109624056No Hit
TTCAAGTAATTCAGGATAGGTT347140.4522914240000834No Hit
CATTGCACTTGTCTCGGTCTGA346800.4518484353379873No Hit
TCGTACGACTCTTAGCGG345720.4504412948819174No Hit
CCCATAAAGTAGAAAGCACTAC325650.42429193473995264No Hit
CCCATAAAGTAGAAAGCACT315840.4115104089306514No Hit
TAGCTTATCAGACTGATGTTGACA271240.3534007197262851No Hit
TCCTGTCTGAGCGTCGCT268250.34950502531549915No Hit
AACATTCAACGCTGTCGGTGAGTT249690.3253230559963727No Hit
CAACACCAGTCGATGGGCTGT248930.32433284604580503No Hit
CACCCGGCTGTGTGCACATGTGC222600.29002728288995383No Hit
TATTGCACTTGTCCCGGCCTGTAG211100.2750438428484692No Hit
GGAATACCGGGTGCTGTAGGCTTT205870.26822963489916796No Hit
TGTAAACATCCTACACTCTCAGCT197680.25755881977397155No Hit
TATTGCACTTGTCCCGGCCTGTT194950.25400188139890606No Hit
CAAAGAATTCTCCTTTTGGGCT193740.25242536292497597No Hit
CCCCCACTGCTAAATTTGACTGGCTT180000.23452340934497612No Hit
TTCTCACTACTGCACTTGACTAG177900.23178730290261806No Hit
TGTCAGTTTGTCAAATACCCCAA170980.22277118072113342No Hit
CGCCTGTCTGAGCGTCGCT170120.22165067998759633No Hit
TGAGGTAGTAGTTTGTACAGTT167940.21881034091886273No Hit
AACGGAATCCCAAAAGCAGCTG157000.20455652926200693No Hit
ACCCCCCACTGCTAAATTTGACTG156520.20393113350375366No Hit
GGAATACCGGGTGCTGTAGGCTT155400.20247187673449607No Hit
CCCCCCACTGCTAAATTTGACTGG155390.20245884765619912No Hit
TGAGGTAGTAGTTTGTGCTGTT154410.2011819979830987No Hit
CTCCCACTGCTTCACTTGACTAG153300.199735770292138No Hit
TAGCAGCACGTAAATATTGGC151700.19765111776462707No Hit
TCGTTTCCCGGGCGGCGCACCA130480.17000341361851382No Hit
CTTCTCACTACTGCACTTGACTAG130140.16956042495641774No Hit
CAACGGAATCCCAAAAGCAGCT128720.1677102958382518No Hit
TATTGCACTTGTCCCGGCCTGTAAT125160.16307194396454006No Hit
CCCCCCACTGCTAAATTTGACTGGCT121420.15819906868148334No Hit
AGCAGCATTGTACAGGGCTATGT120170.15657043389436545No Hit
TATTGCACTTGTCCCGGCCTGTTT118380.15423822887921262No Hit
TTCAAGTAATCCAGGATAGGCTT116900.15230992529126505No Hit
AGCTCGGTCTGAGGCCCCTCAGT113590.14799730037497688No Hit
ATCCCGGACGAGCCCCCA112690.146824683328252No Hit
TATTGCACTTGTCCCGGCCTGTAAA112530.1466162180755009No Hit
TGTAAACATCCCCGACTGGAAGC112400.14644684005764064No Hit
CAAAGAATTCTCCTTTTGGGCTT112340.146368665587859No Hit
CAAAGTGCTGTTCGTGCAGGTAG112300.14631654927467122No Hit
TTCACAGTGGCTAAGTTCCG110680.1442058385905664No Hit
TGTCAGTTTGTCAAATACCCCA110320.14373679177187645No Hit
TAGCTTATCAGACTGATGTTGACC108400.14123520873886342No Hit
TTCAAGTAATCCAGGATAGGC101710.13251875535820845No Hit
CACTAGATTGTGAGCTCCTGGAA99440.12956115458480236No Hit
CCCATAAAGTAGAAAGCACTA98380.12818007228532638No Hit
AACCGGGCGGAAACACCA98340.1281279559721386No Hit
CACTAGATTGTGAGCTCCTGGA98120.12784131624960587No Hit
ACCACAGGGTAGAACCACGGAC98110.12782828717130892No Hit
AACATTCAACGCTGTCGGTGAGA96210.12535276229488973No Hit
ACTGGACTTGGAGTCAGAAGGCT95280.12414105801327403No Hit
ACTGGACTTGGAGTCAGAAGGCA93700.12208246364235702No Hit
TTCACAGTGGCTAAGTTCCGC93120.12132677710113432No Hit
ACTGGACTTGGAGTCAGAAGG92760.12085773028244437No Hit
TGATGTAGTAGGTTGTATAGTT92350.12032353807226968No Hit
AGCAGCATTGTACAGGGCTATG90860.11838220540602516No Hit
TTCACAGTGGCTAAGTTCTG90800.1183040309362435No Hit
TTATAAAGCAATGAGACTGATT89370.11644087273978065No Hit
TATTGCACTTGTCCCGGCCTGA86090.11216733505838329No Hit
CTACGCCTGTCTGAGCGTCGCT85780.11176343363117806No Hit
ATGCACCTGGGCAAGGATTCTGA84570.11018691515724795No Hit
ACCGGGTGCTGTAGGCTT83860.10926185059816498No Hit
AACATTCAACGCTGTCGGTGA82340.10728143069702963No Hit
CGCGACCTCAGATCAGACGTGGCG80640.10506648738654929No Hit
TAGCAGCACATCATGGTTTACA76810.10007635039882008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)