FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6320934
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5836459.233524665816791No Hit
CCCCCCACTGCTAAATTTGACTG3108064.917089784516023No Hit
AAGCTGCCAGTTGAAGAACTGT2351133.719592705761522No Hit
TTCAAGTAATCCAGGATAGGCT2288993.621284449418393No Hit
AACATTCAACGCTGTCGGTGAGT2028593.209320015048409No Hit
TAGCAGCACGTAAATATTGGCG1995073.156289877413686No Hit
AACATTCAACGCTGTCGGTGAG1641842.5974642355069677No Hit
CCCCCACTGCTAAATTTGACTG1145161.811694284420625No Hit
TATTGCACTTGTCCCGGCCTGT918541.4531713193018627No Hit
TAGCTTATCAGACTGATGTTGA886721.402830657621168No Hit
TAGCTTATCAGACTGATGTTGAC845531.3376662372997408No Hit
TGAGGTAGTAGGTTGTATAGTT777381.2298498924367824No Hit
CATAAAGTAGAAAGCACTACT719371.1380754806172633No Hit
GCCTGTCTGAGCGTCGCT717341.1348639299192176No Hit
TATTGCACTTGTCCCGGCCTGTAA664281.0509206392599575No Hit
TATTGCACTTGTCCCGGCCTGTAT579420.9166683278135794No Hit
TAGCTTATCAGACTGATGTTGACT570060.9018603896196353No Hit
CAACGGAATCCCAAAAGCAGCT530450.8391955998907756No Hit
TGAGGTAGTAGATTGTATAGTT473190.7486077215803867No Hit
ATCCCGGACGAGCCCCCA446700.7066993580379102No Hit
CCCATAAAGTAGAAAGCACT437400.6919863425246965No Hit
TATTGCACTTGTCCCGGCCTGTA423970.6707394824878729No Hit
TGTAAACATCCTTGACTGGAAGCT366490.5798035543481391No Hit
CGCCTGTCTGAGCGTCGCT366340.579566247646313No Hit
ACCGGGTGCTGTAGGCTT357620.5657708180468266No Hit
AGCAGCATTGTACAGGGCTATGA345110.545979439114536No Hit
TCGTACGACTCTTAGCGG343760.5438436787981017No Hit
CATTGCACTTGTCTCGGTCTGA325730.5153194132386132No Hit
TACGCCTGTCTGAGCGTCGCT323210.5113326606479358No Hit
TTCAAGTAATTCAGGATAGGTT322400.5100512044580754No Hit
CAACACCAGTCGATGGGCTGT288000.455628867505973No Hit
CAACGGAATCCCAAAAGCAGCTGT279520.44221312862940826No Hit
CGTTCGCGCTTTCCCCTG240820.3809879995582931No Hit
TGAGGTAGTAGTTTGTACAGTT236660.37440669369431795No Hit
TCCTGTCTGAGCGTCGCT234830.37151155193204044No Hit
CCCATAAAGTAGAAAGCACTAC224490.3551532099528329No Hit
CTACGCCTGTCTGAGCGTCGCT209060.3307422605583289No Hit
TGTAAACATCCCCGACTGGAAGCT208760.3302676471546768No Hit
ACCGGGTGCTGTAGGCTTT207090.32762563254101373No Hit
ACTGGACTTGGAGTCAGAAGGC192190.30405316682629496No Hit
ACCGGGTGCTGTAGGCTTA185690.2937698764138338No Hit
CACCCGGCTGTGTGCACATGTGC183850.29085891420476784No Hit
TTCACAGTGGCTAAGTTCCG172990.27367790899256345No Hit
AACGGAATCCCAAAAGCAGCTG161640.25572170188772736No Hit
TGAGGTAGTAGTTTGTGCTGTT157730.24953590719346222No Hit
TTCTCACTACTGCACTTGACTAG155590.24615033158074426No Hit
TGTAAACATCCTACACTCTCAGCT151870.24026512537545877No Hit
TCGTTTCCCGGCCAATGCACCA146270.23140567517395372No Hit
TGAGAACTGAATTCCATGGGTT137150.21697742770293124No Hit
TTCACAGTGGCTAAGTTCCGC137080.21686668457541244No Hit
ATCCCACCGCTGCCACCA128720.20364079106030847No Hit
TATTGCACTTGTCCCGGCCTGA127470.20166323521175825No Hit
TATTGCACTTGTCCCGGCCTGTT123180.19487626353953388No Hit
TTCAAGTAATCCAGGATAGGC121030.19147486748002748No Hit
CTCCCACTGCTTCACTTGACTAG119400.18889613465351798No Hit
AACATTCAACGCTGTCGGTGAGTT117660.18614337691233604No Hit
CAACGGAATCCCAAAAGCAGCTGA112950.17869194647499878No Hit
TTCACAGTGGCTAAGTTCTGC111970.17714154268973542No Hit
AGCTCGGTCTGAGGCCCCTCAGT108030.17090828665510507No Hit
TGAGGTAGTAGGTTGTGTGGTT107410.16992741895422417No Hit
TCCCCGGCACCTCCACCA102750.1625550907508289No Hit
AACCGGGCGGAAACACCA100050.1582835701179604No Hit
AATGCACCCGGGCAAGGATTCT98840.1563692960565638No Hit
TAGCAGCACGTAAATATTGGC97630.15445502199516717No Hit
TTAATGCTAATCGTGATAGGGGTT95710.151417496211794No Hit
TGAGGTAGTAGGTTGTATAGT89510.14160881920298488No Hit
CCCATAAAGTAGAAAGCACTA88440.13991603139662587No Hit
AATGACACGATCACTCCCGTTG87450.1383498071645741No Hit
TAGCTTATCAGACTGATGTTGACA87100.13779609152698005No Hit
CTTCTCACTACTGCACTTGACTAG85140.13469528395645328No Hit
TTCACAGTGGCTAAGTTCTG85130.13467946350966487No Hit
ACCACAGGGTAGAACCACGGAC85020.13450543859499245No Hit
AGCAGCATTGTACAGGGCTATGT84380.13349293000053472No Hit
AGCAGCATTGTACAGGGCTATG81840.12947453651628066No Hit
CACCCGTAGAACCGACCTTGCG81640.12915812758051262No Hit
TGTAAACATCCTACACTCAGCT80410.12721221262553922No Hit
CGCGACCTCAGATCAGACGTGG79100.12513973409625856No Hit
GGAATACCGGGTGCTGTAGGCTTT77320.12232369456792304No Hit
GGAATACCGGGTGCTGTAGGCTT77140.1220389265257318No Hit
TTCAAGTAATCCAGGATAGGCTT76560.1211213406120045No Hit
ACGCCTGTCTGAGCGTCGCT76060.12033031827258439No Hit
TATTGCACTTGTCCCGGCCTGTAG74600.11802053304147772No Hit
AGAGGTAGTAGGTTGCATAGTT72500.11469823921591335No Hit
CCCCCACTGCTAAATTTGACTGGCTT72460.11463495742875973No Hit
CCCCCCACTGCTAAATTTGACTGG72280.11435018938656852No Hit
TCGAGGAGCTCACAGTCTAGT70520.11156579075180977No Hit
TTATAAAGCAATGAGACTGATT69010.1091769032867611No Hit
TAGCAGCACATCATGGTTTACA68950.10908198060603069No Hit
CAAAGTGCTGTTCGTGCAGGT68610.10854408541522502No Hit
AACATTCAACGCTGTCGGTGA67890.1074050132464601No Hit
TGTAAACATCCCCGACTGGAAGC67450.1067089135877704No Hit
AACATTCAACGCTGTCGGTGAGA67400.1066298113538284No Hit
TGATGTAGTAGGTTGTATAGTT66210.1047471781860086No Hit
CAAAGAATTCTCCTTTTGGGCT64410.10189949776409626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)